Serum and CSF NfL were measured with the Simoa Human Nf-Light Advantage kit using a fully automated SIMOA® HD-1 analyzer based on a Single-Molecule Array technology (Quanterix, Lexington, MA, United States) (Sampedro et al., 2020 (link)). Quantitative measurements of NfL in blood by Simoa technology have been shown to be reliable (Soylu-Kucharz et al., 2017 (link)). More details on the processing of samples in the cohort can be read in the biologic’s manual for PPMI1 (Kang et al., 2013 (link)).
Human nf light advantage kit
Manufactured by Quanterix
Sourced in United States
The Human NF-light Advantage kit is a laboratory equipment product designed for the quantitative detection and measurement of neurofilament light (NF-light) proteins in human samples. The kit utilizes an immunoassay-based approach to provide accurate and reliable data on NF-light levels, which are associated with various neurological conditions.
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Lab products found in correlation
3 protocols using human nf light advantage kit
1
Quantification of Neurofilament Light in Biofluid
2
Quantifying Serum NfL Levels in PPMI Cohort
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Serum NfL levels of the PPMI cohort were assessed using the Simoa Human NF-light Advantage kit using Single Molecule Array technology in a completely automated SIMOA® HD-1 analyzer (Quanterix, Lexington, MA, USA) [17 (link)]. The Simoa technology provides a reliable quantitative assay of blood NfL levels [18 , 19 (link)]. Detailed information on the handling of the samples may be accessed in the manual of the PPMI (http://www.ppmi-info.org ).
3
Serum Neurofilament Light Biomarker Measurement
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Serum NfL concentrations were measured at baseline, 0.5-, 1-, 2-, 3-, and 5-year intervals using the Simoa Human NF-light Advantage kit with the Single Molecule Array in a fully automated SIMOA® HD-1 analyzer (Quanterix, Lexington, MA, USA). The details of the samples are available in the PPMI biologic manual (http://www.ppmi-info.org ).
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