Cells were incubated with trypsin for 3 min in order to harvest them. The pellets were washed, spun at 1200 rpm for 5 min, and re-suspended in 1% BSA in 1X PBS incubation buffer. Cells were spun and treated with mouse Fc block (BD Biosciences, NJ, USA, cat#: 553142) for 20 min. Either FITC anti-mouse MHCI antibody (BD Biosciences, NJ, USA, cat#: 553565) or its corresponding FITC Mouse Isotype control (BD Biosciences, NJ, USA, cat#: 553456) was added to cells and allowed to incubate for 1 h. The samples were fixed with 2% paraformaldehyde for 30 min at 4 °C, washed, and re-suspended in 1X PBS. Analysis was done using the BD FACs instrumentation and software version 7.0 (BD Biosciences, NJ, USA).
Fitc mouse isotype control
Manufactured by BD
Sourced in United States
The FITC Mouse Isotype control is a laboratory reagent used to establish the appropriate level of fluorescence in flow cytometry experiments. It serves as a control to determine the background fluorescence levels for a specific fluorochrome-conjugated antibody.
Automatically generated - may contain errors
2 protocols using fitc mouse isotype control
1
Flow Cytometric Analysis of MHCI Expression
2
Measurement of Fetal Hemoglobin Expression in Erythroid Progenitors
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!