The following antibodies were used: HDAC6 (H-300; Santa Cruz), acetyl-α-tubulin (Sigma), ubiquitin (Biomol), LAMP-1 (Hybridoma Bank, Iowa), GFP (Roche), active-caspase 3 (Millipore), human SOD1 (Millipore), and actin (Sigma).
Acetyl α tubulin
Manufactured by Merck Group
Sourced in United States
Acetyl-α-tubulin is a laboratory reagent used in research applications. It is a structural component of microtubules, which are cytoskeletal filaments involved in various cellular processes. Acetyl-α-tubulin is a post-translationally modified form of the alpha-tubulin protein, with an acetyl group added to a specific lysine residue. This modification can influence microtubule dynamics and stability. Acetyl-α-tubulin is commonly used as a marker for studying microtubule-related cellular functions.
Automatically generated - may contain errors
Lab products found in correlation
α tubulin, by Merck Group (2 mentions)
Active caspase 3, by Merck Group (1 mentions)
Hdac6 h 300, by Santa Cruz Biotechnology (1 mentions)
Mouse monoclonal anti β catenin, by BD (1 mentions)
Mouse monoclonal anti α tubulin, by Merck Group (1 mentions)
Mouse monoclonal anti rhoa, by Abcam (1 mentions)
Anti gst, by OriGene (1 mentions)
C jun, by Abcam (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti sox9, by Merck Group (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Cyclin antibody sampler kit, by Cell Signaling Technology (1 mentions)
Twist1, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Ac smc3, by Merck Group (1 mentions)
Hdac8, by Abcam (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gapdh, by Bioworld Technology (1 mentions)
6 protocols using acetyl α tubulin
1
Antibody-based Protein Detection
2
Characterization of LRP6 Signaling Pathway
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The mouse monoclonal anti-LRP6 antibody was from Santa Cruz (Santa Cruz, CA, USA). Rabbit polyclonal anti-p-LRP6 (Ser1490) was from Bioss (Woburn, MA, USA). Mouse monoclonal anti-β-catenin was from BD (Franklin Lakes, NJ, USA). Anti-phalloidin was from Life (Carlsbad, CA, USA). Mouse monoclonal anti-vinculin was from Boster (Wuhan, China). Mouse monoclonal anti-α-tubulin, acetyl-α-tubulin, tyrosine-α-tubulin were from Sigma-Aldrich (Saint Louis, MO, USA). Rabbit polyclonal anti-detyrosinated-α-tubulin, Mouse monoclonal anti-RhoA, Rac1and Cdc42 were purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal anti-phosphor-MAP1B (Thr1265) was from Novusbio (Littleton, CO, USA). Rabbit polyclonal anti-MACF1 was from Proteintech (Chicago, IL, USA). Rabbit monoclonal anti-phosphor-GSK3β (Ser9) and mouse monoclonal anti-GSK3β were from Cell Signaling (Beverly, MA, USA). Rabbit polyclonal anti-His, mouse monoclonal anti-His and anti-GST were from Origene (Rockville, MD, USA).
3
Comprehensive Protein Analysis by Western Blot
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Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer composed of 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.1% SDS, 10 mM NaF, 1 mM Na3VO4, and protease inhibitor cocktail (Roche, Switzerland). The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Merck, Germany) and detected using antibodies against acetyl-α-tubulin (T6199; Sigma), p-JNK (4668; Cell Signaling Technology [CST], USA), JNK (AHO1362; Invitrogen, USA), p-ERK (9106; CST), ERK (4696; CST), p-p38 (9215; CST), p38 (9212; CST), p-c-Jun (3270; CST), c-Jun (ab32137; Abcam, UK), c-Fos (2250; CST), p-ATF2 (27934; CST), ATF2 (35031; CST), Bcl2 (4223; CST), p-Histone H3 (9701; CST), p-cdc2 (4539; CST), p-Elk1 (9181; CST), α-tubulin (T6199; Sigma), and cyclin antibody sampler kit (9869; CST).
4
Cellular Characterization and Immunoblotting
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HT29 and RKO cells were cultured in McCoy’s 5A medium supplemented with 10% FBS, 1% glutamine, and 1% penicillin/streptomycin. HEK 293 Lentix cells were cultured in DMEM medium supplemented with 10% FBS, 1% glutamine, and 1% penicillin/streptomycin.
Anti–β-catenin, -Elp1, and -Hsp90 antibodies used in Western blot analyses were obtained from Santa Cruz Biotechnology, Inc. The anti-Elp3 antibody was provided by Cell Signaling Technology. Anti–α-tubulin and –acetyl-α-tubulin antibodies were purchased from Sigma-Aldrich. Anti-Histone H3, -pH2AX, and -Dclk1 antibodies were obtained from Abcam, and anti-Sox9 and Ephrin B2 antibodies were purchased from EMD Millipore and R&D Systems, respectively.
Anti–β-catenin, -Elp1, and -Hsp90 antibodies used in Western blot analyses were obtained from Santa Cruz Biotechnology, Inc. The anti-Elp3 antibody was provided by Cell Signaling Technology. Anti–α-tubulin and –acetyl-α-tubulin antibodies were purchased from Sigma-Aldrich. Anti-Histone H3, -pH2AX, and -Dclk1 antibodies were obtained from Abcam, and anti-Sox9 and Ephrin B2 antibodies were purchased from EMD Millipore and R&D Systems, respectively.
5
Protein Extraction and Immunoblotting
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Collected cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 1% SDS, 0.25% sodium deoxycholate, 10 mM NaF, 1 mM Na3VO4, and protease inhibitor cocktail (Roche, Switzerland). Primary antibodies against acetyl-α-tubulin (Sigma), α-tubulin (Sigma), Cyclin Antibody Sampler Kit (Cell Signaling Technology, Danvers, MA, USA), and Acetyl-Histone H3 Sampler Kit (Cell Signaling Technology, USA) were used.
6
Evaluating Anticancer Agents and Targets
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PCI-34051 and ACY-241 (Citarinostat) were obtained from CSNpharm (Arlington Heights, IL, USA). All agents were dissolved in DMSO (Sigma Chemical, St. Louis, MO, USA). The following antibodies were used: GAPDH (AP0066, Bioworld Technology, Bloomington, MN, USA), tubulin (sc-32293, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bak (sc-832, Santa Cruz Biotechnology), SMC3 (sc-376352, Santa Cruz Biotechnology), p53 (sc-126, Santa Cruz Biotechnology), p21 (sc-53870, Santa Cruz Biotechnology), PARP (551024, BD Biosciences, San Jose, CA, USA), XIAP (610716, BD Biosciences), MMP-9 (A0289, ABclonal Technology, Woburn, MA, USA), caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA), Bcl-xL (#2762, Cell Signaling Technology), TWIST1 (#46702, Cell Signaling Technology), acetyl α-tubulin (T6793, Sigma-Aldrich, St. Louis, MO, USA), ZEB1 (HPA027524, Sigma-Aldrich), HDAC6 (A301-342A-M, Bethyl Laboratories, Montgomery, TX, USA), HDAC8 (ab187139, Abcam, Cambridge, UK), and ac-SMC3 (MABE1073, Millipore, Burlington, MA, USA).
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