Fresh urine was collected from all animals in a fasting state at the end of the study period and stored at 4ºC. Combur10 Test strips (Roche Diagnostic GmbH, Mannheim, Germany) used to measure Glucose, pH, and protein. Cobas u 411 urine analyzer instrument (Roche Diagnostics, Indianapolis, USA) was used to analyze urine chemistry parameters. The appearance and sedimentation were tested by visual inspection. Gravimeter (VET 360 Reichert Technologies, New York, USA) was used to check the specific gravity.
Combur10 test strips
Manufactured by Roche
Sourced in Germany
Combur10 Test strips are a diagnostic tool used to detect and measure various analytes in urine samples. The strips contain multiple reagent pads that change color when exposed to specific substances, allowing for the semi-quantitative analysis of parameters such as pH, protein, glucose, ketones, bilirubin, urobilinogen, nitrite, leukocytes, and erythrocytes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using combur10 test strips
1
Comprehensive Urine Analysis Protocol
2
Urine Analysis Workflow Validation
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Urine dipstick measurements were performed with Urisys™ 2400 (Roche Diagnostics GmbH, Mannheim, Germany) using Combur10Test™ strips. This analyzer uses the following measurement methods: reflectance photometry (wave lengths 470 nm, 555 nm, 620 nm; refractometry (density), turbidimetry (cloudiness)). Urine particle analysis was performed with UF-1000i™ (Sysmex, Norderstedt, Germany) as quantitative and semi-quantitative analysis. This instrument uses the following measurement methods: fluorescence flow cytometry with diode laser and hydrodynamic focusing and conductometry. The reference ranges were as recommended by the respective manufacturers.
Measurements were done within 90 min, 120 min and 240 min from sample collection. The measurement time point of 90 min was chosen since it represents a feasible preanalytical time period in our daily routine.
Prior to each measurement, the urine was mixed automatically as well as manually. First, urine dipstick analysis was performed for the following parameters: specific weight, pH, leucocytes, erythrocytes, nitrite, protein, glucose, ketone, urobilinogen, bilirubin. Next, automated urine particle analysis was performed for the following parameters: conductivity, white blood cell count (WBC), red blood cell count (RBC), epithelial cells (EC), bacteria (bac) and casts. For each measurement, 4 mL of urine were used.
Measurements were done within 90 min, 120 min and 240 min from sample collection. The measurement time point of 90 min was chosen since it represents a feasible preanalytical time period in our daily routine.
Prior to each measurement, the urine was mixed automatically as well as manually. First, urine dipstick analysis was performed for the following parameters: specific weight, pH, leucocytes, erythrocytes, nitrite, protein, glucose, ketone, urobilinogen, bilirubin. Next, automated urine particle analysis was performed for the following parameters: conductivity, white blood cell count (WBC), red blood cell count (RBC), epithelial cells (EC), bacteria (bac) and casts. For each measurement, 4 mL of urine were used.
3
Urine and Blood Ketone Analysis
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Patient urine samples were analyzed using Combur® 10 Test Strips (Hoffmann-La Roche, Basel, Switzerland), while capillary blood samples were analyzed using the Precision Xceed system with blood β-ketone test strips (Abott, Chicago, IL, USA). Combur® Test Strips detect AcAc (detection limit approximately 0.5 mmol/L) and Acn (detection limit approximately 7 mmol/L), while blood test strips detect βOHB (detection limit < 1 mmol/L).
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