Log In Sign up
The largest database of trusted experimental protocols

Anti k48 ubiquitin

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Anti-K48 ubiquitin is a monoclonal antibody that specifically recognizes the K48-linked polyubiquitin chains. Ubiquitin is a small regulatory protein that can be covalently attached to other proteins, marking them for degradation by the proteasome.

Automatically generated - may contain errors

Lab products found in correlation

Integrin α5, by BD (2 mentions) Anti mmp9, by Novus Biologicals (2 mentions) Anti sirt1, by Cell Signaling Technology (2 mentions) Anti mmp2, by Cell Signaling Technology (2 mentions) Srt1720, by Selleck Chemicals (2 mentions) Anti rl2, by Santa Cruz Biotechnology (2 mentions) P erk1 2, by Merck Group (2 mentions) Anti ogt, by Merck Group (2 mentions) Anti o glcnac, by Merck Group (2 mentions) Anti praptor 792, by Cell Signaling Technology (2 mentions) Ex527, by Selleck Chemicals (2 mentions) Anti foxm1, by Santa Cruz Biotechnology (2 mentions) Anti raptor, by Cell Signaling Technology (2 mentions) Compound c, by Selleck Chemicals (2 mentions) Mg132, by Merck Group (2 mentions) Anti actin, by Santa Cruz Biotechnology (2 mentions) Anti p27, by Cell Signaling Technology (2 mentions) Anti k63 ubiquitin, by Merck Group (2 mentions) Ab129068, by Abcam (1 mentions) Immobilon pvdf membrane, by Merck Group (1 mentions)

Close spelling variants of this product

Ubiquitin (177 citations) Anti-ubiquitin (116 citations) K48-Ubiquitin (8 citations) Anti-K48 (7 citations) Anti-K48-linked ubiquitin (3 citations)

8 protocols using anti k48 ubiquitin

1

Protein Modification and Regulation Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
NButGT, Ac-5SGlcNAc were provided by D. Vocadlo (Simon Fraser University)(48 (link)). SRT1720, EX-527, Compound C purchased from Selleck-Chemicals (Houston, TX). MG132 purchased from Sigma-Aldrich (St. Louis, MO). pcDNA3.1-SIRT1-Flag was gift from E. Verdin (Addgene plasmid#: 13812). pLenti4-HA-OGT (provided by K. Vosseller, Drexel University). Antibodies used: anti-actin, anti-FOXM1 and anti-RL2 from Santa Cruz Biotechnology; pERK1/2, anti-OGT and anti-O-GlcNAc from Sigma-Aldrich; anti-acetylated p53(K382), anti-AMPK, anti-pAMPK(S172), anti-ERK1/2, anti-MMP2, anti-pRaptor(792), anti-Raptor, anti-SIRT1 and anti-Ubiquitin(K48) from Cell Signaling (Danvers, MA); anti-Cdh1 and anti-p53 from Neobiolabs; anti-MMP9 from Novus-Biologicals (Littleton, CO); integrin α5 and α6 are from BD-Pharmingen (San Jose, CA).
Ferrer C.M., Lu T.Y., Bacigalupa Z.A., Katsetos C.D., Sinclair D.A, & Reginato M.J. (2016). O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway. Oncogene, 36(4), 559-569.
+ Open protocol
+ Expand
2

Protein Modification and Regulation Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
NButGT, Ac-5SGlcNAc were provided by D. Vocadlo (Simon Fraser University)(48 (link)). SRT1720, EX-527, Compound C purchased from Selleck-Chemicals (Houston, TX). MG132 purchased from Sigma-Aldrich (St. Louis, MO). pcDNA3.1-SIRT1-Flag was gift from E. Verdin (Addgene plasmid#: 13812). pLenti4-HA-OGT (provided by K. Vosseller, Drexel University). Antibodies used: anti-actin, anti-FOXM1 and anti-RL2 from Santa Cruz Biotechnology; pERK1/2, anti-OGT and anti-O-GlcNAc from Sigma-Aldrich; anti-acetylated p53(K382), anti-AMPK, anti-pAMPK(S172), anti-ERK1/2, anti-MMP2, anti-pRaptor(792), anti-Raptor, anti-SIRT1 and anti-Ubiquitin(K48) from Cell Signaling (Danvers, MA); anti-Cdh1 and anti-p53 from Neobiolabs; anti-MMP9 from Novus-Biologicals (Littleton, CO); integrin α5 and α6 are from BD-Pharmingen (San Jose, CA).
Ferrer C.M., Lu T.Y., Bacigalupa Z.A., Katsetos C.D., Sinclair D.A, & Reginato M.J. (2016). O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway. Oncogene, 36(4), 559-569.
+ Open protocol
+ Expand
3

