Blood was recovered in Vacutainer glass collection tubes with heparin (BD Biosciences). PBMC were isolated by gradient centrifugation with Histopaque-1077. Plasma was recovered for cytokine quantification by cytokine bead array (BD Biosciences) and resistin ELISA (Peprotech). Cell aliquots were frozen in liquid nitrogen. Following blood draw, PBMC isolation was performed within 24 hours through density gradient centrifugation, and cells were stored immediately in liquid nitrogen. Flow cytometry characterization of PBMC involved incubation with Human TruStain FcX™(Biolegend) and staining with primary antibodies: CD14(HCD14, Biolegend), CD16(3G8, Biolegend), CD66b(G10F5, eBioscience), CD3(OKT3, eBioScience). Samples were acquired on a BD LSRII and analyzed on FlowJo (v10).
Cd66b g10f5
Manufactured by Thermo Fisher Scientific
CD66b(G10F5) is a monoclonal antibody that recognizes the CD66b antigen. CD66b is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of mature human granulocytes, including neutrophils, eosinophils, and basophils. The CD66b(G10F5) antibody can be used for the identification and enumeration of these cell types in flow cytometric applications.
Automatically generated - may contain errors
Lab products found in correlation
Human trustain fcx, by BioLegend (2 mentions)
Vacutainer glass collection tubes with heparin, by BD (2 mentions)
Resistin elisa, by Thermo Fisher Scientific (2 mentions)
Cd14 hcd14, by BioLegend (2 mentions)
Cd16 3g8, by BioLegend (2 mentions)
Lsr 2, by BD (2 mentions)
Cytokine bead array, by BD (2 mentions)
2 protocols using cd66b g10f5
1
PBMC Isolation and Characterization
2
Comprehensive PBMC Isolation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood was recovered in Vacutainer glass collection tubes with heparin (BD Biosciences). PBMC were isolated by gradient centrifugation with Histopaque-1077. Plasma was recovered for cytokine quantification by cytokine bead array (BD Biosciences) and resistin ELISA (Peprotech). Cell aliquots were frozen in liquid nitrogen. Following blood draw, PBMC isolation was performed within 24 h through density gradient centrifugation, and cells were stored immediately in liquid nitrogen. Flow cytometry characterization of PBMC involved incubation with Human TruStain FcX™ (Biolegend) and staining with primary Abs: CD14 (HCD14, Biolegend), CD16 (3G8, Biolegend), CD66b (G10F5, eBioscience), CD3 (OKT3, eBioScience). Samples were acquired on a BD LSRII and analyzed on FlowJo (v10).
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