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4 protocols using l arginine

1

Biogenic TiO2 Nanoparticle Synthesis

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All agents were analytical grade and used without further purification. L-Arginine, L-Cysteine, and Tyzor LA-Lactid acid chelated titanate (Ti-BALDH) were purchased from Macklin (Shanghai, China). Phenol and other chemicals were obtained from Macklin (Shanghai, China). Using Ti-BALDH as raw material, bio-TiO2 NPs were prepared by the bionic dehydrating method [31 (link)]. Briefly, 0.0053 g L-Arginine powder and 0.01 g L-Cysteine were dissolved in 20 mL deionized water and adjusted to pH 7 with concentrated nitric acid. After stirring for 15 min, 200 μL Ti-BALDH (0.294 wt%) was dropped into the mixture and stirred for another 20 min, forming slight white turbidity. To synthesize bio-TiO2/Algae complex, 0.0053 g L-Arginine powder, and 0.01 g L-Cysteine were dissolved in 20 mL T. obliquus suspensions and stirred for 15 min. After adding 200 μL Ti-BALDH (0.294 wt%) and stirring for another 20 min, slight white turbidity was formed.
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2

OCMC Nanoparticles for Drug Delivery

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OCMC (NO. C914893) was obtained from Shanghai Macklin Biochemical Co., Ltd. β-CD, lapatinib, L-histidine and L-arginine were purchased from Shanghai Macklin Biochemical Co., Ltd. N, N-dimethylformamide (DMF, NO. 227056) was bought from Merck Co., Ltd. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, NO. M8180) was bought from Beijing Solarbio Science Technology Co., ltd. FTIR spectra were analyzed using Shimadzu IRPrestige-21 equipment. TEM images were recorded on a Hitachi-7700 transmission electron microscope. Ultrapure water was obtained using a UPT-II-5T water purification system (Ulupure, Chengdu, China). The diameter and zeta potential of the copolymer were measured on a Malvern Zetasizer Nano ZS-90 technique. Cells were contained in DMEM media with 10% FBS and 1% penicillin/streptomycin, and OD value was analyzed on a Microplate Reader (DYNEX, Series1SPA-0093). Ginsenoside Rg1 (NO. PHR2200) was obtained from Merck Co., Ltd. with purity over 98%.
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3

Amino Acid and Compound Acquisition Protocol

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L-Alanine (CAS No. 56-41-7), L-Arginine (CAS No. 74-79-3), L-Asparagine (CAS No. 70-47-3), L-Aspartic acid (CAS No. 56-84-8), L-Cysteine (CAS No. 52-90-4), L-Glutamic acid (CAS No. 56-86-0), L-Glutamine (CAS No. 56-85-9), L-Histidine (CAS No. 71-00-1), L-Isoleucine (CAS No. 73-32-5), L-Leucine (CAS No. 61-90-5), L-Lysine (CAS No. 56-87-1), L-Methionine (CAS No. 63-68-3), L-Phenylalanine (CAS No. 63-91-2), L-Proline (CAS No. 147-87-3), L-Serine (CAS No. 56-45-1), L-Threonine (CAS No. 72-19-5), L-Tryptophan (CAS No. 73-22-3), L-Tyrosine (CAS No. 60-18-4), L-Valine (CAS No. 72-18-4), and Glycine (CAS No. 56-40-6) were purchased from MACKLIN. MHY1485 (CAS No. 326914-06-1) and Rapamycin (CAS No. 53123-88-9) were purchased from APExBIO.
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4

Electrochemical Characterization of CB-GO Composites

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The CB (Carbon Vulcan XC-72) and GO solutions (mean radial dimension: 5–8 μm, thickness: 1 nm, concentration: 2 mg/mL, dispersed in H2O), Tyr (99%, 60-18-4, 25 g), sarcosine (99%, 25 g, 107-97-1), L-serine (99%, 100 g, 56-45-1), L-arginine (98%, 100 g, 74-79-3), L-threonine (99%, 100 g, 72-19-5), DL-alanine (99%, 100 g, 302-72-7), and N-dimethylformamide (DMF; AR, 99.5%, 500 mL) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Ethyl alcohol and phosphate buffer (PBS) were purchased from Sigma Aldrich (St Louis, MO, USA). Ag/AgCl, CP, and Pt electrodes were purchased from Lanlike Chemical Electronic High Technology Co., Ltd (Tianjin, China). Nafion D520 dispersion liquid was purchased from Dupont Co., Ltd (Wilmington, DE, USA). Electrochemical measurements were performed at ambient temperature (20–25 °C). Deionized (DI) water (~18.0 MΩ) was obtained from a Millipore system.
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