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Gf uct240

Manufactured by Olympus
Sourced in Japan

The GF-UCT240 is a high-performance, versatile upright compound microscope designed for a wide range of laboratory applications. It features a UIS2 (Universal Infinity-Corrected) optical system, providing superior image quality and clarity. The microscope is equipped with a quadruple nosepiece, allowing for the use of multiple objectives for different magnification needs. It also includes a stage with a coaxial coarse and fine focusing mechanism for precise and smooth adjustments. The GF-UCT240 is a reliable and efficient tool for various laboratory tasks that require high-quality microscopic observation and analysis.

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25 protocols using gf uct240

1

Distinguishing Mucinous and Serous Cystic Neoplasms

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All procedures were performed between December 2010 and December 2020 at the endoscopic center of Inha University Hospital and Asan Medical Center. An ultrasound scanning system (SSD 5500, 5 and 10; Aloka, Tokyo, Japan) with linear (GF-UCT 240; Olympus Optical corp. Ltd., Tokyo, Japan) and radial (GF-UE 260; Olympus Optical corp. Ltd., Tokyo, Japan) echoendoscopes was used. The examination was conducted by endosonographers with at least five years of experience. Endosonographers at two separate sites independently acquired and completed the analysis of the EUS images. The clinical data were analyzed for image findings and pathological diagnosis of the patients. To validate the diagnosis of unclear cases, FNA or histology analysis through surgery was performed and 44% of the patients experienced this additional level of diagnosis. This study was reviewed and approved by the Institutional Review Boards of Inha University Hospital (2020-05-002) and of Asan Medical Center (2020-1290-0001).
The EUS data were acquired from 47 MCN and 31 SCN patients at the 1st hospital and 13 MCN and 18 SCN patients at the 2nd hospital. The characteristics of the patients and lesions for this study are summarized in Table 1. Figure 1 shows a sample of histology images for MCN (Figure 1A) and SCN (Figure 1B) cases.
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2

Endoscopic Ultrasound and ERCP Procedures

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All EUS procedures were performed with a curved linear-array echoendoscope (Olympus GF-UE260, GF-UCT240; Olympus Optical Co., Tokyo, Japan) with a universal ultrasound processor (EU-ME2; Olympus Optical Co., Tokyo, Japan). All ERCP procedures were performed with a therapeutic duodenoscope (JF240, JF260V, TJF 260V; Olympus Optical Co., Tokyo, Japan). A single-lumen cannula (ERCP catheter; MTW Endoskopie, Wesel, Germany) and a guidewire (length, 450 cm; diameter, 0.06 cm; VisiGlide2; Olympus Medical Systems, Tokyo, Japan) were used for cannulation during ERCP. Three endoscopists, all of whom had experience of performing more than 300 CLAEUS procedures and more than 700 ERCP procedures, performed or supervised the CLAEUS and ERCP.
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3

EUS-FNB Technique for Lesion Diagnosis

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EUS-FNB was performed by an expert endosonographer with experience of more than 100 EUS-FNB procedures using a linear-array echoendoscope (GF-UCT 240 or GF-UCT 260, Olympus Optical, Tokyo, Japan; or EG-530UT2, FUJIFILM, Tokyo, Japan) with the patient under conscious sedation. All patients underwent EUS-FNB using a 20G needle with a core trap (EchoTip ProCore, Cook Medical, Bloomington, IN, USA). The slow-pull technique was used while fanning the needle throughout the lesion twenty times after the mass had been punctured.[5 (link)] Tissue samples were expelled into formalin bottles with a stylet or air and then processed for histological evaluation; no rapid on-site evaluation was performed. The sample was not divided for cytologic examination including cell blocks. Samples obtained from additional needle passes were used to ensure an accurate diagnosis. The number of additional passes was determined by the physician.
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4

EUS-FNB for Pancreatic Disease Diagnosis

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EUS-FNB was performed by one of four expert endoscopists with experience of >1000 cases for pancreatic diseases. Patients underwent conventional EUS-FNB using a linear-array echoendoscope (GF-UCT 240, GF-UCT 260; Olympus Optical Co., Tokyo, Japan) and a 19- or 22-gauge needle (EZ Shot 3; Olympus Medical, Tokyo, Japan, Acquire; Boston Scientific, MA, USA). For histological diagnosis, tissue was obtained through at least two needle passes, and if there was no visible core tissue, up to two more passes were performed. Afterwards, samples for biobanking were obtained through 1–2 needle passes to obtain visible core tissue. These specimens for biobanking were placed in a cryotube and then immediately frozen in liquid nitrogen and stored in a deep freezer at −80 °C until DNA extraction. The HiGene Genomic DNA Prep Kit (GD141-100, BIOFACT, Daejeon, Korea) was used for DNA extraction.
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5

Endoscopic Ultrasound-Guided Fine Needle Aspiration

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EUS was performed using a convex linear-array endoscope (GF-UCT260 or GF-UCT240; Olympus Medical Systems, Tokyo, Japan; EG-580UT; Fujifilm Corp., Tokyo, Japan) connected to a US device (EU-ME1 or EU-ME2 PREMIER PLUS; Olympus; SU-1 ultrasound processor; Fujifilm Corp.). A 22- and/or 25-gauge needle (EZ-Shot 3; Olympus Medical Systems Group, Tokyo, Japan; Acquire; Boston Scientific Corporation, Marlborough, MA, USA; Expect; Boston Scientific Corporation) was used for EUS-FNA. The white tissue parts obtained from the samples were used for histological evaluation, whereas the other parts were used for cytological evaluation. One cytology slide was subjected to modified Giemsa staining for rapid on-site evaluation and confirmation of the adequacy of the obtained sample.
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6

