Advia centaur autoanalyzer

Blood samples at baseline and 8 years after BS were collected after 12 h fasting. Serum was aliquoted and immediately stored at −80 °C. Serum glucose, cholesterol, triglycerides and HDL were analysed using an Advia Chemistry XPT autoanalyzer (Siemens Healthcare Diagnostics, Malvern, PA, USA). The LDL was calculated using the Friedewald equation. Serum insulin levels were measured via immunoassay using an ADVIA Centaur autoanalyzer (Siemens Healthcare Diagnostics, Malvern, PA, USA). Serum proteins, such as adhesion molecules (L-selectin, PECAM, ICAM-1, VCAM-1), metalloproteinases (MMP3, MMP9), growth factors (VEGF-A VEGF-D), adipokines (adiponectin, leptin), macrophage-related proteins (CD14, CD44, MBL, LVYE1) and other proteins (lactoferrin, elafin, NGAL, NRP1, PAI-1, RANTES) were analysed using ProcartaPlex immunoassay kit (Thermo Fisher Scientific Inc., Rockford, IL, USA) at baseline and 8 years after BS.

Study participants were advised to fast with an effect from 10 p.m. Participants completed a structured interview to obtain demographic and clinical data, including height, waist circumference (WC) and blood pressure. Fasting blood samples were collected before 10:00 a.m. and were allowed to clot at room temperature for 30 min prior to centrifugation (3000 rpm for 10 min). Serum was immediately removed and distributed in aliquots. All samples were collected in the Outpatient Clinic of the Endocrinology Department of the Virgen de la Victoria Hospital, and were immediately transported to the Medical Laboratory Department where they were bar-coded and blinded to patient identification. We used the homeostatic model assessment of insulin resistance (HOMA-IR) to determine the status of insulin resistance [22 (link)]. In non-diabetic men, 1.85 was considered as the cut-off value of insulin resistance [23 (link)]. Serum insulin levels were measured by immunoassay using an ADVIA Centaur autoanalyzer (Siemens, Erlangen, Germany). Weight and body composition were obtained using the Tanita multi-frequency body composition analyzer MC-180MA (Tanita Corporation, Tokyo, Japan).

Blood samples were obtained from the antecubital vein after an overnight fast and placed in vacutainer tubes after an overnight fast. Excessive agitation of blood specimens was avoided to minimize hemolysis and serum was immediately separated from the blood samples by centrifugation for 10 min at 4000 rpm and frozen at −80 °C until analysis. Enzymatic methods (Randox Laboratories Ltd., Crumlin, UK) were employed to analyze the levels of serum cholesterol, triglycerides, HDL-cholesterol, glucose, and glycosylated hemoglobin (HbA1c) using a Dimension Vista autoanalyzer (Siemens Healthcare Diagnostics, Erlangen, Germany). Serum insulin levels were measured by immunoassay using an ADVIA Centaur autoanalyzer (Siemens Healthcare Diagnostics). Insulin resistance (IR) was calculated from the homeostasis model assessment of IR (HOMA-IR) with the formula: HOMA-IR = [fasting serum insulin (μU/mL) × fasting blood glucose (mmol/L)]/22.5.