Anti cd150 tc15 12f12.2

Manufactured by BioLegend

Anti-CD150 (TC15-12F12.2) is a monoclonal antibody that binds to the CD150 (SLAM) protein. CD150 is a cell surface receptor expressed on various immune cell types, including T cells, B cells, and dendritic cells. This antibody can be used for the detection and study of CD150-expressing cells.

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Lab products found in correlation

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7 protocols using anti cd150 tc15 12f12.2

Hematopoietic Stem Cell Immunophenotyping

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Cells were analyzed by flow cytometry with FACS Fortessa or LSR II (BD) and FACSDiva software (BD Biosciences) or FlowJo software (Tree Star). The following monoclonal antibodies were used: anti- CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), and anti-Sca-1 (D7) from BD Biosciences; anti-CD150 (TC15- 12F12.2) from Biolegend; anti-CD16/32 (93) and anti-CD127 (A7R34) from eBioscience. In all the FACS plots, indicated are the percentages (%) of the gated fraction.

Nakagawa M.M., Chen H, & Rathinam C.V. (2018). Constitutive Activation of NF-κB Pathway in Hematopoietic Stem Cells Causes Loss of Quiescence and Deregulated Transcription Factor Networks. Frontiers in Cell and Developmental Biology, 6, 143.

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Isolation of Hematopoietic Stem Cells

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HSCs (Tie2⁺CD150⁺CD48^low/−CD135⁻ LKS) were harvested from the Tie2 GFP mouse and sorted as described in Ito, K et al.^{18 (link)}, using monoclonal antibodies specific for the following: CD41 (eBioMWRag30), CD135 (Avas12a1), CD34 (RAM34), c-Kit (2B8), Sca-1 (E13-161.7), CD3e (145-2C11), CD4 (L3T4), CD8 (53-6.72), B220 (RA3-6B2), TER-119 (TER-119), Gr-1 (RB6-8C5), CD11b (M1/70), IgM (II/41), CD19 (eBio1D3), F4/80 (BM8), CD25 (PC61), CD44 (IM7), CD71 (R17217), CD127 (A7R34), CD45.2 or Ly5.2, (104) CD45.1 or Ly5.1 (A20) and NK-1.1 (PK136); all were from eBioscience. Anti-CD150 (TC15-12F12.2) and CD48 (HM48-1) antibodies were from BioLegend. We used a mixture of monoclonal antibodies against CD4, CD8, CD3e, B220, TER-119, CD11b, Gr-1, IgM, CD19, CD127, and NK-1.1 as a lineage marker (Lineage)^{18 (link)}.

Turcotte R., Alt C., Runnels J.M., Ito K., Wu J.W., Zaher W., Mortensen L.J., Silberstein L., Côté D.C., Kung A.L., Ito K, & Lin C.P. (2017). Image-guided transplantation of single cells in the bone marrow of live animals. Scientific Reports, 7, 3875.

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Multiparametric Flow Cytometry Analysis

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Cells were analyzed by flow cytometry with FACSForettsa or LSR II (BD) and FACSDiva software (BD Biosciences) or FlowJo software (Tree Star). The following monoclonal antibodies were used: anti-B220 (RA3–6B2), anti- CD19 (1D3), anti- CD3ε (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti-CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti-Gr-1 (RB6-8C5), anti-Sca-1 (D7), anti-CD71 (C2), anti-CD44 (IM7), anti-CD62L (MEL-14) and anti-Ter119 (TER119); from BD Biosciences; anti-CD25 (PC61) and anti-CD150 (TC15- 12F12.2) from Biolegend; anti-CD16/32 (93), anti-Ly6D (49-H4), anti-CD11C (N418) anti-NK1.1 (PK136)anti-CD41 (MWReg30), anti-CD105 (MJ7/18) and anti-CD127 (A7R34) from eBioscience. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–Peridinin Chlorophyllprotein–Cyanine 5.5 (551419; BD Biosciences) or Streptavidin-Allophycocyanin-Cy7 (554063; BD Biosciences). In all the FACS plots, indicated are the relative frequencies (%) of the gated fraction.

Nakagawa M.M, & Rathinam C.V. (2019). A20 deficiency in hematopoietic stem cells causes lymphopenia and myeloproliferation due to elevated Interferon-γ signals. Scientific Reports, 9, 12658.

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Multiparametric Flow Cytometry Analysis

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Cells were analyzed by flow cytometry with Attune Nxt (Thermofisher) and FlowJo software (Tree Star). The following monoclonal antibodies were used: anti- CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti-Sca-1 (D7), anti-B220 (RA3-6B2), anti- CD19 (1D3), anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti– Gr-1 (RB6-8C5), and anti-Ter119 (TER119; from BD Biosciences); anti-CD150 (TC15-12F12.2) from Biolegend; anti-CD16/32 (93) from eBioscience. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD), streptavidin-allophycocyanin-Cy7 (554063; BD), streptavidin-super bright 650 (Biolegend). In all the FACS plots, indicated are the percentages (%) of the gated fraction.

Lakhan R, & Rathinam C.V. (2021). Deficiency of Rbpj Leads to Defective Stress-Induced Hematopoietic Stem Cell Functions and Hif Mediated Activation of Non-canonical Notch Signaling Pathways. Frontiers in Cell and Developmental Biology, 8, 622190.

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Multiparametric Flow Cytometry Analysis

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Cells were analyzed by flow cytometry with FACS Fortessa, LSR II (BD), Attune NXT and FACSDiva software (BD Biosciences) or FlowJo software (Tree Star). The following monoclonal antibodies were used: anti- CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti-Sca-1 (D7), anti-B220 (RA3-6B2), anti- CD19 (1D3), anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CDllb (M1/70), anti– Gr-1 (RB6-8C5) and anti-Ter119 (TER119; from BD Biosciences); anti-CD150 (TC15-12F12.2) from Biolegend; anti-CD16/32 (93) from eBioscience; anti-Mpl (AMM2) from IBL. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD) or streptavidin-allophycocyanin-Cy7 (554063; BD). In all the FACS plots, indicated are the percentages (%) of the gated fraction.

Nakagawa M.M., Davis H, & Rathinam C.V. (2018). A20 deficiency in multipotent progenitors perturbs quiescence of hematopoietic stem cells. Stem cell research, 33, 199-205.

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Multiparametric Flow Cytometry Analysis

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Nakagawa M.M., Davis H, & Rathinam C.V. (2018). A20 deficiency in multipotent progenitors perturbs quiescence of hematopoietic stem cells. Stem cell research, 33, 199-205.

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Multiparametric flow cytometry analysis

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Cells were analyzed by flow cytometry with either LSRFortessa or LSRII (both BD) and FACSDiva software (BD) or FlowJo software (Tree Star). Cell sorting was performed with a FACSAria (BD). The following monoclonal antibodies were used: anti-B220 (RA3-6B2), anti-CD19 (1D3), anti-CD3ε (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti-CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti–Gr-1 (RB6-8C5), anti–Sca-1 (D7), and anti-Ter119 (TER119; from BD); anti-CD150 (TC15-12F12.2; from BioLegend); and anti-CD16/32 (93) and anti-CD127 (A7R34; from eBioscience). Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD) or streptavidin-allophycocyanin-Cy7 (554063; BD). In all of the FACS plots, the relative frequencies (%) of the gated fraction are indicated.

Nakagawa M.M., Thummar K., Mandelbaum J., Pasqualucci L, & Rathinam C.V. (2015). Lack of the ubiquitin-editing enzyme A20 results in loss of hematopoietic stem cell quiescence. The Journal of Experimental Medicine, 212(2), 203-216.

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