At the maturity stage, rice plants of ten representative hill in each treatment were collected and dried out at 80°C for the measurement of dry matter weight and determination of total nitrogen, phosphorus and potassium accumulation in aboveground tissue according to the methods described by Pan et al.22 . The rice plants collected were divided into leaf, stems and grain and then oven-dried at 80 °C, weighed, ground into powder for digestion. The digested samples were used to determine the total N content by the Kjeldahl method with a 2300 Kjeltec Analyser Unit (Foss Tecator AB, Swedish). The total P and K concentrations were determined by using the UV-VIS Spectrophotometer UV-2550 (SHIMADZU Corporation) and the Atomic Absorption Spectrophotometer AA-6300C (SHIMADZU Corporation) method, respectively.
Uv 2550 uv vis spectrophotometer
Manufactured by Shimadzu
Sourced in Japan, China
The UV-2550 UV-Vis spectrophotometer is a laboratory instrument manufactured by Shimadzu. It is designed to measure the absorption of ultraviolet and visible light by samples. The core function of the UV-2550 is to analyze the spectral characteristics of various substances.
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Lab products found in correlation
Nicolet 6700, by Thermo Fisher Scientific (3 mentions)
D8 advance x ray diffractometer, by Bruker (3 mentions)
Escalab 250, by Thermo Fisher Scientific (3 mentions)
Lsm 700, by Zeiss (2 mentions)
Ls 55 fluorescence spectrophotometer, by PerkinElmer (2 mentions)
Escalab 250 x ray photoelectron spectrometer, by Thermo Fisher Scientific (2 mentions)
Jem 2100f, by JEOL (2 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (2 mentions)
Microplate reader, by Agilent Technologies (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
S 4800, by Hitachi (2 mentions)
Nanodrop 2000, by Thermo Fisher Scientific (2 mentions)
Escalab 250 spectrometer, by Thermo Fisher Scientific (2 mentions)
Atomic absorption spectrophotometer aa6300c, by Shimadzu (1 mentions)
Lumina fluorescence spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Agilent 1100 series, by Agilent Technologies (1 mentions)
Pe 2400, by PerkinElmer (1 mentions)
Raman spectrometer, by Renishaw (1 mentions)
Ultrafiltration tube, by Merck Group (1 mentions)
Desalting column, by GE Healthcare (1 mentions)
72 protocols using uv 2550 uv vis spectrophotometer
1
Rice Plant Nutrient Accumulation Analysis
2
Glucose Measurement and Cell Growth Assay
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Samples were collected every 24 h for measurement of residual glucose. One milliliter culture was centrifuged at 13,000 rpm. Supernatant was used for determination of residual glucose in the medium. Glucose was analyzed using Dionex ICS-3000 Ion Chromatography equipped with CarboPac TM PA20 analytical column and CarboPac TM PA20 guard column (Xiong et al., 2016 (link)). Glucose concentration was quantified by using the external standard method.
The cell growth was determined by measuring the absorbance at 600 nm of the culture using Shimadzu UV-Vis spectrophotometer, UV-2550. For cell dry weight (CDW) measurement, 5 ml of cell culture was collected and centrifuged at 13,000 rpm for 10 min. The pellets were washed twice with 5 ml of distilled water and dried at 104°C, until a consistent weight was obtained (approximately 24 h). The data of weight of dried cell biomass were recorded.
The cell growth was determined by measuring the absorbance at 600 nm of the culture using Shimadzu UV-Vis spectrophotometer, UV-2550. For cell dry weight (CDW) measurement, 5 ml of cell culture was collected and centrifuged at 13,000 rpm for 10 min. The pellets were washed twice with 5 ml of distilled water and dried at 104°C, until a consistent weight was obtained (approximately 24 h). The data of weight of dried cell biomass were recorded.
