The cell lines (MDA-MB-231, MDA-MB-468, MDA-MB-436, MCF-10A, MCF-7, BT-474, and SK-BR3) were acquired from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and Procell (Wuhan, China). All cell lines except for MDA-MB-468 were cultured in a humidified incubator at 37°C with 5% CO2. MDA-MB-231 and MDA-MB-436 cells were grown in DMEM supplemented with 10% FBS (Procell, Wuhan, China) and 1% penicillin/streptomycin (Yeasen, Shanghai, China). MCF-10A, BT-474, and SK-BR3 cells were grown in appropriate special complete medium. MCF-7 cells were cultured in 1640 medium enriched with 10% FBS (Procell) and 1% penicillin/streptomycin (Yeasen). MDA-MB-468 cells were cultured in L-15 medium with 10% FBS (Procell) and 1% penicillin/streptomycin (Yeasen) in a humidified incubator at 37°C without CO2.
Mda mb 468
Manufactured by Procell
Sourced in China
MDA-MB-468 is a human breast cancer cell line. It is a commonly used model in cancer research for studying the biology and treatment of triple-negative breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231, by Procell (17 mentions)
Mcf 10a, by Procell (11 mentions)
Mcf 7, by Procell (10 mentions)
Bt 549, by Procell (8 mentions)
Skbr3, by Procell (5 mentions)
Mda mb 468, by Thermo Fisher Scientific (4 mentions)
Mda mb 231, by Thermo Fisher Scientific (3 mentions)
Zr 75 1, by Procell (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Mcf 10a, by American Type Culture Collection (2 mentions)
Mda mb 453, by Procell (2 mentions)
Mcf 7, by Thermo Fisher Scientific (2 mentions)
Cm 0525, by Procell (2 mentions)
Bt 549, by Thermo Fisher Scientific (2 mentions)
Hs578t, by Procell (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions)
Leibovitz s l 15, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Procell (1 mentions)
23 protocols using mda mb 468
1
Culturing Diverse Breast Cell Lines
2
Culturing TNBC and Non-TNBC Breast Cell Lines
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Human TNBC cell lines (MDA-MB-468 and MDA-MB-453), a non-TNBC MCF-7 cell line and a normal breast epithelial MCF-10A cell line were purchased from Procell Life Science & Technology Co., Ltd. MDA-MB-468 and MDA-MB-453 cells were cultured with the Leibovitz's L-15 medium (cat. no. CM10045; Beijing Zhongke Maichen Technology Co., Ltd.) containing 10% fetal bovine serum (cat. no. 04-001-1A; Biological Industries) and 1% penicillin-streptomycin (cat. no. P1400-100ML; Beijing Solarbio Science & Technology Co., Ltd.) at 37°C without CO2. MCF-7 and MCF-10A cells were cultured with DMEM (cat. no. C11995500BT; Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (cat. no. 04-001-1A; BioInd, Israel) and 1% penicillin-streptomycin (cat. no. P1400-100ML, Solarbio, Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO2 incubator. The cells were passaged every 4–5 days using 0.25% Trypsin-EDTA (v/v) (cat. no. 25200-056; Gibco; Thermo Fisher Scientific, Inc.).
3
Breast Cancer Cell Line Culture Protocols
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Breast cancer cell lines BT-549, MDA-MB-231, MDA-MB-468, and MCF-7 as well as an immortalized mammary epithelial-like cell line, MCF-10A were obtained from Procell biological company (Shanghai, China). BT-549 and MCF-7 cells were cultured in RPMI-1640 (Procell) supplemented with 10% (v/v) fetal bovine serum (FBS), 0.01 mg/mL insulin (Procell), 2 mM l -glutamine, 0.1 mg/mL streptomycin, and 100 U/mL penicillin at 37 °C in a humidified atmosphere containing 5% CO2. MDA-MB-231 and MDA-MB-468 cells were cultured in Leibovitz's L-15 (Gibco) with 10% (v/v) FBS, 2 mM l -glutamine, 0.1 mg/mL streptomycin, and 100 U/mL penicillin at 37 °C in a standard humidity incubator. MCF-10A was cultured in DMEM (Procell) supplemented with 5% horse serum, 20 ng/mL epidermal growth factor, 0.5 μg/mL hydrocortisone, 0.1 mg/mL streptomycin, 100 U/mL penicillin, and 0.01 mg/mL insulin at 37 °C in a humified atmosphere containing 5% CO2.
