Pyrrole (reagent grade,
98%, Sigma-Aldrich) solution (0.1 M) was prepared in 0.1 M NaCl supporting
electrolyte. The antibody (Ab) of interest (seeTable S3 ) was diluted in Pyrrole solution to a final concentration
of 6 μg/mL. Electropolymerization of Pyrrole to polyPyrrole
(PPy) was performed via cyclic voltammetry between 0.0 and +0.95 V
with respect to the Ag/AgCl reference electrode at the scan rate of
50 mV/s. After polymerization, electrodes were washed with phosphate-buffered
saline (PBS) buffer and kept in PBS until use. The surface topography
of electrodes was measured using an atomic force microscope (NX-10,
Park Systems) equipped with CONTSCR cantilevers (NanoWorld). Topography
images were generated using XEI software (Park Systems).
98%, Sigma-Aldrich) solution (0.1 M) was prepared in 0.1 M NaCl supporting
electrolyte. The antibody (Ab) of interest (see
of 6 μg/mL. Electropolymerization of Pyrrole to polyPyrrole
(PPy) was performed via cyclic voltammetry between 0.0 and +0.95 V
with respect to the Ag/AgCl reference electrode at the scan rate of
50 mV/s. After polymerization, electrodes were washed with phosphate-buffered
saline (PBS) buffer and kept in PBS until use. The surface topography
of electrodes was measured using an atomic force microscope (NX-10,
Park Systems) equipped with CONTSCR cantilevers (NanoWorld). Topography
images were generated using XEI software (Park Systems).