LoVo cells were plated in six-well plates at a density of 2×105 cells/well overnight, and then cells were treated with different concentrations of PB2 (range 6–24 μM) for 48 hrs. Subsequently, the cells were harvested by trypsinization and washed once with cold PBS. The cells were subsequently treated with PI and anti-annexin-V antibody (Becton Dickinson, USA) at 4°C for 1 hr. Subsequently, cells were washed once with PBS and analyzed by flow cytometer.
Anti annexin 5 antibody
Manufactured by BD
Sourced in United States
The Anti-Annexin V antibody is a laboratory reagent designed for the detection and quantification of Annexin V, a protein involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and ELISA to study the expression and localization of Annexin V in biological samples.
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Lab products found in correlation
5 protocols using anti annexin 5 antibody
1
PB2 Cytotoxicity in LoVo Cells
2
Apoptosis Detection by Flow Cytometry
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Cells were harvested by trypsinization, washed once with cold PBS. Then, the cells were stained with PI (propidium iodide) and anti-Annexin-V antibody (Becton Dickinson, USA) at 4 °C for 1 h. Subsequently, cells were washed once with PBS and analyzed by FACS (BD, USA).
3
Apoptosis Analysis by Flow Cytometry
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At 48 h post-transfection, the 5-8F cells were washed, resuspended, and harvested. Subsequently, staining of the cells was performed with propidium iodide and anti-Annexin V antibody (BD Biosciences, Franklin Lakes, NJ, USA). Cell apoptosis was identified using flow cytometry.
4
Apoptosis Analysis of Tumor Cells
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Tumor cells in the logarithmic growth phase were extracted from each treatment group to prepare a single-cell suspension and inoculated in the 50 mL culture flask (2×105). Then the culture was incubated at 37°C. Cells were collected after 48 hours and washed twice with PBS, the sediment of which was conserved with 300 μL DNA dye for 30 minutes at room temperature without light. Cells were stained with propidium iodide and anti-annexin-V antibody (BD Biosciences, San Jose, CA, USA) following the manufacturer’s protocol, and stained cells were detected by flow cytometry.
5
Annexin V and PI Apoptosis Assay
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Cell lines were treated with the indicated concentrations of amiodarone and ABT-263. After 24 hours at 37°C, samples were clarified by centrifugation. Cells were suspended in annexin V binding buffer (1 mM HEPES, 15 mM NaCl and 0.25 mM CaCl 2 ) and stained with anti-annexin V antibody (BD Biosciences) and propidium iodide (PI) (Sigma-Aldrich). After 20 minutes at 4°C, samples were analyzed using a FACScan Fluorescence Activated Cell Analyzer (Becton Dickinson) and FlowJo software.
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