Monoclonal mouse anti human gfap antibody

The vertebra segments were harvested from six experimental models of each time point, post-fixed, and sectioned. Sections were allowed to incubate with monoclonal MIF antibody (1:200 dilution), rabbit anti-IBA-1 antibody (1:400 dilution, Wako), polyclonal rabbit anti-CCL5 antibody (1:200 dilution, novusbio), or monoclonal mouse anti-human GFAP antibody (1:400 dilution, Sigma) at 4 °C for 36 h. The sections were further reacted with the FITC-labeled secondary antibody goat anti-mouse IgG (1:400 dilution, Gibco) or the TRITC-labeled secondary antibody donkey anti-rabbit IgG (1:400 dilution, Gibco) at 4 °C overnight, followed by observation under a confocal laser scanning microscope (Leica, Heidelberg, Germany).

The vertebra segments were harvested from six experimental models of each time point, post-fixed, and sectioned. Sections were allowed to incubate with polyclonal COX2 antibody (1:100 dilution, Cayman), goat anti-IBA-1 antibody (1:200 dilution, Abcam), monoclonal mouse anti-S-100 (β-Subunit) antibody (1:1000 dilution, Sigma), or monoclonal mouse anti-human GFAP antibody (1:400 dilution, Sigma) at 4 °C for 36 h. The sections were further reacted with the FITC-labeled secondary antibody goat anti-mouse IgG (1:400 dilution, Gibco), the TRITC-labeled secondary antibody donkey anti-rabbit IgG (1:400 dilution, Gibco), or the 488-labeled secondary antibody donkey anti-goat IgG (1:400 dilution, Abcam) at 4 °C overnight, followed by observation under a confocal laser scanning microscope (Leica, Heidelberg, Germany).