Western Blot Analysis of Zymosan-Stimulated RAW264.7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RAW264.7 cells were stimulated with zymosan (50 μg ml−1) for the indicated time points, washed in PBS and lysed (1% NP-40, 100 mM Tris, 100 mM NaCl, pH 8.0). Lysates were resolved by PAGE, transferred to Immobilon PVDF membranes (Millipore) and subjected to western blot. Primary antibodies were mouse anti-gp91phox (611415, BD Pharmingen, dilution 1:1,000), mouse anti-pERK (9106S, Cell Signaling Technology, dilution 1:1,000) and rabbit anti-ERK2 (sc-154, Santa Cruz Biotechnology, dilution 1:2,000). Secondary antibodies were horseradish peroxidase conjugates of anti-mouse IgG and anti-rabbit IgG (7074S and 7076S, Cell Signaling Technology dilution, 1:5,000). For immunoprecipitation experiments, lysates were incubated overnight with protein A/G-coupled magnetic beads (Pierce) coupled to rabbit anti-gp91phox (ab129068, Abcam, 5 μg per sample). Western blots were performed as described above with anti-Ubiquitin K48 (12805S, Cell Signaling Technology, dilution 1:1,000).
Graham D.B., Becker C.E., Doan A., Goel G., Villablanca E.J., Knights D., Mok A., Ng A.C., Doench J.G., Root D.E., Clish C.B, & Xavier R.J. (2015). Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst. Nature Communications, 6, 7838.
+ Open protocol
+ Expand
4

Ubiquitin-mediated protein regulation assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spautin-1 (S7888), Imatinib (S1026), and MG132 (S2619) were from Sellectchem (Houston, TX, USA). SKP2-C25 (M60136) was from Xcessbio Biosciences, Inc. (San Diego, CA). MTS reagent (G3582) was from Promega Corporation (Madison, WI, USA). Co-IP assay kit (14311D) was from Life Technologies (Carlsbad, CA). Antibodies: anti-Ubiquitin (#3936), anti-K63-Ubiquitin (#12930), anti-K48-Ubiquitin (#12805), anti-USP10 (#8501), anti-USP1 (#8033), anti-USP2 (#8036), anti-USP7 (#4833), anti-USP8 (#11832), anti-USP14 (#11931), anti-USP15 (#66310), anti-USP18 (#4813), anti-UCHL1 (#13179), anti-UCHL3 (#8141), anti-CYLD (#8462), anti-A20 (#5630), anti-SKP2 (#2652), anti-p27 (#3686), anti-FLAG (#14793), anti-HA (#3724), anti-phospho-c-Abl(Y245) (#2861), anti-c-Abl (#2862), anti-phospho-STAT5 (#9359), anti-STAT5 (#25656), anti-phospho-Crkl (#3181) and anti-Crkl (#38710) (Cell Signaling Technology, Beverly, MA, USA); anti-UCHL5 (ab124931), anti-USP13 (ab109264) (Abcam, Cambridge, MA); anti-GAPDH (BS60630) (Bioworld Technology, Inc., Louis Park, MN, USA).
Liao Y., Liu N., Xia X., Guo Z., Li Y., Jiang L., Zhou R., Tang D., Huang H, & Liu J. (2019). USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia. Cell Discovery, 5, 24.
+ Open protocol
+ Expand
5

CIITA Ubiquitination Study in COS Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
COS cells were plated at cell density of 8 × 105/10 cm on tissue culture plates. Cells were transfected using GeneJuice (Merck Millipore, Darmstadt, Germany) as indicated with 5 μg of Myc-CIITA, Flag-pCAF, Flag-K141R, K144R, K141/144R CIITA, HA-K48 Ub, K63 Ub, HA-mono Ub, or pCDNA control. Twenty-four hours after transfections, cells were lysed in 1% NP40 buffer supplemented with EDTA-free protease inhibitors (Roche) on ice. Lysates were centrifuged, normalized for protein concentration, and precleared with Mouse IgG (Sigma-Aldrich) and Protein G (Thermo Fisher) followed by immunoprecipitation with either EZ view anti-c Myc affinity gel beads (Sigma-Aldrich) or with anti-Flag M2 affinity gel (Sigma-Aldrich). Immune complexes were denatured with Laemmli buffer, boiled, and separated by SDS-PAGE gel electrophoresis. Gels were transferred to nitrocellulose and were individually immunoblotted with anti-Myc (Abcam, Cambridge, MA), anti-Flag (Sigma-Aldrich), antiubiquitin (Life Sensors, Malvern, PA), anti-K48 ubiquitin (Cell Signaling, Danvers, MA), anti-K63 ubiquitin (Millipore), or with anti-GST (Abcam, Cambridge, MA). HRP conjugates were detected using HyGlo Chemiluminescent substrate (Denville). Protein normalization and equal loading were determined in lysates.
Morgan J.E, & Greer S.F. (2017). Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator. International Journal of Cell Biology, 2017, 8093813.
+ Open protocol
+ Expand
6