EUS-Guided Tissue Sampling Procedure

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All procedures were performed according to the standardized protocols with left-lateral decubitus position and under moderate conscious sedation using meperidine, propofol, and/or midazolam. EUS was performed by a single experienced endosonographer (C.C.) who performs over 300 interventional cases per year. Following the identification of a target lesion using a radial scanning echoendoscope (GF-UE260; Olympus Medical Systems, Tokyo, Japan), EUS-guided tissue sampling was performed with a linear echoendoscope (GF-UCT240; Olympus Medical Systems).
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7

EUS-FNA for Pancreatic Mass Sampling

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EUS-FNA specimen was acquired by two experienced endoscopists (Professors: J.K.R. and Y.-T.K.) under the conscious sedation using meperidine and midazolam in pancreatobiliary clinic of GI division at Seoul National University Hospital. After the sedation, pancreatic mass was identified and explored the optimal site for EUS-FNA. The procedure was carried out using a linear array echo endoscope (GF-UM-2000 or GF-UCT 240; Olympus, Tokyo, Japan) with the 22G FNA needle (EchoTip Ultra; Wilson Cook Medical Inc.). EUS-FNA was performed as follows; after proper targeting to a mass, the FNA needle was passed through the biopsy channel and advanced into the target lesion under EUS guidance. To-and-fro movements were then performed 10 to 20 times with suction using a 10-mL syringe. After retracting the needle, the aspirated contents were expressed onto glass slides with the stylet smeared, and fixed in 99% ethanol. Pancreas mass located head or uncinate process was acquired by transduodenal passes while the body or tail mass was acquired through the puncturing stomach wall. The endoscopists assessed the gross adequacy of the sample, and selected the number of needle passes due to the absence of on-site cytopathologist. Additional needle passes to obtain study samples were not needed.
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8

Endoscopic Ultrasound-Guided Fine-Needle Aspiration

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We performed EUS using a convex linear-array endoscope (GF-UCT260 or GF-UCT240; Olympus Medical Systems, Tokyo, Japan and EG-580UT: Fujifilm Corp, Tokyo, Japan) connected to an ultrasound device (Prosound SSD-α10; Hitachi Aloka, Tokyo, Japan, EU-ME1 or EU-ME2 PREMIER PLUS; Olympus, SU-1 ultrasound processor; Fujifilm Corp). EUS-FNA was performed with a 22- and/or 25-gauge needle (EZ-Shot 3; Olympus Medical Systems; Acquire, Boston Scientific Corp., Marlborough, MA, USA; EchoTip ProCore, Cook Medical, Bloomington, IN, USA; SonoTip II, Medi-Globe GmbH, Rohrdorf, Germany; Expect, Boston Scientific Corp.).
EUS-FNA was repeated until sufficient sampling had been performed for rapid on-site evaluation or until the endoscopist believed that further sampling was unlikely to increase the amount of tissue.
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9

Endoscopic Ultrasound-Guided Biliary Drainage Techniques

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The detailed methods of EUS-BD have been previously described (23) (24) (25) . For EUS-CDS, fully covered WallFlex Biliary RX Stents (Boston Scientific Japan), with lengths of 4 and 6 cm, respectively, and expanded diameters of 10 mm, were mainly used. For EUS-HGS, fully covered Niti-S biliary stents (Taewoong Medical, Seoul, Korea), with lengths of 10 and 12 cm and fully expanded diameters of 6 mm, were used. In addition, linear echoendoscopes (GF-UCT240 or TGF-UC260J; Olympus), 19-gauge needles for fine-needle aspiration (Sono Tip Pro control Ⓡ ; Medi-Globe GmbH, Rosenheim, Germany or Expect ; Boston Scientific Japan), a needle knife (Zimmon papillotomy knife; Cook Endoscopy, Bloomington, USA), an electrical dilator (Cysto-Gastro-Set; Century Medical, Tokyo, Japan), and biliary guidewires (VisiGlide ; Olympus) were also used during these procedures.
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10

Radial Scanning Echoendoscopy and EUS-FNA

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Radial scanning echoendoscopes (GF-UM20, GF-UM240, GF-UM2000, UM-DP20, and UM-DP12; Olympus Co., Tokyo, Japan) were used to perform EUS. EUS-FNA was performed with the use of convex array echoendoscopes (GF-UCT260, GF-UCT240, XGF-UCT160, GF-UC2000P; Olympus Co., Ltd.). The following puncture needles were used: 19-gauge needles (Wilson-Cook, Winston Salem, NC, United States), 22-gauge needles (NA-200H, Olympus Co., Ltd.), and 25-gauge needles (Echochip, Wilson-Cook). The aspiration pressure was 10 to 20 cc, and “in-and-out motion” was continued for 20 strokes (occasionally, 10 strokes). Puncture was performed 2 to 6 time (median, 3 times).
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