Heat inactivated human ab serum

Manufactured by Thermo Fisher Scientific

Heat-inactivated human AB serum is a cell culture media supplement derived from human blood. It is processed through heat inactivation to inactivate any potential pathogens.

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Lab products found in correlation

Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) G csf, by BioLegend (1 mentions) Recombinant human il 4, by R&D Systems (1 mentions) Anti cd14 antibody, by BD (1 mentions) Human m csf, by BioLegend (1 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) X vivo 15 medium, by Lonza (1 mentions) U bottom plate, by Greiner (1 mentions) Recombinant human il 2, by Thermo Fisher Scientific (1 mentions) Facsaria 3, by BD (1 mentions)

Close spelling variants of this product

Heat-inactivated human serum Heat-inactivated Human AB serum AB Serum Human serum

4 protocols using heat inactivated human ab serum

Monocyte Differentiation into Macrophages

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Peripheral blood mononuclear cells were isolated from buffy coats from healthy donors (Blutspende Zürich, Zurich, Switzerland) by density gradient centrifugation using Ficoll-paque PLUS (Cat: 17–1440–02; GE Healthcare). The monocyte population was magnetically enriched by negative selection using the Human Monocyte Isolation Kit II (Cat: 130–091–153; MACS, Miltenyi Biotech). The monocyte purity was >95% as confirmed by FACS analysis with an anti-CD14 antibody (BD Biosciences).
Freshly isolated human monocytes were differentiated into macrophages by incubation with either 50 ng/mL of human M-CSF (Cat: 14–8789) or G-CSF (Cat: 578602) (both from BioLegend) in RPMI medium (Cat: 61870–010; Gibco) supplemented with 1% heat-inactivated human AB serum (Cat: 34005100; Invitrogen), for up to 8 d. 20 ng/mL of recombinant human IL-4 (Cat: 204-IL; R&D Systems) was used as a control to induce M2-type macrophage differentiation and anti-human G-CSF (1 μg/mL, clone BVD13–3A5, Cat: 502101; BioLegend) or isotype control (rat IgG1 Cat: 400401) was used to neutralize G-CSF in MDA-MB-231 conditioned medium.

Hollmén M., Karaman S., Schwager S., Lisibach A., Christiansen A.J., Maksimow M., Varga Z., Jalkanen S, & Detmar M. (2015). G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology, 5(3), e1115177.

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Human CD4+ T Cell Culture

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Freshly isolated human CD4⁺ T cells were cultured in Vivo15 Medium supplemented with 2 mM glutamine, penicillin (50 U/ml) (Sigma Aldrich), streptomycin (50 μg/ml) (Sigma Aldrich), 100 U/ml human recombinant IL-2 (ReproTech) and 5% (vol/vol) heat-inactivated human AB serum (Invitrogen) in 96-well U bottom plates (Bio-Greiner one). in an humidified CO₂ incubator.

Kälin S., Becker M., Ott V.B., Serr I., Hosp F., Mollah M.M., Keipert S., Lamp D., Rohner-Jeanrenaud F., Flynn V.K., Scherm M.G., Nascimento L.F., Gerlach K., Popp V., Dietzen S., Bopp T., Krishnamurthy P., Kaplan M.H., Serrano M., Woods S.C., Tripal P., Palmisano R., Jastroch M., Blüher M., Wolfrum C., Weigmann B., Ziegler A.G., Mann M., Tschöp M.H, & Daniel C. (2017). A Stat6/Pten axis links cold exposure with T cell tolerance in adipose tissue. Cell metabolism, 26(3), 475-492.e7.

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Culturing Human CD4+ T Cells

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Freshly isolated human CD4⁺ T cells were cultured in X-Vivo15 Medium (Lonza) supplemented with 2 mM glutamine, 100 U/ml human recombinant IL-2 (ReproTech), 50 U/ml penicillin, 50 mg/ml streptomycin (Sigma Aldrich), and 5% heat-inactivated human AB serum (Invitrogen) at 37 °C in a humidified CO₂ incubator. Cell culture-treated 96-well U bottom plates were used (Bio-Greiner one).

Scherm M.G., Serr I., Zahm A.M., Schug J., Bellusci S., Manfredini R., Salb V.K., Gerlach K., Weigmann B., Ziegler A.G., Kaestner K.H, & Daniel C. (2019). miRNA142-3p targets Tet2 and impairs Treg differentiation and stability in models of type 1 diabetes. Nature Communications, 10, 5697.

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Activation and Expansion of Human Naive CD8+ T Cells

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96 well U-bottom plates (Greiner bio-one) were pre-coated with 5 μg/ml anti-CD3e (UCHT1, BioLegend) and 15 μg/ml anti-CD28 (CD28.2, BioLegend) in 0.1 M sodium bicarbonate buffer pH = 8.2 for 1 h at 37 °C, followed by 4 °C. Cells were sorted with a FACSAriaIII (BD) cell sorter for purity. Human naïve live CD8a⁻CD11b⁻CD14⁻CD19^-CD3⁺CD4⁺CD45RA⁺CD45RO⁻CD127⁺CD25^- T cells were cultured in x-Vivo15 Medium supplemented with 2 mM glutamine, penicillin (50 U/ml), streptomycin (50 μg/ml), and 5% (vol/vol) heat-inactivated human AB serum (Invitrogen) in the presence of 100 U/ml human recombinant IL-2 (Peprotech) in an humidified incubator at 37 °C with 5% CO₂. TCR stimulation was limited to 18 h by transferring cells into uncoated wells. Cells were cultured for additional 36 h without further TCR stimulation.

Becker M., Serr I., Salb V.K., Ott V.B., Mengel L., Blüher M., Weigmann B., Hauner H., Tschöp M.H, & Daniel C. (2019). Short-term cold exposure supports human Treg induction in vivo. Molecular Metabolism, 28, 73-82.

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