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3 protocols using tamsulosin

1

Pharmacological Agents for Urological Studies

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Krebs solution was obtained from Sigma Aldrich (St. Louis, MO, USA). Silodosin (Recordati, Milan, Italy), tamsulosin (Cayman, Ann Arbor, MI, USA), and naftopidil (Cayman) were procured and used. Silodosin and tamsulosin were dissolved in ethanol and naftopidil in methanol (Merck, Kenilworth, NJ, USA) and stored as stock solutions: Silodosin (1 mM), tamsulosin (400 nM), and naftopidil (200 µM) [15 (link)16 17 ].
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2

HuH-7 Cell Compound Screening

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Small-scale HuH-7R-targeted quinazoline-derived small molecular compound screening was performed via IC50 ranking based on MTT assays. HuH-7R cells were seeded into 96-well plates and exposed to concentration gradients of prazosin (Sigma-Aldrich), cyproheptadine (Sigma-Aldrich), phentolamine (Sigma-Aldrich), doxazosin (Sigma-Aldrich), terazosin (Sigma-Aldrich), WB-4101 (Sigma-Aldrich), tamsulosin (Sigma-Aldrich), clozapine (Sigma-Aldrich), bunazosin (Angene Chemical), and ketanserin (Sigma-Aldrich) for 72 h. The IC50 value of each compound was determined and ranked.
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3

Pharmacological Interventions Protocol

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Quercetin, phenylephrine, tamsulosin, and cyclodextrin were purchased from Sigma‐Aldrich. The specific use of these drugs is specified in detail in each protocol above. Buprenorphine hydrochloride (Buprecare) and isoflurane (IsoFlo) were obtained from AST Farma, Oudewater, The Netherlands. Carprofen (Norocarp) was obtained from Norbrook Laboratories, Newry, UK.
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