200 imaging scanner

Manufactured by Bioscan

Sourced in United States

The Bioscan 200 is an imaging scanner designed for laboratory use. It is a compact and versatile device that can capture high-quality images of various samples. The scanner features a digital imaging sensor and supports multiple scanning modes to accommodate different sample types and applications. The Bioscan 200 is intended to provide reliable and consistent imaging results for laboratory professionals.

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Lab products found in correlation

Gamma 8000 counter, by Beckman Coulter (3 mentions) Kinetex, by Phenomenex (3 mentions) 2414 differential refractometer, by Waters Corporation (2 mentions) Chromatograph, by Waters Corporation (2 mentions) Kta fplc system, by GE Healthcare (2 mentions) 2695 separation module, by Malvern Panalytical (2 mentions) Visco gel 1 series columns, by Malvern Panalytical (2 mentions) Pall itlc sg plates, by Avantor (2 mentions) Beckman 170 radioisotope detector, by Beckman Coulter (2 mentions) Dynapro nanostar, by Wyatt Technology (2 mentions) Nai crystal, by Beckman Coulter (2 mentions) Vxr 500, by Agilent Technologies (1 mentions) Mercury 300 spectrometer, by Agilent Technologies (1 mentions) Unity inova 400 spectrometer, by Agilent Technologies (1 mentions) Bxpc 3 cells, by American Type Culture Collection (1 mentions)

6 protocols using 200 imaging scanner

Radiolabeling Efficiency Evaluation

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All solvents and reagents used in this
study were obtained from Sigma Aldrich (St. Louis, MO) or Fisher Scientific
(Pittsburgh, PA). Buffers used for radiolabeling were prepared in
chelexed Milli-Q water which was filtered through a 0.22 μm
nylon filter. A Varian Mercury-300 spectrometer or a VARIAN UNITY
Inova 400 spectrometer was utilized to record ¹H (300 MHz)
NMR spectra. A VARIAN VXR 500 with a UNITY INOVA Console spectrometer
was used to record ¹³C (126 MHz) NMR spectra. Chemical
shifts are reported in parts per million and referenced to residual
solvent resonance peaks. Radio-TLC detection was accomplished using
a Bioscan 200 imaging scanner (Bioscan, Inc., Washington, DC). Radioactive
samples were counted on a Beckman Gamma 8000 counter containing a
NaI crystal (Beckman Instruments, Inc., Irvine, CA). A two-solvent
reversed-phase HPLC system was used to evaluate the radiolabeling
efficiency with water [0.05% trifluoroacetic acid (TFA)] and acetonitrile
(0.05% TFA). HPLC used a Kinetex (Phenomenex) C-18 column (5 μm,
4.6 × 150 mm I.D.). The HPLC instrument was composed of UV absorbance
detectors set at 220 and 280 nm, a NaI radiotracer detector, and a
photodiode array detector. A gradient elution with acetonitrile (0.1%
TFA) 0–100% buffer mobile phase over the course of 13 min and
a 1 mL/min flow rate was developed for radiochemical purity profiling.

Huynh T.T., Wang Y., Terpstra K., Cho H.J., Mirica L.M, & Rogers B.E. (2022). 68Ga-Labeled Benzothiazole Derivatives for Imaging Aβ Plaques in Cerebral Amyloid Angiopathy. ACS Omega, 7(23), 20339-20346.

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Characterization of Polymer Nanoparticles

