Pegasus 4d gc gc tof ms

Manufactured by Leco

Sourced in United States, Sao Tome and Principe

The Pegasus 4D GC×GC/TOF-MS is a gas chromatography-time-of-flight mass spectrometry (GC×GC/TOF-MS) system. It combines comprehensive two-dimensional gas chromatography (GC×GC) with time-of-flight mass spectrometry (TOF-MS) technology to provide high-resolution separation and sensitive detection of complex samples.

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Lab products found in correlation

Rtx 5ms, by Restek (2 mentions) Chromatof software, by Leco (1 mentions) Rtx 200, by Restek (1 mentions) Rxi 17, by Restek (1 mentions) Research grade helium, by Airgas (1 mentions)

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Pegasus 4D (37 citations)

6 protocols using pegasus 4d gc gc tof ms

GC-MS Analysis of Metabolites

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We applied the procedure used by Hua et al. to perform GC–MS analysis [42 ]. GC–MS was performed using the Pegasus® 4D GC × GC-TOFMS from LECO (St. Joseph, MI). The separation conditions on an Rtx-5MS column (30 m × 0.25 mm × 0.25 μm) for GC–MS analysis was set as follows: column flow, 1 mL/min helium; injector temperature, 250 °C; GC temperature program: 40 °C for 1 min, increase to 300 °C in 10 °C/min changes, 300 °C for 8 min; solvent delay, 440 s; transfer line temperature, 300 °C; pegasus acquisition rate, 10 spectra/sec; mass range saved: m/z 50–800; ion source temperature: 200 °C.
The data were analyzed using the program ChromaTOF® Software from LECO (St. Joseph, MI), by setting the threshold for up to 650 hits on m/z similarity and compared with the metabolites present in libraries of the software. Compounds with peak-areas below 700 were undetectable and compounds with less than 650 hits for m/z identification were removed. The annotated synthetic compounds were removed.

Lin J.Y., Juo B.R., Yeh Y.H., Fu S.H., Chen Y.T., Chen C.L, & Wu K.P. (2021). Putative markers for the detection of early-stage bladder cancer selected by urine metabolomics. BMC Bioinformatics, 22, 305.

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GC-MS Analysis of Compounds via Kovats Index

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The samples were analyzed using LECO Pegasus 4D GC×GC−TOF MS (St Joseph, MI, USA). The first dimension capillary column was Restek Rtx-5MS (30 m × 0.25 mm × 0.25 μm) and the second capillary column was Restek Rtx-200 (2 m × 0.25 mm × 0.25 μm). The GC temperature program was set as follows: injection temperature: 280 °C; oven temperature: 40 °C maintained for 1 min, and increased at a rate of 10 °C/min to 310 °C and held constant for 8 min. The helium flow rate was set at 1 mL/min. The mass spectrometry temperature was set at 320 °C. The ion source temperature was 200 °C, and the analysis mass range was 50-800 m/z. KWM-EO was ran in hexane with a dilution of 1 mg/mL. Hexadecane solution, 64.25 μg/mL, was used as an internal standard to monitor the shift of retention time. Compounds were identified by matching the mass spectra fragmentation patterns, and the results were compared with LECO/Fiehn and Wiley Registry 9th Edition mass spectral library and NIST. Linear Kovats index of n-alkanes (C₇-C₄₀, C₇-C₃₀) were calculated for each compound and compared with the literature to identify the compound ID [30 (link)].

Chang C.T., Soo W.N., Chen Y.H, & Shyur L.F. (2019). Essential Oil of Mentha aquatica var. Kenting Water Mint Suppresses Two-Stage Skin Carcinogenesis Accelerated by BRAF Inhibitor Vemurafenib. Molecules, 24(12), 2344.

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Metabolomic Analysis of Retinal Samples

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Retinal samples were freshly harvested and homogenized ultrasonically for 4 min. Next, 3 μl 2,2,3,3-²H4-succinic acid (CDN Isotopes, Pointe-Claire, Canada) was added to each tissue sample as an internal standard in water, and an additional 200 μl water was added according to the reported protocol [7 (link)]. Briefly, 150 μl methanol and 50 μl chloroform were added and the mixture was centrifuged at 3600 ×g for 10 min. The clear supernatants were collected, and nitrogen and 80 μl methoxamine (15 mg/ml) were added to the dried samples. The samples were incubated overnight and Bis-trimethylsilyl-trifluoroacetamide (BSTFA) was added to the reaction samples. They were concentrated for 1.5 h and prepared for GC/MS analysis (Comprehensive Two-dimensional Gas Chromatography/Time-of-flight Mass Spectrometer, Pegasus 4D GC×GC-TOFMS, Leco, St. Joseph, MI).

