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Discovery ultra xt

Manufactured by Roche
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The Discovery Ultra XT is a high-performance laboratory instrument designed for advanced analytical applications. It features a modular design and powerful capabilities for precise measurement and data analysis. The core function of the Discovery Ultra XT is to provide accurate and reliable results for a wide range of laboratory testing and research needs.

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Lab products found in correlation

Asp300s tissue processor, by Leica (2 mentions) Eosin y, by Thermo Fisher Scientific (2 mentions) Asp300, by Leica (1 mentions) Z fix solution, by Anatech (1 mentions) Picrosirius red, by Polysciences (1 mentions) Dako autostainer plus, by Agilent Technologies (1 mentions) Prolong diamond antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Bx53 f microscope, by Leica (1 mentions) Ab15580, by Abcam (1 mentions) Mayer s hematoxylin, by Merck Group (1 mentions)

Close spelling variants of this product

Discovery XT (190 citations) Discovery Ultra (119 citations) Discovery Ultra XT autostainer (3 citations)

4 protocols using discovery ultra xt

1

Tissue Harvesting and Processing for IHC

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Mice were killed by CO2 asphyxiation, and tissue was quickly collected and fixed overnight at room temperature with Z-fix solution (Anatech). Tissues were processed using a Leica ASP300S tissue processor, paraffin embedded and cut into 5-µm sections. Immunohistochemistry was performed on a Discovery Ultra XT autostainer (Ventana Medical Systems) and counterstained with hematoxylin. Hematoxylin and eosin staining were performed per the manufacturer’s instructions. Human PDA tissue was obtained from deidentified individuals through the University of Michigan Central Biorepository.
Halbrook C.J., Thurston G., Boyer S., Anaraki C., Jiménez J.A., McCarthy A., Steele N.G., Kerk S.A., Hong H.S., Lin L., Law F.V., Felton C., Scipioni L., Sajjakulnukit P., Andren A., Beutel A.K., Singh R., Nelson B.S., Van Den Bergh F., Krall A.S., Mullen P.J., Zhang L., Batra S., Morton J.P., Stanger B.Z., Christofk H.R., Digman M.A., Beard D.A., Viale A., Zhang J., Crawford H.C., Pasca di Magliano M., Jorgensen C, & Lyssiotis C.A. (2022). Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells. Nature Cancer, 3(11), 1386-1403.
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2

Immunohistochemical Analysis of Pancreatic Tissue

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Pancreata were fixed overnight in 4% PFA. Tissues were processed in a Leica ASP300S Tissue Processor, paraffin-embedded and cut into 5 μm sections. IHC for phospho-Akt (T308), p110α, Vav1, Tiam1 and CK19 was performed on a Discovery Ultra XT autostainer (Ventana Medical Systems) and counterstained with hematoxylin. Dual IHC for CK19 and Amylase was performed without counterstain. IHC staining for RAC1 was performed on a DAKO Autostainer Plus (DAKO North America, Inc.) and counterstained with hematoxylin. Picrosirius red staining was performed per manufacturer’s instructions (Polysciences, Inc,). Hemotoxylin and Eosin staining was performed using Mayer’s Hemotoxylin solution (Sigma) and Eosin Y (Fisher).
Wu C.Y., Carpenter E.S., Takeuchi K.K., Halbrook C.J., Peverley L.V., Bien H., Hall J.C., DelGiorno K.E., Pal D., Song Y., Shi C., Lin R.Z, & Crawford H.C. (2014). PI3K Regulation of RAC1 is Required for KRAS-induced Pancreatic Tumorigenesis in Mice. Gastroenterology, 147(6), 1405-1416.e7.
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3

Comprehensive Histological and Immunofluorescent Analyses

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Tissues were fixed overnight in 10% neutral-buffered formalin, dehydrated, and paraffin-embedded. Hematoxylin and eosin and Gomori Trichrome (Thermo Fisher, #87021) staining was performed according to the manufacturer's guidelines. IHC was performed using a Ventana Discovery Ultra XT autostainer. For immunofluorescence staining, deparaffinized slides were blocked with 1% bovine serum albumin in PBS for 1 hour at RT. Primary antibody was diluted in blocking buffer and incubated overnight at 4°C, followed by secondary antibody (Alexa Fluor secondaries, 1:300) for 45 minutes at RT. Nuclei were counterstained with Prolong Diamond Antifade Mountant with DAPI (Invitrogen). Antibodies are found in Supplementary Table S1. Inverted fluorescence images were taken on an Olympus BX53F microscope and confocal images on either Leica SP5 or SP8 microscopes. ImageJ, Fiji V2.0.0-rc-69/1.52p was used for quantitation using at least three 20× magnification fields across three or more biological replicates.
Kemp S.B., Carpenter E.S., Steele N.G., Donahue K.L., Nwosu Z.C., Pacheco A., Velez-Delgado A., Menjivar R.E., Lima F., The S., Espinoza C.E., Brown K., Long D., Lyssiotis C.A., Rao A., Zhang Y., Pasca di Magliano M, & Crawford H.C. (2021). Apolipoprotein E Promotes Immune Suppression in Pancreatic Cancer through NF-κB–Mediated Production of CXCL1. Cancer Research, 81(16), 4305-4318.
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4

Immunohistochemical Tissue Analysis

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Tissues were processed using a Leica ASP300S tissue processor (Leica Microsystems). Paraffin-embedded tissues were sectioned at 4 μm and stained for specific target proteins using the Discovery Ultra XT autostainer (Ventana Medical Systems), with listed antibodies, and counterstained with Mayer’s hematoxylin (Sigma). H&E staining was performed using Mayer’s hematoxylin solution and Eosin Y (Thermo Fisher). IHC slides were then scanned on a Pannoramic SCAN scanner (Perkin Elmer). Scanned images were quantified using algorithms provided from Halo software version 2.0 (Indica Labs). The following antibodies were used for IHC: Ki67 (1:1000; Abcam, ab15580), αSMA (1:20,000; Abcam, ab5694), and Got2 (1:500; Atlas, HPA018139).
Kerk S.A., Lin L., Myers A.L., Sutton D.J., Andren A., Sajjakulnukit P., Zhang L., Zhang Y., Jiménez J.A., Nelson B.S., Chen B., Robinson A., Thurston G., Kemp S.B., Steele N.G., Hoffman M.T., Wen H.J., Long D., Ackenhusen S.E., Ramos J., Gao X., Nwosu Z.C., Galban S., Halbrook C.J., Lombard D.B., Piwnica-Worms D.R., Ying H., Pasca di Magliano M., Crawford H.C., Shah Y.M, & Lyssiotis C.A. (2022). Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context. eLife, 11, e73245.
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