Angiotensin I human acetate salt hydrate, angiotensin II, and formic acid were purchased from Sigma-Aldrich (St. Louis, MO). Coated fused silica PicoTip emitters (360 μm OD, 75 μm ID, 30 μm tip ID) were purchased from New Objective (Waltham, MA). Unless otherwise specified, all solvents used were of HPLC grade and purchased from Honeywell-Burdick & Jackson (Muskegon, MI). Low-purity nitrogen was used as the gas-flow source.
Angiotensin 1 human acetate salt hydrate
Manufactured by Merck Group
Sourced in United States
Angiotensin I human acetate salt hydrate is a laboratory reagent used in research applications. It is a synthetic form of the peptide hormone angiotensin I, which plays a role in the regulation of blood pressure. The product is provided as a salt hydrate for use in various analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Angiotensin 2, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Neurotensin, by Merck Group (1 mentions)
Substance p acetate salt hydrate, by Merck Group (1 mentions)
Melittin, by Merck Group (1 mentions)
Substance p, by Merck Group (1 mentions)
Ubiquitin, by Merck Group (1 mentions)
Cesium triiodide, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions)
4 protocols using angiotensin 1 human acetate salt hydrate
1
Mass Spectrometry Peptide Analysis
2
Peptide Solutions for Mass Spectrometry
3
Synthesis and Characterization of ASA–buten–PtCl3
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Angiotensin I (human, acetate salt hydrate, > 90%), ubiquitin (from bovine erythrocytes, > 98%), and substance P (acetate salt hydrate, > 95%) were obtained from Sigma-Aldrich and used as received. Solvents were purchased in MS quality from Sigma-Aldrich. Synthesis, stability and biological data of ASA–buten–PtCl3 are reported elsewhere [20 (link)].
4
Mass Spectrometry Analyte Preparation
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Cesium triiodide (CsI3, 99.9%), angiotensin I human acetate salt hydrate (90%), and α-cyano-4-hydroxycinnamic
acid (CHCA) were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.).
Organic solvents used in sample preparation, including acetonitrile
(99.9%) and ethanol (99.9%), were sourced from J. T. Baker Avantor
(Radnor, PA, U.S.A.). Distilled deionized water used in the experiment
(with a resistivity of 18.2 MΩ-cm) was produced by the Merck
milli-Q purification system (Darmstadt, Germany).
The CsI3 analyte solution was prepared using 50% ethanol with a concentration
of 0.1 mmol/mL. One microliter of the analyte solution was directly
deposited onto the sample plate and dried in a vacuum chamber before
analysis. The preparation process did not involve any matrix mixing,
thereby avoiding interference of mass spectra from matrix signals.
The angiotensin I analyte solution was dissolved in distilled deionized
water at a concentration of 10 nmol/mL. The CHCA solution was prepared
using 50% aqueous acetonitrile solution, with a concentration of 0.1
mmol/mL. The CHCA matrix and angiotensin I solutions were premixed
in a final matrix-to-analyte molar ratio of 100:1. Finally, one microliter
of the premixed solution was deposited onto the sample plate and dried
in the vacuum chamber.
acid (CHCA) were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.).
Organic solvents used in sample preparation, including acetonitrile
(99.9%) and ethanol (99.9%), were sourced from J. T. Baker Avantor
(Radnor, PA, U.S.A.). Distilled deionized water used in the experiment
(with a resistivity of 18.2 MΩ-cm) was produced by the Merck
milli-Q purification system (Darmstadt, Germany).
The CsI3 analyte solution was prepared using 50% ethanol with a concentration
of 0.1 mmol/mL. One microliter of the analyte solution was directly
deposited onto the sample plate and dried in a vacuum chamber before
analysis. The preparation process did not involve any matrix mixing,
thereby avoiding interference of mass spectra from matrix signals.
The angiotensin I analyte solution was dissolved in distilled deionized
water at a concentration of 10 nmol/mL. The CHCA solution was prepared
using 50% aqueous acetonitrile solution, with a concentration of 0.1
mmol/mL. The CHCA matrix and angiotensin I solutions were premixed
in a final matrix-to-analyte molar ratio of 100:1. Finally, one microliter
of the premixed solution was deposited onto the sample plate and dried
in the vacuum chamber.
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