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Gel permeation chromatography (GPC) was carried out on a Waters (Millford, MA) chromatograph equipped with a Waters Alliance high pressure liquid chromatography (HPLC) system pump (2695 Separation Module) and four Visco Gel I-Series columns from Viscotek (dimensions = 7.8 mm × 30 cm). Detection was provided by a Waters 2414 differential refractometer and N,N-dimethyl formamide (DMF) with 0.1 % LiBr used as the mobile phase. Copolymer chromatograms were run at room temperature and calibrated to poly(methyl methacrylate) (PMMA) standards. Dynamic light scattering (DLS) was performed on a Wyatt Technology (Goleta, CA) DynaPro NanoStar™ at room temperature. Data was collected on 0.1 wt% aqueous nanoparticle solutions filtered through a 0.2 μm filter. Zeta potential measurements were acquired on a Malvern Zetasizer (Zetasizer Nano ZS ZEN3600, Westborough, MA). A Bioscan 200 imaging scanner (Bioscan, Washington, DC) was used to read the instant thin layer chromatography (ITLC) plates (Pall ITLC-SG plates, VWR International, Batavia, IL). Fast protein liquid chromatography (FPLC) and radio-FPLC were performed using an ÄKTA FPLC system (GE Healthcare Biosciences, Pittsburgh, PA) equipped with a Beckman 170 Radioisotope Detector (Beckman Instruments, Fullerton, CA).

Liu Y., Luehmann H.P., Detering L., Pressly E.D., McGrath A.J., Sultan D., Nguyen A., Grathwohl S., Shokeen M., Zayed M., Gropler R.J., Abendschein D., Hawker C.J, & Woodard P.K. (2019). Assessment of Targeted Nanoparticle Assemblies for Atherosclerosis Imaging with Positron Emission Tomography and Potential for Clinical Translation. ACS applied materials & interfaces, 11(17), 15316-15321.

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Radiolabeled Peptide Synthesis and Characterization

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All solvents and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Fisher Scientific (Pittsburgh, PA, USA) and used as received. All solutions and buffers were prepared using HPLC-grade water. Radio-TLCs employed Whatman 60 Å silica gel thin-layer chromatography (TLC) plates and were analyzed using a Bioscan 200 imaging scanner (Bioscan, Inc., Washington, DC, USA). Radioactivity was counted with a Beckman Gamma 8000 counter containing a NaI crystal (Beckman Instruments, Inc., Irvine, CA, USA). The peptides EB-DOTA-(PEG28)₂-A20FMDV2, IBA-DOTA-(PEG28)₂-A20FMDV2, and non-albumin peptide DOTA-(PEG28)₂-A20FMDV2 were synthesized by AnaSpec company (Fremont, CA, USA) and characterized by HPLC and mass spectrometry. A stock solution of 1 nmol/μL was made with HPLC-grade water and stored at −20 °C before use. Non-PEGylated A20FMDV2 peptide served as a blocking agent. BxPC-3 cells were purchased from ATCC (Manassas, VA, USA) and grown in RPMI 1640, 10% FBS, and 10 mM HEPES. The cells were cultured in an incubator at 37 °C, 5% CO2, and harvested in PBS by trypsin-EDTA 0.25% before use.

Huynh T.T., Sreekumar S., Mpoy C, & Rogers B.E. (2022). Therapeutic Efficacy of 177Lu-Labeled A20FMDV2 Peptides Targeting ανβ6. Pharmaceuticals, 15(2), 229.

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Characterization of Polymer Nanoparticles

Cited 1 time

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Gel permeation chromatography (GPC) was carried out on a Waters (Millford, MA) chromatograph equipped with a Waters Alliance high pressure liquid chromatography (HPLC) system pump (2695 Separation Module) and four Visco Gel I-Series columns from Viscotek (dimensions = 7.8 mm × 30 cm). Detection was provided by a Waters 2414 differential refractometer and dimethyl formamide with 0.1 % LiBr was used as the mobile phase. Copolymer chromatograms were run at room temperature and calibrated to poly(methyl methacrylate) (PMMA) standards. Dynamic light scattering (DLS) was performed on a Wyatt Technology (Goleta, CA) DynaPro NanoStar™ at room temperature. Data was collected on 0.1 wt% aqueous nanoparticle solutions filtered through a 0.2 μm filter. Zeta potential measurements were acquired on a Malvern Zetasizer (Zetasizer Nano ZS ZEN3600). A Bioscan 200 imaging scanner (Bioscan, Washington, DC) was used to read the instant thin layer chromatography (ITLC) plates (Pall ITLC-SG plates, VWR International, Batavia, IL). Fast protein liquid chromatography (FPLC) and radio-FPLC were performed using an ÄKTA FPLC system (GE Healthcare Biosciences) equipped with a Beckman 170 Radioisotope Detector (Beckman Instruments, Fullerton, CA). All other instrumentation can be found in our previous report (31 (link)).

