Anti rat igg irdye 800cw

Manufactured by LI COR

The Anti-rat IgG IRDye 800CW is a fluorescently-labeled secondary antibody that binds to rat immunoglobulin G (IgG). It is designed for use in immunoassays and Western blotting applications that require detection of rat proteins.

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Lab products found in correlation

Irdye 800cw anti mouse igg, by LI COR (2 mentions) Anti β actin, by LI COR (1 mentions) Anti mouse igg irdye 680cw, by LI COR (1 mentions) Anti ha, by Roche (1 mentions) Image studio, by LI COR (1 mentions) Odyssey clx, by LI COR (1 mentions) Anti gfp, by Cell Signaling Technology (1 mentions) Ripa buffer, by Merck Group (1 mentions) Complete mini edta free protease inhibitor cocktail, by Roche (1 mentions)

Close spelling variants of this product

IRDye 800CW (808 citations) IRDye 800CW goat anti-rat IgG (10 citations) IgG IRDye 800CW (3 citations)

2 protocols using anti rat igg irdye 800cw

Western Blot Antibody Validation

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Anti-HA (Roche, 3F10, 11867423001, 1:1000), anti-GFP (Cell Signaling Technology, 2956, 1:1000), and anti-β-Actin (LI-COR Biosciences, 926-42212, 1:1000) were used as primary antibodies. As secondary antibodies, anti-rat IgG IRDye 800CW (LI-COR Biosciences, 926-32219, 1:10,000), anti-mouse IgG IRDye 680CW (LI-COR Biosciences, 926-68070, 1:10,000), anti-mouse IgG IRDye 800CW (LI-COR Biosciences, 925-32210, 1:10,000), and anti-rabbit IgG IRDye680CW (LI-COR Biosciences, 925-68071, 1:10,000) were used. Images were acquired with an Odyssey CLx (LI-COR Biosciences) and Image Studio (LI-COR Biosciences, version 5.2). Antibodies were validated by the manufacturers.

Apostolopoulos A., Kawamoto N., Chow S.Y., Tsuiji H., Ikeuchi Y., Shichino Y, & Iwasaki S. (2024). dCas13-mediated translational repression for accurate gene silencing in mammalian cells. Nature Communications, 15, 2205.

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Western Blot Analysis of FA2H, MLKL, and GAPDH

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Cell lysates were prepared in RIPA buffer (Sigma-Aldrich) supplemented with cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail (Roche) according to the manufacturers’ instructions. Blots were stained with monoclonal mouse anti-human FA2H (clone OTI1C5, catalogue number TA506818, Origene), monoclonal rat anti-MLKL (clone 3H1, catalogue number MAC604, Millipore), monoclonal mouse anti-HA (clone 6E2, catalogue number 2367S, Cell Signaling Technology), and monoclonal rabbit anti-human GAPDH (clone 14C10, catalogue number 2118S, Cell Signaling Technology). The secondary antibodies used were anti-mouse IgG-IRDye® 800CW (catalogue number 926-32210) or IgG-IRDye® 680LT (catalogue number 926-68020), anti-rat IgG-IRDye® 800CW (catalogue number 926-32219), and anti-rabbit IgG-IRDye® 680LT (catalogue number 926-68021; all from LI-COR Biosciences).

Faergeman S.L., Evans H., Attfield K.E., Desel C., Kuttikkatte S.B., Sommerlund M., Jensen L.T., Frokiaer J., Friese M.A., Matthews P.M., Luchtenborg C., Brügger B., Oturai A.B., Dendrou C.A, & Fugger L. (2020). A novel neurodegenerative spectrum disorder in patients with MLKL deficiency. Cell Death & Disease, 11(5), 303.

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