Alexa fluor 555 goat anti mouse igg antibody

Whole-mount immunostaining of zebrafish embryos was performed as described previously^{41 (link)}. Anti-Hu C/D antibody (BD, 1:500) was used as a primary antibody, and Alexa Fluor 555 goat anti-mouse IgG antibody (Molecular Probes) was used as a secondary antibody. Nuclei were counterstained with DAPI (Molecular Probes). These specimens were observed by a confocal microscope (Olympus FV1000).

After culturing the kidney for the desired amount of time, they were fixed in cold methanol at −20 °C overnight, washed 3 times in PBS before the primary antibody (mouse monoclonal anti-Trim28/Kap1 [20C1] antibody ChIP Grade (Abcam), rabbit monoclonal anti-Cbx5/HP1 alpha antibody (Abcam), rabbit polyclonal to Cbx1/HP1 beta (Abcam), rabbit polyclonal to Cbx3/HP1 gamma (Abcam), Rabbit monoclonal anti-laminin antibody (Sigma)), in PBS was added and incubated at 4 °C overnight. The kidneys were washed for 4 h in PBS and the secondary antibody (Molecular Probes Alexa Fluor 555 goat anti-mouse IgG antibody, Alexa Fluor 555 goat anti-rabbit IgG or Alexa Fluor 555 donkey anti-goat IgG (1:500)) was added overnight at 4 °C. The UB was stained using lectin Dolichos biflorus agglutinin (DBA) for at least 3 h. After incubation, the kidneys were washed for 3 h in PBS and embedded for microscopic analysis.