4
Cultivation of Breast Cancer Cell Lines
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Human breast cancer cell lines ZR-75-1, MCF7, JIMT1, MDA-MB-468, MDA-MB-231 and SKBR3 were purchased from Procell (https://www.procell.com.cn
5
Cell Culture Protocol for Cancer Cell Lines
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The MCF-10A, MDA-MB-231, MDA-MB-468, and MCF-7 cells were purchased from Wuhan Procell Life Science & Technology Co., Ltd. (Wuhan, China). For the cell culture, all cells were cultured with Dulbecco's Modified Eagle Medium (Shanghai Hengfei Biotechnology Co., Ltd., Shanghai, China) containing 10% fetal bovine serum (Jiangsu Kewei Biotechnology Co., Ltd., Jiangsu, China) in an incubator with 37°C and 5% CO2.
6
Culturing Human Breast Cell Lines
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The human breast epithelial cell line MCF-10A and cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-468, SK-BR-3 and HCC70) were purchased from Procell Life Science&Technology Co., Ltd. MCF-10A was grown in MCF-10A cell special medium (CM-0525, Procell, Wuhan, China). Those breast cancer cells were grown in DMEM medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum, 100 ug/mL streptomycin and 100 U/mL penicillin at 37°C with an atmosphere of 5% CO2 and 95% humidity.
7
Culture of Human Breast Cancer Cell Lines
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Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, BT549, MCF-7, and T-47D) were obtained from Procell (Wuhan, China). Cells were maintained in DMEM and RPMI 1640 supplemented with 10% FBS and 1% penicillin-streptomycin (100 U/mL penicillin and 100 mg/mL streptomycin) in an incubator with a 5% CO2 humidified atmosphere at 37 °C.
8
Culturing MDA-MB-231 and MDA-MB-468 Cells
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MDA-MB-231 and MDA-MB-468 cell lines were acquired from Procell Life Science & Technology Co., Ltd. (Wuhan, Hubei, China) and were validated by analysis of STR (PowerPlex 18D system). The cell lines were cultured in high-glucose Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Pan Biotech, Germany) at 37°C under an atmosphere of 5% CO2.
9
Culturing Human Breast Cell Lines
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Human breast immortalized cell line MCF-10A and BC cell lines MCF-7, MDA-MB-231 and MDA-MB-468 were provided by ATCC (Manassas, VA, USA) or Procell Bio (Wuhan, China). The culture medium was listed as follow: MCF-10A (DMEM:F12 = 1:1, PM150312, Procell), MCF-7 (MEM, PM150410, Procell), MDA-MB-231 and MDA-MB-468 (Leibovitz's L-15, PM151010, Procell). The medium was supplied with 10% of fetal bovine serum (FBS, FND500, ExCell Bio, Suzhou, China) and 1% Penicillin–Streptomycin Solution (PB180120, Procell). The cells were kept in a cell incubator with 37 °C and 5% CO2.
10
Cell Culture Conditions for Cancer Cell Lines
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BT-474 (originally isolated from a solid, invasive ductal carcinoma of the breast) and mammary epithelial cell line (MCF 10A) were procured from ATCC. MDA-MB-231 (derived from metastatic site: Pleural effusion) and MDA-MB-468 (derived from metastatic site: Pleural effusion) were provided by Procell. Human embryonic kidney 293T cells were procured from National Institutes for Food and Drug Control. RPMI1640 (w/o Hepes) with 10% FBS was applied for culturing BT-474. MCF 10A cells were kept in minimum essential basal medium (MEBM) with addition of 100 ng/mL cholera toxin. MDA-MB-231 and MDA-MB-468 cells were cultured in Leibovitz's L-15þ10% FBSþ1% pen-strep. 293T cells were kept in RPMI1640 medium added with 10% FBS. All cells were incubated in a humid atmosphere at 37 C with 5% CO 2 . In addition, p65 expression vector (GenePharma) and SAG (ab142160; Abcam) were respectively added to culture medium for 48 hours to activate NFkB and Hedgehog signaling pathways. Cells authentication was completed with short tandem repeat. PCR amplification was utilized for Mycoplasma testing. The passage number of all cell lines was 3.
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