Dmem glutamax with sodium pyruvate

Manufactured by Thermo Fisher Scientific

DMEM GlutaMAX with sodium pyruvate is a cell culture medium designed to support the growth and maintenance of a variety of cell types. It contains the essential nutrients, amino acids, and vitamins required for cell proliferation, while also providing a source of energy through the inclusion of sodium pyruvate.

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Lab products found in correlation

2 mercaptoethanol, by Merck Group (2 mentions) Pd0325901, by STEMCELL (1 mentions) Lif supplement, by Merck Group (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Chir99021, by Cambridge Bioscience (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)

2 protocols using dmem glutamax with sodium pyruvate

Mouse E14Tg2A ESCs Maintenance and Modification

Cited 2 times

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Mouse E14Tg2A (E14) ESCs (male) were used for all experiments (Hooper et al. 1987 (link)) and to derive 2C-GFP reporter cells. 2C-GFP reporter ESCs were described previously (Percharde et al. 2018 (link)) and were used when prior purification of larger numbers of 2C-like cells was not needed or for validation. All ESCs were cultured at 37°C with 5% CO₂ on 0.1% gelatin-coated plates in ES-FBS culture medium (high-glucose DMEM GlutaMAX with sodium pyruvate [Thermo Fisher Scientific], 15% FBS [Gibco], 0.1 mM nonessential amino acids [Gibco], 0.1 mM 2-mercaptoethanol [Millipore], 1000 U/mL LIF supplement [ESGRO, Millipore]). Experiments comparing wild-type versus Dux^−/− E14 ESCs were performed in FBS/LIF media as above, supplemented with 2i (1 µM PD0325901 [Stem Cell Technologies], 3 µM CHIR99021 [Cambridge Bioscience]) as in Grow et al. (2021) (link). ESCs were routinely tested for mycoplasma and found to be negative. Inhibitors (Supplemental Table S2) were added to ESCs at the indicated concentrations and durations unless otherwise explicitly mentioned in the figure legends.

Xie S.Q., Leeke B.J., Whilding C., Wagner R.T., Garcia-Llagostera F., Low Y., Chammas P., Cheung N.T., Dormann D., McManus M.T, & Percharde M. (2022). Nucleolar-based Dux repression is essential for embryonic two-cell stage exit. Genes & Development, 36(5-6), 331-347.

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Mouse Embryonic Stem Cell Culture

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Mouse E14Tg2A (E14) ES cells (male) were used for all experiments (Hooper et al., 1987 (link)), except for p53⁻^/⁻ ES cells (Sabapathy et al., 1997 (link)), which are V6.5-derived (male, a gift from Scott Oakes). ES cells were cultured on 0.1% gelatin-coated plates in ES-FBS culture medium (high glucose DMEM GlutaMAX with sodium pyruvate (Thermo Fisher Scientific), 15% FBS (Atlanta Biologicals), 0.1mM non-essential amino acids, 50 U/mL penicillin-streptomycin (UCSF Cell Culture Facility), 0.1mM 2-Mercaptoethanol (Millipore) and 1,000U/ml LIF supplement (ESGRO, Millipore). Where indicated, ES cells were grown in N2B27/2i/LIF conditions (DMEM/F-12, Neurobasal medium, 1x N2/B27 supplements, 1μM PD0325901, 3μM CHIR99021, LIF as above) according to (Ying et al., 2008 (link)) for at least 4 passages before being used for experiments. Deletion of Kap1 in CreERT2;Kap1^fl/fl ES cells (undetermined sex, Rowe et al., 2010 (link)) was performed in ES/FBS conditions with 1μM 4-OHT overnight and analyzed 4 days later. Routine testing of E14 ES cells revealed absence of mycoplasma contamination. ES cells were not genotyped.

Percharde M., Lin C.J., Yin Y., Guan J., Peixoto G.A., Bulut-Karslioglu A., Biechele S., Huang B., Shen X, & Ramalho-Santos M. (2018). A LINE1-Nucleolin partnership regulates early development and ES cell identity. Cell, 174(2), 391-405.e19.

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