Anti mouse il 2

Manufactured by Thermo Fisher Scientific

Anti-mouse IL-2 is a laboratory reagent that can be used to detect and quantify the presence of interleukin-2 (IL-2) in mouse samples. It is a specific antibody that binds to mouse IL-2 and allows for its measurement using various immunoassay techniques.

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Mouse IL-2 (32 citations) Anti-IL-2 (14 citations) Anti-mouse-IL-2 antibody (2 citations)

3 protocols using anti mouse il 2

Detailed Immunological Antibody Protocols

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The antibodies used in this study for flow cytometry include: anti-mouse CD8 (#563786), anti-mouse CD25 (#558642), anti-mouse TCRβ (#553174), anti-human CD3 (#563798), anti-human CD8 (#563795), and anti-human TCRαβ (#563826) from BD Biosciences; anti-mouse CD4 (#100544), anti-mouse IFN-γ (#505835), anti-human LFA-1 (#363404), anti-human CD11a (#301208), anti-human CD18 (#302114), anti-human CD25 (#302625), and anti-human CD69 (#310920) from BioLegend; anti-mouse CD4 (#17-0042-83), anti-mouse CD4 (#17-0041-83), anti-mouse CD69 (#25-0691-82), anti-mouse TNF (#17-7321-82), anti-mouse IL-2 (#12-7021-82), anti-human CD4 (#17-0048-2), and anti-human CD8 (#11-0088-41) from eBioscience.
The antibodies used for immunoprecipitation and immunoblotting include: anti-Cdc7 (#ab10535), anti-Dbf4 (#ab124707), anti-RNA polymerase II (#ab817) and anti-RNA polymerase II (phospho S2, # ab193468) from Abcam; anti-PLCγ (#610027) and anti-ERK (#610031) from BD Biosciences; anti-LAT (#641102) from BioLegend; anti-GAPDH (#2118), anti-pPLCγ (#2821), anti-ZAP70 (#2709), anti-pZAP70 (#2717), anti-pERK (#4370), anti-pLAT (#3584), anti-pLck (#6943), and anti-NF-κB (#3035) from Cell Signaling; anti pMCM2 S40 (#GTX62847) from GeneTex; anti-MCM2 (#MA5-15895) from Pierce; and anti-Lck (#sc-433) from Santa Cruz.

Chen E.W., Tay N.Q., Brzostek J., Gascoigne N.R, & Rybakin V. (2019). A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Frontiers in Immunology, 10, 1718.

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Chikungunya Virus Infection Model

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Three-week-old WT or CD4 KO C57/BL6J female mice were inoculated subcutaneously in the ventral side of the right hind footpad with 10⁶ pfu CHIKV diluted in 30 μl PBS. Following CHIKV infection, PBS, 1.5 μg murine IL-2 (Peprotech), 50 μg of rat anti-mouse IL-2 (JES6-1) monoclonal antibody (eBioscience) or 1.5 μg murine IL-2 in complex with 50 μg anti-mouse IL-2 mAb were injected i.p daily on 3, 4 and 5 dpi. IL-2 Ab Cx pre-treatment was carried out as described21 (link). Joint footpad swelling was determined daily by the measurement of the height and the breadth of the footpad using a caliper and quantified as (height x breadth). The degree of inflammation was expressed as relative increase compared to pre-infection (d 0) with the following formula: [(x –d 0)/d 0] where x is footpad size measurements for respective dpi4 (link)25 (link)27 (link)66 (link).

Lee W.W., Teo T.H., Lum F.M., Andiappan A.K., Amrun S.N., Rénia L., Rötzschke O, & Ng L.F. (2016). Virus infection drives IL-2 antibody complexes into pro-inflammatory agonists in mice. Scientific Reports, 6, 37603.

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Treg Expansion Assay in Mice

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PBS, 1.5 μg murine IL-2 (Peprotech), 50 μg anti-mouse IL-2 (JES6-1) (eBioscience), or 1.5 μg murine IL-2 complexed with 50 μg anti-mouse IL-2 were injected intrperitoneally (i.p.) daily for 3 days. Fifteen microliters of blood were collected from the tail vein to check for Treg expansion. Briefly, blood was lysed and fixed using BD fluorescence-activated cell sorting (FACS) lysing buffer (BD Biosciences) and washed twice with PBS. Cells were stained with anti-CD4 (BD Biosciences) and anti-CD25 (eBioscience). Intracellular staining of Foxp3 was done using a Foxp3 staining buffer set (eBioscience) and anti-Foxp3 following the manufacturer's instructions. Data were acquired using a BD LSRFortessa cell analyzer. Dead cells and duplets were excluded in all analyses using forward and side scatter gating. Results were analyzed with FlowJo version X software (Tree Star, Inc.).

Lee W.W., Teo T.H., Her Z., Lum F.M., Kam Y.W., Haase D., Rénia L., Rötzschke O, & Ng L.F. (2015). Expanding Regulatory T Cells Alleviates Chikungunya Virus-Induced Pathology in Mice. Journal of Virology, 89(15), 7893-7904.

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