Mbs723281

Manufactured by MyBioSource

The MBS723281 is a laboratory equipment designed for [core function]. It is intended for use in [application domain]. The product specifications and technical details are available upon request.

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Lab products found in correlation

3 protocols using mbs723281

Biomarker Quantification in Plasma

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Commercially available ELISA kits were used to measure the concentrations or activity of IFNβ (DIFNB0 or 42400–1, R&D Systems), F3 (DCF300, R&D Systems; ab214091, Abcam), fibrin (MBS265263 or MBS706338, MyBioSource), D-dimer (ab196269, Abcam; MBS723281, MyBioSource), TNF (DTA00D, R&D Systems), IL1A (DLA50, R&D Systems), IL1B (DLB50, R&D Systems), IL6 (D6050, R&D Systems), and HMGB1 (ST51011, IBL International) in the indicated samples. Measurement of GPT/ALT and BUN in the plasma was performed using an IDEXX Catalyst Dx Chemistry Analyzer. PT, APTT, and fibrinogen were measured in an automated coagulometer (Sysmex CA-7000). Platelet count was measured using an IDEXX ProCyte Dx Hematology Analyzer.

Zhang H., Zeng L., Xie M., Liu J., Zhou B., Wu R., Cao L., Kroemer G., Wang H., Billiar T.R., Zeh H.J., Kang R., Jiang J., Yu Y, & Tang D. (2020). TMEM173 Drives Lethal Coagulation in Sepsis. Cell host & microbe, 27(4), 556-570.e6.

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Measuring Biomarkers in Biological Samples

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Commercially available ELISA kits were used to measure the concentrations or activity of HMGB1 (ST51011, Shino Test Corporation), IL1B (MLB00C, R&D Systems), IL6 (M6000B, R&D Systems), TNF (MTA00B, R&D Systems), D-dimer (ab196269, Abcam; MBS723281, MyBioSource), SQSTM1 (ADI-900–212, Enzo Life Sciences), and lactate (ab65331, Abcam; E4341, BioVision) in the indicated samples. Measurement of tissue enzymes (CK, AMY, and GPT/ALT) and BUN in the serum was performed using the IDEXX Catalyst Dx Chemistry Analyzer. Measurement of glucose uptake was performed using a Glucose Uptake Assay Kit (ab136955, Abcam). PT, APTT, and fibrinogen were measured in an automated coagulometer (Sysmex CA-7000). Platelet count was measured using an IDEXX ProCyte Dx Hematology Analyzer.

Zhou B., Liu J., Zeng L., Zhu S., Wang H., Billiar T.R., Kroemer G., Klionsky D.J., Zeh H.J., Jiang J., Tang D, & Kang R. (2020). Extracellular SQSTM1 mediates bacterial septic death in mice through insulin receptor signaling. Nature microbiology, 5(12), 1576-1587.

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Quantification of Metabolic Markers

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Commercially available ELISA kits were used to measure the concentrations of D-dimer (MBS723281, MyBioSource), HMGB1 (ST51011, IBL International), and SQSTM1 (ADI-900-212, Enzo) in the indicated samples. Measurement of ALT and BUN in the plasma was performed using a Catalyst Dx Chemistry Analyzer (IDEXX). Intracellular itaconate concentration was assayed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method (Tan et al., 2020 (link)). In short, to minimize the chance of cell metabolite degradation, cells were lysed by adding ice-cold 80% methanol/water (v/v). The internal standard solution (¹³C₅-itaconate; sc-495554, Santa Cruz) was also prepared in 80% methanol/water. Samples and standards were analyzed using a Triple Quad 5500 LC-MS/MS system (SCIEX).

Chen F., Wu R., Liu J., Kang R., Li J, & Tang D. (2022). The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity. iScience, 25(7), 104561.

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