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Triglyceride quantification colorimetric kit

Manufactured by Merck Group
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The Triglyceride Quantification Colorimetric Kit is a laboratory product designed to quantify triglyceride levels in various sample types. The kit utilizes a colorimetric method to measure the concentration of triglycerides present in the sample.

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Lab products found in correlation

Nonidet p40 substitute, by Merck Group (1 mentions)

Close spelling variants of this product

Triglyceride Quantification Kit (24 citations) Triglyceride Quantification Colorimetric/Fluorometric Kit (9 citations)

5 protocols using triglyceride quantification colorimetric kit

1

Triglyceride Quantification Protocol

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Serum and liver triglycerides were measured by using the Triglyceride Quantification Colorimetric kit, following manufacturer’s instructions (Sigma).
Lin Y.H., Luck H., Khan S., Schneeberger P.H., Tsai S., Clemente-Casares X., Lei H., Leu Y.L., Chan Y.T., Chen H.Y., Yang S.H., Coburn B., Winer S, & Winer D.A. (2019). Aryl hydrocarbon receptor agonist indigo protects against obesity-related insulin resistance through modulation of intestinal and metabolic tissue immunity. International Journal of Obesity (2005), 43(12), 2407-2421.
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2

Adipose Tissue Triglyceride Quantification

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Tissue triglyceride was measured with Triglyceride Quantification Colorimetric kit (Sigma-Aldrich, MAK266-1KT). Tissues were dissected, and homogenized in 5% NP-40 Substitute buffer. Triglyceride was measured according to the manufacturer’s instruction. Triglyceride level was normalized to weight of adipose tissues.
Liu W., Zhou H., Wang H., Zhang Q., Zhang R., Willard B., Liu C., Kang Z., Li X, & Li X. (2022). IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nature Communications, 13, 2748.
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3

Hepatic Triglyceride Quantification

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Liver tissue (100 mg) of each mouse were homogenized in 1 mL solution of Nonidet P40 at 5% (A1694, 0250, Panreac Applichem, Spain). The amount of TG was determined by using the Triglyceride Quantification Colorimetric Kit (MAK266, Sigma Aldrich, St. Louis, MO, USA).
Sanz-Lamora H., Marrero P.F., Haro D, & Relat J. (2022). A Mixture of Pure, Isolated Polyphenols Worsens the Insulin Resistance and Induces Kidney and Liver Fibrosis Markers in Diet-Induced Obese Mice. Antioxidants, 11(1), 120.
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4

Triglyceride Quantification in Embryos

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The concentration of TGs in embryos was measured using the Triglyceride Quantification Colorimetric Kit from Sigma (Cat. #MAK266). 50 stage5 embryos were homogenized in Eppendorf tubes on a Nonidet P40 Substitute (Sigma, Cat. #74385) 5% solution. The triglycerides concentration in each homogenate was then quantified following the instructions provided by the manufacturer. Absorbance was measured at 570 nm.
Li X.C., Gandara L., Ekelöf M., Richter K., Alexandrov T, & Crocker J. (2024). Rapid response of fly populations to gene dosage across development and generations. Nature Communications, 15, 4551.
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5

Triglyceride Quantification in Mouse Liver

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Liver tissue (100 mg) of each mouse was homogenized in a 1 mL solution of 5% Nonidet P40 (A1694,0250, PanReac AppliChem, Barcelona, Spain) with ultrapure water. The amount of triglyceride (TG) was determined using the Triglyceride Quantification Colorimetric Kit (MAK266, Sigma Aldrich, St. Louis, MI, USA).
Sandoval V., Sanz-Lamora H., Marrero P.F., Relat J, & Haro D. (2021). Lyophilized Maqui (Aristotelia chilensis) Berry Administration Suppresses High-Fat Diet-Induced Liver Lipogenesis through the Induction of the Nuclear Corepressor SMILE. Antioxidants, 10(5), 637.
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