Accu check nano

IPGTT was performed on mice fasted for 6 hrs. Blood samples were obtained at 0 and 10, 20, 30, 45, 60, 90, and 120 min post-intraportal glucose injection (2 g/kg body weight). Blood glucose levels were measured using an Accu-Check Nano glucometer and Smart View test strips (Roche). IPGTT was calculated by estimating the total area under the curve using GraphPad trapezoid rule.

After 20 weeks of diet, mice were fasted on wire-bottom cages for 4h or 6h prior to insulin or glucose tolerance test (ITT or GTT), respectively. For ITT, mice were injected with human insulin (Humulin R, Lilly USA, Indianapolis, IN); all mice were given the same volume of insulin based on a dose of 1 U/kg calculated for the average body weight of the LFD group. For GTT, all mice were injected with the same volume of 20% glucose solution, based on a 1g/kg dose calculated for the average body weight of the LFD group. Blood glucose levels were assessed by tail vein micropuncture using a hand-held glucometer (Accu-Check Nano, Roche, Basel, Switzerland).

Blood glucose and ketone concentrations were assessed via tail vein micropuncture using a hand-held glucometer (Accu-Check Nano, Roche, Basel, Switzerland) or ketone meter (novaMax Plus, Nova Biomedical, Waltham, MA). These analyses were performed in separate sets of mice subjected to CS-induced sepsis as described above.