Mycoplasma elimination reagent

THP1, Jurkat, Hela, L929 and RAW264.7 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). THP1-Lucia ISG cells were purchased from Invivogen. cGAS^-/- Hela cells were a kind gift from Dr. Zhengfan Jiang (School of Life Science, Peking University, Beijing, China). Human peripheral blood mononuclear cells (PBMCs) were purchased from LDEBIO (Guangzhou, China). THP1, THP1-Lucia ISG cells, Jurkat and PBMCs were cultured in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gbico) and 0.1% Normocin (InvivoGen, Cat# ant-nr) and 0.1% mycoplasma elimination reagent (Yeasen, Cat# 40607ES03) at 37 °C with 5% CO₂. Hela, cGAS^-/- Hela, L929 and RAW264.7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gbico) supplemented with 10% FBS and 0.1% Normocin (InvivoGen, Cat# ant-nr) and 0.1% mycoplasma elimination reagent (Yeasen, Cat# 40607ES03) at 37 °C with 5% CO₂.