3
Spectroscopic Characterization of Compounds
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Absorption spectra measurements were performed on a Shimadzu UV-vis spectrophotometer, UV-2550. Fluorescent spectra measurements were performed on a Thermo Scientific Lumina fluorescence spectrophotometer. The pH was carried out by a PB 10 digital pH meter. 1H NMR (400 MHz) and 13C NMR (125 MHz) spectra were measured on a Bruker ACF-300 spectrometer (Bruker Corp., Billerica, MA, USA) using TMS as internal reference. Mass spectra was carried on an Agilent 1100 Series (Agilent Technologies, Santa Clara, CA, USA) LC/MSD high performance ion trap mass spectrometer and a Mariner ESI-TOF spectrometer. All starting materials and reagents were purchased from commercial sources and used without further purification. Deionized water was prepared through a water purification system. Fluorescent microscopy imaging was carried out by a confocal laser scanning microscope (CLSM, LSM700, Zeiss, Germany). Fluorescent images of mice were taken by an IVIS Spectrum.
4
Quantifying Lignin Reduction and Organic Carbon
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Acid soluble lignin and CHN analysis was done on nondegraded samples as well as samples obtained from batch scale tests and 6-gallon laboratory reactors at the end of reactor operations. The differences in these values were used to calculate the percent reductions in lignin shown in the results section. To analyze the solids for acid soluble lignin, a known weight (0.3 g) of ground sample was subjected to two-stage acid hydrolysis as follows. In the first stage, the sample was hydrolyzed in 3 mL of 72% sulfuric acid for 1 h at 30 °C. This was followed by a secondary hydrolysis after the addition of 83 mL of deionized water and a Fucose internal standard. The secondary hydrolysis was conducted in an autoclave (121 °C) for 1 h.
Acid soluble lignin was determined from UV absorbance at 215 nm of the filtered (0.45 mm) acid hydrolysate from the second stage digestion. For this purpose, a Shimadzu UV-VIS spectrophotometer (UV-2550, serial No. A108446) was used. Acid Soluble lignin was measured at 280 nm and 215 nm, and the lignin content was calculated by the following formula:
2.5.2. Total organic carbon Total Organic Carbon was measured for samples at the beginning and end of batch-scale and 6-gallon reactor operation with a CHN analyzer (Perkin-Elmer PE 2400). All samples were acid washed (1 M HCL) to eliminate inorganic carbon prior to analysis (Ryba et al., 2002) .
Acid soluble lignin was determined from UV absorbance at 215 nm of the filtered (0.45 mm) acid hydrolysate from the second stage digestion. For this purpose, a Shimadzu UV-VIS spectrophotometer (UV-2550, serial No. A108446) was used. Acid Soluble lignin was measured at 280 nm and 215 nm, and the lignin content was calculated by the following formula:
2.5.2. Total organic carbon Total Organic Carbon was measured for samples at the beginning and end of batch-scale and 6-gallon reactor operation with a CHN analyzer (Perkin-Elmer PE 2400). All samples were acid washed (1 M HCL) to eliminate inorganic carbon prior to analysis (Ryba et al., 2002) .
5
Comprehensive Characterization of Composite Materials
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SEM (JSM-IT300, Japan) operating at an acceleration voltage 10 kV was used to analyze morphology and the structure of the composites. Before SEM analysis, the samples were ground into powder and dried under vacuum for 24 h. Raman scattering was performed on a laser Raman spectrometer (RENISHAW, Wotton-under-Edge, UK) at 633-nm laser excitation of a He–Ne laser. Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR, Nicolet 6700, Thermo Fisher, Waltham, MA, USA) was used to characterize the structure of the composites. The surface areas of the composites were obtained using the Langmuir single-layer adsorption technique. Absorption spectra were analyzed in a UV-Vis spectrophotometer (UV-2550, Shimadzu, Japan). The particle size was determined by nano series dynamic light scattering (DLS Malvern instruments Co, UK). The dynamic light scattering was measured at an angle of 173°. A He–Ne power laser was used, operating at a wavelength of 633 nm and a voltage of 22 mV.
6
Spectroscopic and Chromatographic Analysis
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UV–VIS spectrophotometer (UV-2550; Shimadzu, Kyoto, Japan); LS-55 fluorescence spectrophotometer (Perkin Elmer, Waltham, MA, USA); ultrafiltration tube (Millipore, Billerica, MA, USA); desalting column (GE Healthcare, Piscataway, NJ, USA); immune chromatography test paper (Wuhan Jiayuan Quantum Dots Co., Ltd., Wuhan, China); gel imaging system (Alpha Imager HP).
