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Ab109110

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Ab109110 is a lab equipment product. It is a device used in scientific research and laboratory applications.

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Lab products found in correlation

Ab150080, by Abcam (2 mentions) Ab126712, by Abcam (2 mentions) Biotracker nir 633, by Merck Group (2 mentions) Sp5 x mp confocal microscope, by Leica (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Ab6718, by Abcam (1 mentions) Irdye 800cw, by LI COR (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Ab134953, by Abcam (1 mentions) Ab124767, by Abcam (1 mentions) Ab56416, by Abcam (1 mentions) Alexa fluor 633, by Thermo Fisher Scientific (1 mentions)

5 protocols using ab109110

1

HPV L1 Antibody Neutralization Controls

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The HPV16 L1 antibody H16.V5, HPV18 L1 antibody H18.G10, and HPV31 L1 antibody H31.A6, used as neutralization controls within hrHPV infection studies, were gifts from Neil Christensen (The Pennsylvania State University). For western blot analysis of HPV surface binding the HPV16 L1 mAb CAMVIR-1 (550840, BD Bioscience), β-actin (4970, Cell Signaling Technologies), goat-anti-mouse IRDye 800CW (925-322, LI-COR), and goat-anti-rabbit (H + L) Alexa Fluor 680 (A27042, Thermo Fisher) were used. Immunofluorescent imaging was carried out using the HPV16 L1 antibody 56E.L1 (a kind gift from Martin Sapp, Louisiana State University), early endosomal antigen 1 (EEA-1) (Ab109110, Abcam), rabbit isotype control (02-6102, Thermo Fisher), mouse isotype control (03001D, BD Biosciences), TRITC goat-anti-rabbit (ab6718, Abcam), and DyLight 488 goat anti-mouse (405310, Biolegend).
Skeate J.G., Segerink W.H., Garcia M.D., Fernandez D.J., Prins R., Lühen K.P., Voss F.O., Da Silva D.M, & Kast W.M. (2020). Theta-Defensins Inhibit High-Risk Human Papillomavirus Infection Through Charge-Driven Capsid Clustering. Frontiers in Immunology, 11, 561843.
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2

Intracellular Trafficking of F-AuNSs

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Cells (5000 cells per well) were seeded in 8-well chamber slides (Lab-Tek) and incubated overnight. Cells were then exposed to F-AuNSs (100 μg per mL) at different time points (2, 4, 6, 8, 12, 18, and 24 h), fixed with 4% formaldehyde solution for 10 min, permeabilized with 0.1% Triton X-100 for 1 h, and blocked with 10% normal goat serum solution at room temperature for 1 h. Cells were incubated overnight at 4 °C with anti-EEA1 antibody (Abcam, ab109110, 1/250) for early endosome staining or anti-RAB7 antibody (Abcam, ab126712, 1/50) for late endosome staining, and washed three times followed by staining with Alexa Fluor 594-conjugated anti-rabbit IgG (Abcam, ab150080, 1/500). For lysosome imaging, cells were stained with BioTracker NIR 633 (Sigma, 1/500) according to the manufacturer’s instructions. Slides were mounted with DAPI nuclear dye and visualized under a Leica SP5 X MP confocal microscope. Images were captured utilizing a 63× objective.
Chen M.S., Tung K.S., Coonrod S.A., Takahashi Y., Bigler D., Chang A., Yamashita Y., Kincade P.W., Herr J.C, & White J.M. (1999). Role of the integrin-associated protein CD9 in binding between sperm ADAM 2 and the egg integrin α6β1: Implications for murine fertilization. Proceedings of the National Academy of Sciences of the United States of America, 96(21), 11830-11835.
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3

Immunofluorescence Staining of Cellular Markers

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Coverslip were incubated for 15 min in PBS with 4% paraformaldehyde, washed with PBS, and incubated in PBS plus 0.5% Triton X‐100 during 10 min. The cells were washed three times with PBS, and the coverslips were incubated during 1 h with primary antibody against ubiquitin (ab134953, Abcam), EEA1 (ab109110, Abcam), M6PR (ab124767, Abcam), and P62/Sqstm1 (ab56416, Abcam). After washing with PBS, the coverslips were incubated with a goat anti‐mouse or anti‐rabbit secondary antibody conjugated with Alexa 488 or CY3 (Interchim SA) for 1 h, washed twice with PBS, and incubated for 3 min in PBS/DAPI (1/10,000 dilution). Coverslips were rinsed twice before mounting in Pro‐Long media (Molecular Probes) and were examined using a Leica microscope.
Boivin M., Pfister V., Gaucherot A., Ruffenach F., Negroni L., Sellier C, & Charlet‐Berguerand N. (2020). Reduced autophagy upon C9ORF72 loss synergizes with dipeptide repeat protein toxicity in G4C2 repeat expansion disorders. The EMBO Journal, 39(4), e100574.
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4

Immunofluorescence Assay for SARS-CoV-2 Proteins

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Antibodies targeting SARS-CoV-2 nucleocapsid (SinoBiological, #40143-MM05), EEA-1(Abcam, #ab109110), anti-LAMP1 (Abcam, #ab24170,), Rab7 (Origene, #AB0033-200). Goat anti-Mouse IgG (H+L), cross-absorbed secondary antibody, Alexa Fluor™ 633 (Thermo Scientific # A-21052A-21050), Goat anti-rabbit IgG (H+L), cross-absorbed secondary antibody, Alexa Fluor™ 488 (Thermo Scientific #A-11008).
Karim M., Mishra M., Lo C.W., Saul S., Cagirici H.B., Tran D.H., Agrawal A., Ghita L., Ojha A., East M.P., Gammeltoft K.A., Sahoo M.K., Johnson G.L., Das S., Jochmans D., Cohen C.A., Gottwein J., Dye J., Neff N., Pinsky B.A., Laitinen T., Pantsar T., Poso A., Zanini F., Jonghe S.D., Asquith C.R, & Einav S. (2024). PIP4K2C inhibition reverses autophagic flux impairment induced by SARS-CoV-2. bioRxiv.
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5

Intracellular Trafficking of F-AuNSs

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Cells (5000 cells per well) were seeded in 8-well chamber slides (Lab-Tek) and incubated overnight. Cells were then exposed to F-AuNSs (100 μg per mL) at different time points (2, 4, 6, 8, 12, 18, and 24 h), fixed with 4% formaldehyde solution for 10 min, permeabilized with 0.1% Triton X-100 for 1 h, and blocked with 10% normal goat serum solution at room temperature for 1 h. Cells were incubated overnight at 4 °C with anti-EEA1 antibody (Abcam, ab109110, 1/250) for early endosome staining or anti-RAB7 antibody (Abcam, ab126712, 1/50) for late endosome staining, and washed three times followed by staining with Alexa Fluor 594-conjugated anti-rabbit IgG (Abcam, ab150080, 1/500). For lysosome imaging, cells were stained with BioTracker NIR 633 (Sigma, 1/500) according to the manufacturer’s instructions. Slides were mounted with DAPI nuclear dye and visualized under a Leica SP5 X MP confocal microscope. Images were captured utilizing a 63× objective.
Wen X., Ou L., Cutshaw G., Uthaman S., Ou Y.C., Zhu T., Szakas S., Carney B., Houghton J., Gundlach-Graham A., Rafat M., Yang K, & Bardhan R. (2023). Physicochemical Properties and Route of Systemic Delivery Control the In Vivo Dynamics and Breakdown of Radiolabeled Gold Nanostars. Small (Weinheim an der Bergstrasse, Germany), 19(29), e2204293.
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