L phenylalanine

Chicken eggs were bought from the local market. K₂SO₄, AgNO₃, SnCl₂·4H₂O, BaCl₂, Pb(NO₃)₂, CuSO₄·5H₂O, MgSO₄·7H₂O, MnSO₄·H₂O, FeCl₂·4H₂O, FeCl₃, ZnCl₂, NaCl,CaCl₂, L-alanine, L-lysine, L-phenylalanine, L-cysteine, L-arginine, L-glycine, L-aspartic acid, L-glutamine, glucose, fructose, urea and glutathione (GSH) were purchased from Adamas-Beta Co., Ltd. (Shanghai, China). Dopamine hydrochloride and tris(hydroxymethyl)aminomethane (Tris) were obtained from 9 Ding Chemistry Co., Ltd. (Shanghai, China). 96-well plates (In vitro scientific) were obtained from Xinyou Biotechnology Co., Ltd. (Hangzhou, China). 6-well plates were obtained from Nest Biotechnology Co., Ltd. (Wuxi, China). Black opaque 96-well plates were supported by Jet Bio-Filtration Co., Ltd. (Guangzhou, China). Dulbecco’s modified Eagle’s medium (DMEM), trypsin, penicillin, streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Sijiqing Biotechnology Co., Ltd., (Hangzhou, China). All of the chemicals were analytical grade and used without further purification.

Gelatin, L-phenylalanine (99%), L-tyrosine (99%), and naringin (98%) were purchased from Adamas-beta(Shanghai,China).Palmitic acid (>97%), stearic acid (>98%), linoleic acid (>97%), and D-(+)-raffinose(>98%) were purchased from TCI Chemical Industries (Shanghai, China).Abscisic acid (ABA, 98%) were purchased from Sigma-Aldrich(St. Louis, USA).Dipalmitoyl phosphatidylcholine (DPPC) was purchased from Avanti Polar Lipids (Alabama, USA). Indolebutyric acid (99%)and α-cyclodextrin (≥98%) were purchased from Yuanye Biotechnology (Shanghai, China).Glycerol trioleate (99%) and 2,5-dihydroxybenzoic acid (DHB, 99%)were purchased from J&K Scientific (Beijing, China).Paeoniflorin (98%), sucrose (AR), and L-glutamic acid (≥99%) were purchased from Titan Technology (Shanghai, China).Ethanol (absolute, AR grade) was purchased from Sinopharm (Beijing, China).Methanol (HPLC grade) and dichloromethane (AR grade) were purchased from Boer Chemical Reagent (Shanghai, China), deionized water was obtained from Milli-Q Water Purification System (Millipore, Billerica, MA, USA).
Tissue-Tek OCT compound was purchased from Sakura Finetek (CA, USA). Microscope cover glasses (22mm × 22mm) were purchased from Citotest Scientific (Nanjing, China).We purchased fresh sweet corn from a local supermarket. Wheat seeds (Jimai 22) were from Crop Research Institute, Shandong Academy of Agricultural Sciences.