Vancomycin bodipy fl conjugate

These experiments were performed as previously described^{62 (link)}. H. pylori liquid cultures were harvested by centrifugation at 6000 g for 30 min. The medium was filtered by 0.45 μm filters and then subjected to ultracentrifugation at 36,000 r.p.m. in a Type 45 Ti Rotor for 1 h. Pellets were washed with PBS and combined. After another step of ultracentrifugation, the pellets were re-suspended in F12 medium, and protein was quantitated by a DC™ protein assay kit. Samples were stored at −20 °C until used. For imaging of OMVs, OMVs were stained with Vancomycin BODIPY™ FL Conjugate (Thermo Fisher, v34850).

Teicoplanin, vancomycin, oritavancin, and chloramphenicol (Sigma-Aldrich, Germany); dalbavancin (MedChemExpress, Sweden); MA79 (Csávás et al., 2015 (link)), ERJ390 (Pintér et al., 2009 (link)), and SZZS-12 (Szucs et al., 2017 (link)); carbenicillin, gentamicin and erythromycin (Duchefa Biochemie, Netherland); vancomycin BODIPY-FL conjugate (Thermo Fisher Scientific, Germany) and fluorescently labeled Teicoplanin (Vimberg et al., 2019 (link)).