Li T., Hu J., Du S., Chen Y., Wang S, & Wu Q. (2014). ERK1/2/COX-2/PGE2 signaling pathway mediates GPR91-dependent VEGF release in streptozotocin-induced diabetes. Molecular Vision, 20, 1109-1121.

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GC×GC/TOF-MS Analysis of Egg Samples

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Egg samples were analyzed using a Pegasus 4D GC×GC/TOF-MS (LECO, St. Joseph, MI, USA). One μL of the final extract was injected with splitless mode and with research grade helium (99.995% Airgas Wes El Cajon, CA USA) as the carrier gas. The instrument parameters are summarized in S1 Table.

Millow C.J., Mackintosh S.A., Lewison R.L., Dodder N.G, & Hoh E. (2015). Identifying Bioaccumulative Halogenated Organic Compounds Using a Nontargeted Analytical Approach: Seabirds as Sentinels. PLoS ONE, 10(5), e0127205.

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GC-MS Analysis of Blubber Samples

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Blubber sample extracts were analyzed utilizing a Pegasus 4D GC×GC/TOF-MS (LECO, St. Joseph, MI, USA). The first-dimension (1D) column was a Restek (Bellefonte, PA) Rtx-5MS (30m × 0.25mm i.d. × 0.25μm film thickness) with a 5 m guard column, and the second dimension (2D) column was a Restek Rxi-17 (1m × 0.10mm i.d.× 0.10μm film thickness). Two μL of extract was introduced into the splitless mode auto injector at 300 °C. Research grade helium (Airgas, Radnor, PA) was used as the carrier gas at 1 mL/min. The primary oven temperature started at 60 °C (1 min hold), ramped at 10 °C/min to 300 °C (3 min hold), and ramped at 20 °C/min to 320 °C (20 min hold). The secondary oven temperature was maintained 20 °C higher than the primary oven temperature. For GC×GC, the modulation period was 3.5 s with a 0.9 s hot pulse duration, and the modulator temperature offset was 35 °C relative to the primary oven temperature. The MS transfer line and ion source temperatures were 285 °C and 250 °C, respectively. The MS was operated in the electron ionization (EI) mode with a detector voltage of 1600 V, electron energy of −70 eV and data acquisition rate of 150 spectra/s.

Alonso M.B., Maruya K.A., Dodder N.G., Lailson-Brito J J.r., Azevedo A., Santos-Neto E., Torres J.P., Malm O, & Hoh E. (2017). Non-targeted screening of halogenated organic compounds in bottlenose dolphins (Tursiops truncatus) from Rio de Janeiro, Brazil.. Environmental science & technology, 51(3), 1176-1185.

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Comprehensive Analytical Workflow for Water Samples

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Samples were analyzed using Pegasus 4D GC×GC/TOF-MS (LECO, St.
Joesph, MI); detailed instrumental conditions are listed in Table S4. Data processing was conducted using
the ChromaTOF software (version 4.72.0), yielding a list of features
with distinct chromatographic peaks and associated fragmentation mass
spectra. The total number of chromatographic features detected per
treatment cycle is listed in Table S5.
Peak area abundances for all chromatographic features were normalized
by dividing each of the chromatographic feature’s peak area
by the volume of sample extracted (influent, 500 mL; AeMP and AnMP,
1000 mL each). Then, the average total number of chromatographic features
and the average total peak area abundances per sample across the experiments
(influent, n = 3; AeMP, n = 3; AnMP, n = 2) were each evaluated using an analysis of variance
(ANOVA) followed by a Bonferroni adjusted post hoc test (Tables S6–S9). The ANOVA and post hoc
tests were conducted by using IBM SPSS software (version 27).

Johnson J.L., Dodder N.G., Mladenov N., Steinberg L., Richardot W.H, & Hoh E. (2024). Comparison of Trace Organic Chemical Removal Efficiencies between Aerobic and Anaerobic Membrane Bioreactors Treating Municipal Wastewater. ACS Es&t Water, 4(4), 1381-1392.

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