Western blots were performed using standard procedures and the following reagents: RHAMM (GTX62573, GeneTex, Irvine), p53 (OP43, Calbiochem, Billerica), p38 (#9212, Cell Signaling Technologies, Danvers), pp38 (#9211, Cell Signaling Technologies, Danvers), β-tubulin (T7816, Sigma-Aldrich Chemie, München) and β-actin (A5316, Sigma-Aldrich Chemie, München). Binding protein for affinitycytochemistry of hyaluronan was biotinylated HABP (Calbiochem, Billerica) and as secondary antibody streptavidin-FITC (Dako, Glostrup) was used. Secondary antibodies for western blotting were IRDye® 800CW goat anti-rabbit IgG, IRDye® 800CW goat anti-mouse IgM, IRDye® 680RD goat anti-rabbit IgG and IRDye® 680LT goat anti-mouse IgM (LI-COR Biotechnology, Bad Homburg). Immunofluorescence secondary antibodies were AF488 goat anti-rabbit IgG (Life Technologies, Carsbad) and AF568 (Fab')2 fragment of goat anti-mouse IgG (H+L) (Life Technologies, Carsbad).
Rhamm
Manufactured by GeneTex
RHAMM is a lab equipment product that functions as a receptor for hyaluronan, a major component of the extracellular matrix. It is involved in cellular processes such as cell migration and signaling.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin fitc, by Agilent Technologies (1 mentions)
Irdye 800cw goat anti mouse igm, by LI COR (1 mentions)
Irdye 680lt goat anti mouse igm, by LI COR (1 mentions)
Goat anti rabbit igg af488, by Thermo Fisher Scientific (1 mentions)
Irdye 680rd goat anti rabbit igg, by LI COR (1 mentions)
β tubulin, by Merck Group (1 mentions)
Irdye 800cw goat anti rabbit igg, by LI COR (1 mentions)
β actin, by Merck Group (1 mentions)
E cadherin, by Proteintech (1 mentions)
E cadherin, by Thermo Fisher Scientific (1 mentions)
2 protocols using rhamm
1
Western blot and immunofluorescence analysis
2
Multimarker Immunohistochemistry of Bladder
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunostaining study was performed according to a previously published method. 37, 38 The bladder sections were double stained with the primary antibody to claudin-4 (mouse monoclonal IgG1; dilution 1:100; Invitrogen, Camarillo, CA), E-cadherin (rabbit polyclonal IgG; dilution 1:100; Proteintech, Chicago, IL), CD44 (mouse monoclonal IgG1; dilution 1:100; Cell Signaling, Danvers, MA), TLR-4 (mouse monoclonal IgG2b; dilution 1:1000; Abcam, Cambridge, MA), RHAMM (rabbit polyclonal IgG; dilution 1:1000; GeneTex, Irvine, CA), cytokeratin (CK)14 (rabbit polyclonal IgG; dilution 1:250; GeneTex), and CK17 (mouse monoclonal IgG2b; dilution 1:10; Novus, Littleton, CO) at 4 C overnight and then incubated with secondary antibody (dilution 1:800; Invitrogen) conjugated to fluorescein isothiocyanate for claudin-4 and CD44 and conjugated to rodamine for E-cadherin and CK14. In each experiment, negative controls without the primary antibody were performed to elucidate nonspecific immunostaining.
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