Microflex lrf spectrometer

Manufactured by Bruker

The Microflex LRF spectrometer is a compact and robust lab equipment designed for matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. It provides high-performance and reliable data acquisition capabilities for a wide range of applications.

Automatically generated - may contain errors

Lab products found in correlation

Jsx 3100rii, by JEOL (1 mentions) Ecz 400, by JEOL (1 mentions)

Close spelling variants of this product

Microflex LRF MALDI-TOF mass spectrometer Microflex LRF mass spectrometer Microflex LRF Microflex spectrometer Microflex Bruker spectrometers

2 protocols using microflex lrf spectrometer

Comprehensive Nanomaterial Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Elemental analyses and electrospray-ionisation mass spectrometry (ESI-MS) were conducted at the analysis centre of Hokkaido University. The ¹H NMR and ¹H-¹H COSY NMR spectra were acquired on a JEOL ECZ-400S or EX-270 spectrometer. Energy dispersive XRF spectra were acquired on a JEOL JSX-3100RII spectrometer using a Rh target. DLS analyses were conducted using an OTSUKA ELSZ-1000SCl analyser. Nitrogen and methanol vapour adsorption isotherms were measured using an automatic BELSORP-max (MicrotracBEL Co.) volumetric adsorption apparatus. Pore-size distributions were calculated using the density functional theory (DFT) method (DFT kernel: N₂ at 77 K on silica, cylindrical pores, and NLDFT equilibrium model). Thermogravimetry-differential thermal analysis (TG-DTA) measurements were recorded using a Rigaku Thermoplus EVO TG-DTA 8120 with Al sample pans under an Ar flow. Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) was conducted using a Bruker Microflex LRF spectrometer in a linear mode.

Matsukawa H., Yoshida M., Tsunenari T., Nozawa S., Sato-Tomita A., Maegawa Y., Inagaki S., Kobayashi A, & Kato M. (2019). Fast and stable vapochromic response induced through nanocrystal formation of a luminescent platinum(II) complex on periodic mesoporous organosilica. Scientific Reports, 9, 15151.

+ Open protocol

+ Expand

Mapping TEFM Binding Site via Cross-Linking

Check if the same lab product or an alternative is used in the 5 most similar protocols

To map the TEFM binding site, EC-TEFM complex (5 μM in 40 mM Na-HEPES pH 7.5) was treated with a mixture of DSG/[²H₄]-DSG (0.6 mM DMSO solution, ProteoChem) and the products of the reaction separated using 7% Tris-glycine SDS PAGE. The cross-linked species were excised from the gel and subjected to trypsin digestion in 100 mM NH₄HCO₃ buffer overnight at 37°C. The products of the digestion r eaction were extracted from the gel with CH₃CN-0.1% TFA in water (9:1), dried in vacuum, re-dissolved in 10 mg/ml CHCA matrix in CH₃CN-0.1% TFA in water (1:1), and the resulting solution applied on target for MALDI-TOF mass spectrometry. Mass spectra were taken on a MicroFlex LRF spectrometer (Bruker). Positively charged ions (M+H⁺) were analyzed in the reflector mode (m/z 1000 to 4000) and cross-linked peptides identified using xBobcat mass matching search engine (http://prottools.ethz.ch/orinner/public/htdocs/xquest/index_review.html) (Rinner et al., 2008 (link)).

Hillen H.S., Parshin A.V., Agaronyan K., Morozov Y.I., Graber J.J., Chernev A., Schwinghammer K., Urlaub H., Anikin M., Cramer P, & Temiakov D. (2017). Mechanism of transcription anti-termination in human mitochondria. Cell, 171(5), 1082-1093.e13.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!