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Pgd2 mox eia kit

Manufactured by Cayman Chemical
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The PGD2-MOX EIA kit is a competitive enzyme immunoassay designed for the quantitative measurement of 11β-Prostaglandin F2α (PGD2-MOX) in biological samples. This kit utilizes a monoclonal antibody to PGD2-MOX and an acetylcholinesterase:PGD2-MOX (Tracer) conjugate.

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Lab products found in correlation

Oasis hlb column, by Waters Corporation (2 mentions)

3 protocols using pgd2 mox eia kit

1

Measuring PGD2 Production in WAT

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The PGs in WAT were extracted as described previously29 (link). Production of PGD2 was measured by using a PGD2 MOX EIA Kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s instructions.
Fujimori K., Aritake K., Oishi Y., Nagata N., Maehara T., Lazarus M, & Urade Y. (2019). L-PGDS-produced PGD2 in premature, but not in mature, adipocytes increases obesity and insulin resistance. Scientific Reports, 9, 1931.
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2

BAL Fluid Eicosanoid Extraction

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0.5 ml of BAL fluid was mixed with 0.5 ml of ice-cold acetone, incubated on ice for 5 minutes and centrifuged for 10 minutes at 3000g at 4°C. After supernatant aspiration, the pellet was extracted with 1 ml of ice-cold acetone and centrifuged again. The acetone extracts were combined and the acetone evaporated under nitrogen. All Samples were then methoximated (PGD2-MOX EIA kit (Cayman Chemical), and purified on Oasis HLB columns (Waters Corporation) equilibrated with methanol/0.2% formic acid. Methanol eluants were evaporated in a Savant Speed Vac concentrator and samples dissolved in Cayman EIA buffer before EIA analysis, according to manufacturer’s instructions.
Kelvin A.A., Degousee N., Banner D., Stefanski E., Leόn A.J., Angoulvant D., Paquette S.G., Huang S.S., Danesh A., Robbins C.S., Noyan H., Husain M., Lambeau G., Gelb M., Kelvin D.J, & Rubin B.B. (2014). Lack of group X secreted phospholipase A2 increases survival following pandemic H1N1 influenza infection. Virology, 454, 78-92.
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3

Quantification of Prostaglandin D2 in BAL Fluid

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0.5 ml of BAL fluid was mixed with 0.5 ml of ice-cold acetone, incubated on ice for 5 min and centrifuged for 10 min at 3000g at 4 °C. After supernatant aspiration, the pellet was extracted with 1 ml of ice-cold acetone and centrifuged again. The acetone extracts were combined and the acetone evaporated under nitrogen. All the samples were then methoximated (PGD2-MOX EIA kit (Cayman Chemical), and purified on Oasis HLB columns (Waters Corporation) equilibrated with methanol/0.2% formic acid. Methanol eluants were evaporated in a Savant Speed Vac concentrator and samples dissolved in Cayman EIA buffer before EIA analysis, according to the manufacturer׳s instructions.
Kelvin A.A., Degousee N., Banner D., Stefanski E., Leόn A.J., Angoulvant D., Paquette S.G., Huang S.S., Danesh A., Robbins C.S., Noyan H., Husain M., Lambeau G., Gelb M., Kelvin D.J, & Rubin B.B. (2014). Lack of group X secreted phospholipase A2 increases survival following pandemic H1N1 influenza infection. Virology, 454, 78-92.
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