Comprehensive Protein Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated by standard 10% SDS-PAGE, with samples loaded in Laemmli buffer, transferred for 2h to a PVDF membrane, and immunoblotted using the following antibodies: anti-K63 ubiquitin (1:4,000; EMD Millipore, cat. No. 05–1308, clone apu3); anti-GAPDH (1:4,000; Abcam, cat. No. ab9485); anti-Rps10 (1:6,000, Sigma, cat no. WH0004736M1); anti-Rps6 (1:4,000; Abcam cat no. ab40820), anti-puromycin (1,2:500, EMD Millipore MABE343, clone 12D10), anti HA (1:2,500; Invitrogen, cat no. 71–5500), anti-K48 ubiquitin (1:8,000; Cell Signaling, cat. No 8081).
Back S., Gorman A.W., Vogel C, & Silva G.M. (2018). Site-specific K63 ubiquitinomics provides insights into translation regulation under stress. Journal of proteome research, 18(1), 309-318.
+ Open protocol
+ Expand
7

Immunoblotting Assay for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following antibodies were used: anti-FBXO31 (F4431), anti-FLAG (F1840), anti-phosphoserine (P5747), anti-phosphothreonine (P6623) and anti-tubulin (T5168) from Sigma. Anti-FBXL20 (TA306520) and anti-DDK (TA50011) from Origene. Anti-myc (#11667149001) from Roche. Anti-His (sc-8036), anti-cleaved PARP-1(sc-56196), anti-HA (sc-7392), anti-BIM (sc-374358), anti-GFP (sc-9996), anti-p53 (sc-126) anti-GSKa/b (sc-7291) from Santacruz. Anti-PUMA (#4976), BAX (#2772), anti-AKT1 (#9272), anti-pAKT473 (#4058), anti-cleaved Caspase-9 (#9501), anti-K48-ubiquitin (#8081), anti-GST(#2624), anti-BCL2 (#4223) from Cell Signaling technology.
Manne R.K., Agrawal Y., Malonia S.K., Banday S., Edachery S., Patel A., Kumar A., Shetty P, & Santra M.K. (2021). FBXL20 promotes breast cancer malignancy by inhibiting apoptosis through degradation of PUMA and BAX. The Journal of Biological Chemistry, 297(4), 101253.
+ Open protocol
+ Expand
8

Proteomic Analysis of Androgen Receptor Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemicals: natural product library (#L1400), rutaecarpine (#S2349), parecoxib (#S4656), bortezomib (#S1013), MG132 (#S2619), enzalutamide (#S1250), and bicalutamide (#S1190) were obtained from Selleckchem (Houston, TX). Antibodies: anti-AR-V7 (#19672), anti-AR (#5153), anti-K48-ubiquitin (#12805), anti-IgG (#3900), anti-MDM2 (#86934), anti-CDK2 (#2546), anti-CDK4 (#12790), anti-CDK6 (#13331), anti-cyclinD1 (#2978), anti-p21 (#2947), anti-p27 (#3686), anti-p15 (#4822), anti-COX2 (#12282), anti-HA-tag (#2367), anti-FLAG-tag (#8146), anti-GAPDH (#5174), anti-GRP94 (#20292), anti-HSP90 (#4877), and anti-lamin B1(#13435) were obtained from Cell Signaling Technology (Beverly, MA); anti-GRP78 (#ab21685), anti-HSP7C (#ab51052), anti-SIAH2 (#ab230532), anti-STUB1 (#ab134064), anti-AR-V7 (#ab198394, for IHC), and anti-Ki67 (#ab15580) were obtained from Abcam (Cambridge, MA).
Liao Y., Liu Y., Xia X., Shao Z., Huang C., He J., Jiang L., Tang D., Liu J, & Huang H. (2020). Targeting GRP78-dependent AR-V7 protein degradation overcomes castration-resistance in prostate cancer therapy. Theranostics, 10(8), 3366-3381.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!