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Rabbit anti pchk1 s317

Manufactured by Cell Signaling Technology
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The Rabbit anti-pChk1 (S317) is a primary antibody that specifically recognizes the phosphorylated form of the Chk1 protein at serine 317. It is a useful tool for detecting and quantifying the active, phosphorylated state of Chk1 in biological samples.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) Bis tris gradient gel, by Thermo Fisher Scientific (2 mentions) Rabbit anti pchk2 t68, by Cell Signaling Technology (2 mentions) Mouse anti tubulin e7, by Developmental Studies Hybridoma Bank (2 mentions) Rabbit anti vp1, by Abcam (1 mentions) Rabbit anti gapdh, by Abcam (1 mentions)

Close spelling variants of this product

Anti-pChk1(S317)

3 protocols using rabbit anti pchk1 s317

1

Western Blot Antibody Protocol

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Primary antibodies used for western blot were: rabbit anti-Rad50 (GeneTex, GTX119731) 1:1000; rabbit anti- pCHK1 S317 (Cell Signaling, 12302P) 1:200; rabbit anti-pCHK1 S296 (Cell Signaling, 2349) 1:1000, rabbit anti-GAPDH (Abcam, 37168) 1:1000; mouse anti-Tag, PN116 (a gift from Brian Schaffhausen), 1:250; rabbit anti-VP1 (I58), 1:5000 (Montross, et al. 1991 (link)).
Heiser K., Nicholas C, & Garcea R.L. (2016). Activation of DNA Damage Repair Pathways by Murine Polyomavirus. Virology, 497, 346-356.
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2

Western Blot Analysis of Cell Signaling

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Cells were harvested with RIPA buffer (Cold Springs Harbor protocols) containing protease and phosphatase inhibitors, and proteins were separated on a 4%–12% Bis-Tris gradient gels (Thermo-Fisher). and transferred to Pure Nitrocellulose Blotting Membrane (Life Sciences) or Amersham™ Hybond™ PVDF membrane (0.45 μM GE Healthcare Life Science). Membranes were blocked with 4% BSA, incubated with primary antibody overnight (1:1000–1:2000 dilution), washed, and incubated with secondary fluorescent conjugate anti-bodies or HRP, followed by a last wash. Membranes were scanned using LI-COR Odyssey CLX, Bio-rad Chemidoc™ or exposed to film.
Primary antibodies used are: Mouse monoclonal anti-p53 (Santa Cruz Biotechnology Cat# sc-126, RRID:AB_628082), mouse monoclonal anti-MDM2 (Ab-1, Millipore Cat# OP46T-10UG, RRID:AB_564805), rabbit anti-pChk2 (T68) (Cell Signaling Technology), rabbit anti-pChk1 (S317) (Cell Signaling Technology), mouse anti-tubulin (E7) (Developmental Studies Hybridoma Bank), mouse monoclonal anti-β-Actin (AC-74) (Sigma-Aldrich Cat# A2228, RRID:AB_476697). Info on Secondary antibodies?
Tsabar M., Mock C.S., Venkatachalam V., Reyes J., Karhohs K.W., Oliver T.G., Regev A., Jambhekar A, & Lahav G. (2020). A Switch in p53 Dynamics Marks Cells That Escape from DSB-Induced Cell Cycle Arrest. Cell reports, 32(5), 107995.
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3

Western Blot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested with RIPA buffer (Cold Springs Harbor protocols) containing protease and phosphatase inhibitors, and proteins were separated on a 4%–12% Bis-Tris gradient gels (Thermo-Fisher). and transferred to Pure Nitrocellulose Blotting Membrane (Life Sciences) or Amersham™ Hybond™ PVDF membrane (0.45 μM GE Healthcare Life Science). Membranes were blocked with 4% BSA, incubated with primary antibody overnight (1:1000–1:2000 dilution), washed, and incubated with secondary fluorescent conjugate anti-bodies or HRP, followed by a last wash. Membranes were scanned using LI-COR Odyssey CLX, Bio-rad Chemidoc™ or exposed to film.
Primary antibodies used are: Mouse monoclonal anti-p53 (Santa Cruz Biotechnology Cat# sc-126, RRID:AB_628082), mouse monoclonal anti-MDM2 (Ab-1, Millipore Cat# OP46T-10UG, RRID:AB_564805), rabbit anti-pChk2 (T68) (Cell Signaling Technology), rabbit anti-pChk1 (S317) (Cell Signaling Technology), mouse anti-tubulin (E7) (Developmental Studies Hybridoma Bank), mouse monoclonal anti-β-Actin (AC-74) (Sigma-Aldrich Cat# A2228, RRID:AB_476697). Info on Secondary antibodies?
Tsabar M., Mock C.S., Venkatachalam V., Reyes J., Karhohs K.W., Oliver T.G., Regev A., Jambhekar A, & Lahav G. (2020). A Switch in p53 Dynamics Marks Cells That Escape from DSB-Induced Cell Cycle Arrest. Cell reports, 32(5), 107995.
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