Geimsa

Manufactured by Merck Group

Sourced in United States

Geimsa is a laboratory stain used for the differential staining of blood cells. It is a complex mixture of dyes that selectively stains various components of cells, allowing for the identification and differentiation of different types of blood cells under a microscope.

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Lab products found in correlation

Carboxymethylcellulose, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Methocult gfm3434 culture medium, by STEMCELL (1 mentions) Ribonuclease a rnase a, by Merck Group (1 mentions)

Close spelling variants of this product

Geimsa solution (4 citations) Geimsa stain (2 citations) Wright Geimsa stain (2 citations)

6 protocols using geimsa

Limiting Dilution Assay for siRNA Transfection

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At indicated incubation times following siRNA transfection, cells were replated
at limiting dilution, fed every other day and stained with Geimsa (Sigma).

Maxfield K.E., Taus P.J., Corcoran K., Wooten J., Macion J., Zhou Y., Borromeo M., Kollipara R.K., Yan J., Xie Y., Xie X.J, & Whitehurst A.W. (2015). Comprehensive functional characterization of cancer–testis antigens defines obligate participation in multiple hallmarks of cancer. Nature Communications, 6, 8840.

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Migration Assay with Fibronectin

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The amount of 750 µl culture media containing 20µl/ml fibronectin without serum was added to the bottom wells of matrigel 24 well plates (SPL Life Sciences, Korea). Well inserts was applied on wells gently to prevent bubbling in the bottom. The treated cells were tripsynised and after a brief spin, 2x10⁴ cells were counted and seed to each well insert of 24 well plates with 500 µl of media containing 10% FBS and incubated at 37˚C in 5% CO₂ incubator for 24 hours. Inserts were removed and fixed with 3.7% paraformaldehyde for 20 minutes after rinsing with PBS. Fixed cells were stained with Geimsa (Sigma, USA) and noninvasive cells were removed with a cotton swab. The remaining migrated cells were observed by using light microscopy (Ziess, Germany) counting 10 different fields with 10x of magnification.

Abolhassani A., Riazi G.H., Azizi E., Amanpour S., Muhammadnejad S., Haddadi M., Zekri A, & Shirkoohi R. (2014). FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines. Journal of Cancer, 5(7), 537-547.

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Plaque Assay for Virus Quantification

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1×10⁵ HFF cells were plated into wells of 12-well plates and cultured overnight. 1×10⁴ THP-1 cells were harvested at six days post-infection and co-cultured with HFFs for 2 days. Culture media was then removed and the plates were washed twice with 1× PBS. Cell monolayers were covered with overlay media (a 1:1 mixture of 1.5% carboxymethyl cellulose (Sigma), and 2× MEM supplemented with 20% FCIII serum, nonessential amino acids, and penicillin-streptomycin) and incubated for another 8 days to allow for plaque development. Cells were fixed with methanol, stained with 10% Geimsa (Sigma) and plaques were counted using a dissecting microscope.

Wu S.E, & Miller W.E. (2015). The Human Cytomegalovirus Lytic Cycle Is Induced by 1,25-Dihydroxyvitamin D3 in Peripheral Blood Monocytes and in the THP-1 Monocytic Cell Line. Virology, 483, 83-95.

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Plaque Assay for Viral Quantification

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After infection, culture media from each cell sample was harvested on the indicated days, and 10 μl of culture media was added to HFF monolayers plated in 12-well plates the previous day. Virus was adsorbed to HFF monolayers for 3 hours, culture media were removed, and covered with overlay media (a 1:1 mixture of 1.5% carboxymethyl cellulose (Sigma), and 2× MEM supplemented with 20% FCIII serum, nonessential amino acids, and penicillin-streptomycin) and incubated for another 8 days to allow for plaque development. Cells were fixed with methanol, stained with 10% Geimsa (Sigma) and plaques were counted using a dissecting microscope.

Wu S.E, & Miller W.E. (2015). The Human Cytomegalovirus Lytic Cycle Is Induced by 1,25-Dihydroxyvitamin D3 in Peripheral Blood Monocytes and in the THP-1 Monocytic Cell Line. Virology, 483, 83-95.

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Quantifying Leishmania Parasite Burden

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Spleen and liver were isolated from three month infected animals and parasite burden was determined by Leishman Donovan Unit (LDU) and limiting dilution assay (LDA). For LDU, stamp smears of organs were fixed with methanol and stained with Geimsa (Sigma Aldrich, US). LDU was represented by the number of amastigotes calculated in nearly 1000 macrophages from each slide of organ impressions multiplied by the respective organ weight in milligram (mg).
For LDA calculation, a 1 mg/ml (w/v) organ suspension (spleen or liver) prepared in Schneider drosophila media was serially diluted and cultured at 22°C for 2 weeks. Parasite burden was expressed as 10-fold logarithm scale of the highest dilution containing viable parasites, and the mean values of three mice per group were represented.

Das S., Mukherjee S, & Ali N. (2021). Super enhancer-mediated transcription of miR146a-5p drives M2 polarization during Leishmania donovani infection. PLoS Pathogens, 17(2), e1009343.

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DAMTC Synthesis and Characterization

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7, 8-diacetoxy-4-methylthiocoumarin (DAMTC), whose synthesis has been reported earlier52 (link) was obtained from the Department of Chemistry, University of Delhi, Delhi, India. Dimethyl sulfoxide (DMSO), Geimsa, Propidium iodide (PI), Ribonuclease-A (RNase-A) were obtained from Sigma-Aldrich Chemical Co., St. Louis, MO, USA. Methocult GF M3434 culture medium was purchased from Stem cell technologies, Canada. Antibodies to mouse F4/80 (FITC), MHC-II (PE), CD11b (APC) and CD86 (PerCP Cy5.5) were procured from eBiosciences, USA. Phosphate buffer saline (PBS), Ethylene diaminetetracetic acid (EDTA) and all other chemicals obtained were of analytical grade from BDH, Glaxo laboratories (Qualigens), SRL and E-Merck, India.

Venkateswaran K., Shrivastava A., Agrawala P.K., Prasad A., Kalra N., Pandey P.R., Manda K., Raj H.G., Parmar V.S, & Dwarakanath B.S. (2016). Mitigation of radiation-induced hematopoietic injury by the polyphenolic acetate 7, 8-diacetoxy-4-methylthiocoumarin in mice. Scientific Reports, 6, 37305.

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