Paraffin-embedded 4-µm tumor tissue sections were used for immunohistochemical staining. The sections were dewaxed, placed in 3% H2O2 for 15 min at room temperature and washed 3 times with PBS. Then, the sections were incubated in 0.1 mol/l citrate buffer (pH 6.0) for 10 min at 100°C. The samples were probed with a primary antibody against Ki67 (1:100; SAB5300423; Sigma-Aldrich; Merck KGaA), cleaved caspase-3 (1:500; MAB4703, Sigma-Aldrich; Merck KGaA), VEGF (1:100; SAB1402390; Sigma-Aldrich; Merck KGaA) and MMP-9 (1:100; SAB5200294; Sigma-Aldrich; Merck KGaA) overnight at 4°C and then incubated with HRP-conjugated IgG (I-10677; 1:500; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature for 1 h. Positive cells were observed using a light microscope (magnification, ×200) and evaluation was performed using Photoshop CS6 (Adobe Systems, Inc.) and Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).
Sab5200294
Manufactured by Merck Group
Sourced in United States
The SAB5200294 is a laboratory equipment product manufactured by Merck Group. It is a specialized device designed for use in research and scientific applications. The core function of this equipment is to perform specific tasks or analyses, but a detailed description cannot be provided while maintaining an unbiased and factual approach without further interpretation or extrapolation.
Automatically generated - may contain errors
2 protocols using sab5200294
1
Immunohistochemical Evaluation of Tumor Markers
2
Histological Assessment of Carotid Arteries
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The 2–3 mm long common carotid arterial tissues were obtained 1 mm near the carotid bifurcation and were fixed for pathologic evaluation using a 4% PFA solution. Cross-sections of 5 µm were obtained 1 mm proximal to the bifurcation and were stained with H&E and VVG for elastin. Duplicate frozen sections were used for immunohistochemical staining. Briefly, sections were incubated with primary antibodies against oxidized low-density lipoprotein (ox-LDL, 1:500, TA336722, OriGene Technologies, Rockville, MD, USA), CD68 (1:10, NB100-683, Novus-Biologicals, Littleton, CO, USA), and MMP-9 (1:500, SAB5200294, Sigma-Aldrich). The sections were incubated with a biotinylated secondary antibody. An avidin-biotin complex kit (Cell and Tissue Staining Kit, R&D System, Minneapolis, MN 55413, USA) was used for detection.
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