Fitc conjugated cd15

The leukocytes isolated from breast cancer patients and healthy controls were resuspended in 100 μl PBS and stained with master mix of Abs for cellular surface stains including FITC‐conjugated CD15 and phycoerythrin‐conjugated CD11b (both from BD Biosciences) for 30 min at 4°C. The stained cells were analyzed by FACSCalibur flow cytometer (Becton Dickinson).

TNCs (2 × 10⁵) were labeled with a combination of PerCP‐conjugated CD45 (BD Biosciences, clone 2D1) and APC‐conjugated CD34 (BD Biosciences, clone 8G12) plus: FITC‐conjugated CD14 (BD Biosciences, clone MSE2) for monocyte detection; PhycoErythrin (PE)‐conjugated CD56 (BD Biosciences, clone MY31), FITC‐conjugated CD2 and CD3 (BD Biosciences, clone MOPC21 and UCHT1), FITC‐conjugated CD19 and CD20 (BD Biosciences, clone HIB19 and 2H7) for lymphocyte detection; and FITC‐conjugated CD15 (BD Biosciences, clone MMA) for granulocyte detection. The percentage of cell impurities was calculated as the percentage of CD45⁺ events after the exclusion of CD34⁺ events using a FACS Canto II analyzer (BD Biosciences) and FACS DIVA software (Supporting Information Fig. S2).