7
Turbidimetric Glycopolymer-Lectin Binding
8
Analytical Techniques for Compound Characterization
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A semi micro balance (0.01 mg; Mettler Instrument Co., LTD., Shanghai, China), a PHS-25 pH meter (INESA Scientific Instrument Co., Ltd, Shanghai, China), a UV-2550 UV-vis spectrophotometer (Shimadzu, Kyoto, Japan), an LC-2010 HPLC system (Shimadzu) equipped with a Phenomenex Luna C18 analytical column (250 mm × 4.6 mm, 5 µm), a 6520 Accurate-Mass Q-TOF LC/MS system (Agilent, Santa Clara, CA, USA) and Omega Nanosep ultrafilter centrifuge with a 10-kDa molecular weight cut-off ultrafiltration membrane (Pall Corp., Port Washington, NY, USA) were obtained for this study.
9
Nanoparticle Characterization Techniques
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Ammonium tetrathiomolybdate (≥99.0%), glucose, acetic acid (≥99.5%), sodium acetate (≥99.0%), ferric sulfate hydrate, and hydrogen peroxide (30%, V/V) were all obtained from Kelong Chemical Co., Ltd. (Chengdu, China). CPT and 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB) were ordered from Aladdin Chemical Co., Ltd. (Shanghai, China).
A Shimadzu UV-2550 UV–vis spectrophotometer (Kyoto, Japan) was used to record absorption spectra. A Brookhaven Nano Brook Omni (New York City, NY, USA) was used to measure the size of nanoparticles in solution phase. An FEI Tecnai G2 F20 transmission electron microscope (TEM, Hillsboro, OR, USA) was employed to acquire the TEM images. A Bruker Dimension Icon atomic force microscope (AFM, Billerica, MA, USA) was used to record AFM images. A Thermo Fisher Scientific ESCALAB 250 X-ray photoelectron spectrometer (Waltham, MA, USA) was employed to obtain X-ray photoelectron spectroscopy (XPS) data. A PerkinElmer LS-55 fluorescence spectrophotometer (Waltham, MA, USA) was used to measure the fluorescence spectra. An iPhone 8 smartphone (Cupertino, CA, USA) was used for capturing the photographs of the sample solutions, and an open-source app called Color Grab (Shenzhen, China) was installed in the phone for data readout from the photographs.
A Shimadzu UV-2550 UV–vis spectrophotometer (Kyoto, Japan) was used to record absorption spectra. A Brookhaven Nano Brook Omni (New York City, NY, USA) was used to measure the size of nanoparticles in solution phase. An FEI Tecnai G2 F20 transmission electron microscope (TEM, Hillsboro, OR, USA) was employed to acquire the TEM images. A Bruker Dimension Icon atomic force microscope (AFM, Billerica, MA, USA) was used to record AFM images. A Thermo Fisher Scientific ESCALAB 250 X-ray photoelectron spectrometer (Waltham, MA, USA) was employed to obtain X-ray photoelectron spectroscopy (XPS) data. A PerkinElmer LS-55 fluorescence spectrophotometer (Waltham, MA, USA) was used to measure the fluorescence spectra. An iPhone 8 smartphone (Cupertino, CA, USA) was used for capturing the photographs of the sample solutions, and an open-source app called Color Grab (Shenzhen, China) was installed in the phone for data readout from the photographs.
10
Monitoring AzoDiTAB Cleavage by GSH
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Measurements for GSH-mediated Cleavage of AzoDiTAB were performed on a Shimadzu UV-2550 UV-Vis spectrophotometer using trace quartz cuvettes (d = 10 mm). The spectra signals were recorded in the 240–500 nm wavelength range at a middle speed. 50 μM AzoDiTAB was incubated with 10 mM or 0.5 mM GSH, and the incubation samples were detected in a time range from 0 h (control) to 107 h. Before the measurement, the signal of the buffer was subtracted from the spectra of the samples.
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