Skin samples were preserved in methanol/dimethyl sulfoxide (DMSO) (4:1), dehydrated, and embedded in paraffin. Posteriorly, they were cut by microtome into (5 μm) sections, deposited on a slide, and stained with hematoxylin–eosin (HE) for quantitative and differential analysis of the cell recruitment profile. The slide was visualized by the 40× objective and 20 random images (total area covered 1.5 × 106 μm2) were digitized using a microcamera Leica DM5000B attached to a microscope and Leica Application Suite program (version 2.4.0 R1 Leica Microsystems-Switzerland-Ltd, Heerbrugg, Switzerland). To quantify inflammatory cell infiltrates in the dermis/epidermis the images were analyzed using the Leica QWin V3 program (Leica Microsystems-Switzerland-Ltd, Heerbrugg, Switzerland) for counting all cell nuclei, excluding hair follicles from the analysis. The results are expressed by the number of cells per 75,256.2 mm2. To identify the cell recruitment profile to the inoculum site, we performed a differential leukocyte count (mononuclear or polymorphonuclear) using optical microscopy. The results are expressed by the percentage values of cells infiltrating differential leukocyte count and displayed on pie charts.
Qwin v3 program
Manufactured by Leica
Sourced in Switzerland
The Leica QWin V3 program is a software application designed for image analysis and quantification of microscopic samples. It provides a platform for acquiring, processing, and analyzing digital images from various Leica microscope systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using qwin v3 program
1
Quantitative Analysis of Inflammatory Cell Infiltrates
2
Collagen Fibers and GFAP Analysis
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Collagen fibers’ area% and GFAP +ve cell count in all groups were measured in image analysis system (Leica Qwin V3 program [Leica Microsystems] Switzerland. LTD) in Research Central Laboratory, Faculty of Medicine, Tanta, Egypt. All data (collagen fibers area%, GFAP +ve cell count, TC, TGs, and HDL-c) for all groups were expressed as mean ± standard deviation (SD) statistical analyses were carried out using SPSS software (SPSS Science, version 11.0.1, Chicago, Illinois, USA). The post hoc test was used to compare between groups. All data were expressed as means ± SD, P ≤ 0.05, and 0.001 were considered significant and highly significant, respectively.
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