Clinical glioma samples (WHO I-IV) were histopathologically and clinically diagnosed at the Daping hospital of Third Military Medical University. Non-tumoral brain specimens were taken from a standard distance from the margin of resected neoplastic tissues of patients with glioma who underwent surgery. IHC procedure to detect URGCP (Antibody: Sigma (human) and Shangon biotech (mice)) and Ki67 were performed using CECTASTAIN ABC Detection system (VECTOR) according to the manufacturer’s instruction. The results of URGCP and Ki67 detection were determined according to the percentage of positive cell number to total number in 10 randomly selected microscopic fields.
Cectastain abc detection system
Manufactured by Vector Laboratories
The CECTASTAIN ABC Detection system is a reagent kit used for the detection of antigens in tissue sections and cell preparations. It utilizes an avidin-biotin-peroxidase complex (ABC) to amplify the signal, resulting in enhanced sensitivity. The kit includes the necessary components for the immunohistochemical detection of target antigens.
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Lab products found in correlation
2 protocols using cectastain abc detection system
1
Quantifying URGCP and Ki67 in Glioma
2
SOCS6 Immunohistochemistry Protocol
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The IHC staining of SOCS6 was performed using CECTASTAIN ABC Detection system (VECTOR). The result was determined according to the percentage of positive cell number to total cell number in 10 randomly selected microscopic fields.
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