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2 protocols using anti bfgf

1

Comprehensive Protein Expression Analysis

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The primary antibodies used were anti-collagen I, anti-VEGF and anti-PDGFBB (Abcam, Cambridge, UK); anti-TSG101, anti-CD9, anti-CD63, and anti-CD81 (System Biosciences, Mountain View, CA, USA). The primary antibodies anti-CD41, anti-TGF-β, anti-bFGF, anti-cleaved-caspase-3, anti-Runx2, anti-β-catenin, anti-β-tubulin, anti-calnexin, anti-Bcl-2, anti-CHOP, anti-Bad and phosphorylated Bad (p-Bad), anti-Erk1/2 and phosphorylated Erk1/2 (p-Erk1/2), anti-Akt and phosphorylated Akt (p-Akt) antibody were all obtained from Cell Signaling Technology (Danvers, MA, USA). The anti-PERK and phosphorylated PERK (p-PERK) antibodies were obtained from Santa Cruz Biotechnology.
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2

Extracellular Vesicle Protein Analysis

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The primary antibodies used were anti-VEGF and anti-PDGFBB (Abcam, Cambridge, UK); anti-CD9, anti-CD63, anti-CD81 (System Biosciences); anti-CD41, anti-TGF-β, anti-bFGF, anti-YAP and phosphorylated YAP (p-YAP), anti-Erk1/2 and phosphorylated Erk1/2 (p- Erk1/2), anti-Akt and phosphorylated Akt (p-Akt) (Cell Signaling Technology).
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