Woodard P.K., Liu Y., Pressly E.D., Luehmann H.P., Detering L., Sultan D., Laforest R., McGrath A.J., Gropler R.J, & Hawker C.J. (2016). Design and Modular Construction of A Polymeric Nanoparticle for Targeted Atherosclerosis Positron Emission Tomography Imaging: A Story of 25% 64Cu-CANF-Comb. Pharmaceutical research, 33(10), 2400-2410.

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Radiolabeling and Characterization of SAR-BBN

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All solvents and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Fisher Scientific (Pittsburgh, PA, USA) and used as received unless stated otherwise. All solutions and buffers were prepared using HPLC-grade water. Radio-TLCs employed Whatman 60 Å silica gel thin-layer chromatography (TLC) plates and were analyzed using a Bioscan 200 imaging scanner (Bioscan, Inc., Washington, DC, USA). Radioactivity was counted with a Beckman Gamma 8000 counter containing a NaI crystal (Beckman Instruments, Inc., Irvine, CA, USA). Reversed-phase high-pressure liquid chromatography (HPLC) was used to evaluate the radiolabeling efficiency. HPLC utilizes a two-solvent system: water (0.05% trifluoroacetic acid (TFA)) and acetonitrile (0.05% TFA). The system was equipped with UV absorbance detectors (UV, 220 nm and 280 nm), a NaI radiotracer detector, and a photodiode array detector. HPLC analysis of peptides used Kinetex (Phenomenex, Torrance, CA, USA) C-18 column (5 μm, 4.6 × 150 mm I.D.).
SAR-BBN was synthesized and characterized by Auspep Pty Ltd. (Melbourne, Australia). SAR-BBN was diluted in HPLC-grade water to make a stock solution of 1 nmol/10 μL. The bombesin analog Tyr⁴-BBN (pGlu-Gln-Arg-Tyr-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH₂) was used as a blocking agent and purchased from Sigma-Aldrich (St. Louis, MO, USA).

Huynh T.T., van Dam E.M., Sreekumar S., Mpoy C., Blyth B.J., Muntz F., Harris M.J, & Rogers B.E. (2022). Copper-67-Labeled Bombesin Peptide for Targeted Radionuclide Therapy of Prostate Cancer. Pharmaceuticals, 15(6), 728.

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Radiolabeling and Characterization of Peptides

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All solvents and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Fisher Scientific (Pittsburgh, PA, USA) and used as received unless stated otherwise. All solutions and buffers were prepared using HPLC-grade water. Peptides were custom synthesized and characterized by AnaSpec (Fremont, CA, USA). Stock solutions of the peptides (1 nmol/μl) were prepared in HPLC-grade water and stored at −20^°C before use. Non-PEGylated A20FMDV2 peptide served as blocking agent. Radio-TLCs employed Whatman 60 Å silica gel thin-layer chromatography (TLC) plates and were analyzed using a Bioscan 200 imaging scanner (Bioscan, Inc., Washington, DC, USA). Reversed-phase high-pressure liquid chromatography (HPLC) was used to evaluate the radiolabeling efficiency. HPLC utilized a two-solvent system: water (0.05% trifluoroacetic acid (TFA)) and acetonitrile (0.05% TFA). The system was equipped with UV absorbance detectors (UV, 220 and 280 nm), a NaI radiotracer detector and a photodiode array detector. HPLC analysis of peptides used Kinetex (Phenomenex) C-18 column (5 μm, 4.6 × 150 mm I.D.).

Huynh T.T., Sreekumar S., Mpoy C, & Rogers B.E. (2022). A comparison of 64Cu-labeled bi-terminally PEGylated A20FMDV2 peptides targeting integrin ανβ6. Oncotarget, 13, 360-372.

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