Power lab 8 sp data

Cystometric and manometry data were recorded using LabChart software (versions 7 and 8) through a PowerLab/8SP data acquisition system (ADInstruments, Australia). Data were analyzed using Excel (Microsoft, Redmond, WA) and Prism 6 (GraphPad Software Inc., San Diego, CA). Data were subjected to ANOVA with Dunnett’s multiple comparison test or Sidak’s multiple comparison test to compare the effect of test compounds with vehicle, or Student’s t-test, as appropriate; P < 0.05 was considered statistically significant. Bioanalytical data were collected using Analyst (MDS Sciex, Framingham, MA, USA). Regression analysis was generated using Watson Laboratory Information Management System (ThermoFisher Scientific, Logan, UT, USA) and Excel (Microsoft, Redmond, WA, USA). Noncompartmental PK analysis was performed using Phoenix WinNonlin 6.3 (Certara, St. Louis, MO, USA) or PK Solver (Zhang et al, 2010 ). AUC was calculated between the first and last sample time points. All values are expressed as the mean + standard deviation (S.D.), except the cardiovascular data in Fig. 6 which is presented as mean + standard error (S.E.).