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Alexa fluor647 conjugated goat anti human igg

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Alexa Fluor647-conjugated goat anti-human IgG is a secondary antibody that binds to human IgG. It is conjugated with the Alexa Fluor 647 fluorescent dye, which can be detected using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Cd14 magnetic microbeads, by Miltenyi Biotec (2 mentions) Cd19 pe cy7 clone sj25c1, by Beckman Coulter (2 mentions) Cd4 pe texasred clone s3.5, by Thermo Fisher Scientific (2 mentions) Ccr7 bv421 clone g043h7, by BioLegend (2 mentions) Facsaria 3, by BD (2 mentions)

2 protocols using alexa fluor647 conjugated goat anti human igg

1

Purification of Memory Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocytes were isolated from PBMCs by positive selection using CD14
magnetic microbeads (Miltenyi Biotech). CD14-depleted fractions were stained on
ice for 15–20 min with the following fluorochrome-labeled mouse
monoclonal antibodies: CD8-PE–Cy5 (clone B9.11; cat. no. A07758),
CD14-PE–Cy5 (clone RMO52; cat. no. A07765), CD16-PE–Cy5 (clone
3G8; cat. no. A07767), CD45RA-FITC (clone ALB11; cat. no. A07786) from Beckman
Coulter, CD19-PE–Cy7 (clone SJ25C1; cat. no. 341113), CD25–PE
(clone M-A251; cat. no. 555432) from BD Biosciences, CD4–PE–Texas
Red (clone S3.5; cat. no. MHCD0417) from ThermoFisher Scientific,
CCR7–BV421 (clone G043H7; cat. no. 353208) from BioLegend, Alexa Fluor
647-conjugated goat anti-human IgG (cat. no. 109-606-170) from Jackson
ImmunoResearch. IgG+ memory B cells were sorted from CD19+B cells to over 98% purity on a FACSAria III (BD). Memory CD4+ T
cells were sorted to over 98% purity after exclusion of naïve
CD4+ T cells (CD45RA+CCR7+) and
CD8+, CD14+, CD16+, CD19+,
CD25bright cells.
Cassotta A., Mikol V., Bertrand T., Pouzieux S., Le Parc J., Ferrari P., Dumas J., Auer M., Deisenhammer F., Gastaldi M., Franciotta D., Silacci-Fregni C., Fernandez Rodriguez B., Giacchetto-Sasselli I., Foglierini M., Jarrossay D., Geiger R., Sallusto F., Lanzavecchia A, & Piccoli L. (2019). A single T cell epitope drives the neutralizing anti-drug antibody response to natalizumab in multiple sclerosis patients. Nature medicine, 25(9), 1402-1407.
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2

Purification of Memory Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocytes were isolated from PBMCs by positive selection using CD14
magnetic microbeads (Miltenyi Biotech). CD14-depleted fractions were stained on
ice for 15–20 min with the following fluorochrome-labeled mouse
monoclonal antibodies: CD8-PE–Cy5 (clone B9.11; cat. no. A07758),
CD14-PE–Cy5 (clone RMO52; cat. no. A07765), CD16-PE–Cy5 (clone
3G8; cat. no. A07767), CD45RA-FITC (clone ALB11; cat. no. A07786) from Beckman
Coulter, CD19-PE–Cy7 (clone SJ25C1; cat. no. 341113), CD25–PE
(clone M-A251; cat. no. 555432) from BD Biosciences, CD4–PE–Texas
Red (clone S3.5; cat. no. MHCD0417) from ThermoFisher Scientific,
CCR7–BV421 (clone G043H7; cat. no. 353208) from BioLegend, Alexa Fluor
647-conjugated goat anti-human IgG (cat. no. 109-606-170) from Jackson
ImmunoResearch. IgG+ memory B cells were sorted from CD19+B cells to over 98% purity on a FACSAria III (BD). Memory CD4+ T
cells were sorted to over 98% purity after exclusion of naïve
CD4+ T cells (CD45RA+CCR7+) and
CD8+, CD14+, CD16+, CD19+,
CD25bright cells.
Cassotta A., Mikol V., Bertrand T., Pouzieux S., Le Parc J., Ferrari P., Dumas J., Auer M., Deisenhammer F., Gastaldi M., Franciotta D., Silacci-Fregni C., Fernandez Rodriguez B., Giacchetto-Sasselli I., Foglierini M., Jarrossay D., Geiger R., Sallusto F., Lanzavecchia A, & Piccoli L. (2019). A single T cell epitope drives the neutralizing anti-drug antibody response to natalizumab in multiple sclerosis patients. Nature medicine, 25(9), 1402-1407.
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