High sensitivity enzyme linked immunosorbent assay kits

Manufactured by R&D Systems

Sourced in United Kingdom

High-sensitivity enzyme-linked immunosorbent assay (ELISA) kits are laboratory tools designed to detect and quantify specific proteins or molecules in a sample. These kits utilize antibodies and enzyme-based detection methods to provide a sensitive and accurate analysis of target analytes.

Automatically generated - may contain errors

Lab products found in correlation

Orthofusion 5.1 f s analysers, by Ortho Clinical Diagnostics (1 mentions) Tops coagulometer, by Werfen (1 mentions) Huvecs, by R&D Systems (1 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions) Softmax pro 5, by Molecular Devices (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Au5810 chemistry analyzers, by Beckman Coulter (1 mentions)

Spelling variants of this product

High-sensitivity enzyme-linked immunosorbent assay (12 citations) High sensitivity kits (4 citations) Quantikine High Sensitivity Enzyme-linked Immunosorbent Assay (ELISA) kits (3 citations)

5 protocols using high sensitivity enzyme linked immunosorbent assay kits

Aging, Inflammation, and Cardiometabolic Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were drawn from participants at age 70-wave 1 (baseline) and at a 3-year follow-up at age 73-Wave 2, by a qualified nurse, immediately centrifuged, and stored frozen at −80°C. Serum CRP (mg/l) and fibrinogen (g/l) concentrations were measured at ages 70 and 73. High-sensitivity CRP (hs-CRP mg/l) and IL-6 (pg/l) concentrations were measured at age 73 only.
The (non-hs) CRP assay was performed using a dry-slide immuno-rate method on OrthoFusion 5.1 F.S analysers (Ortho Clinical Diagnostics). The CRP assay method has low sensitivity in the lower range of CRP values; approximately 45% of participants were in the single lowest category (1.5 mg/l). The fibrinogen assay was performed using an automated Clauss assay (TOPS coagulometer; Instrumentation Laboratory, Warrington, UK). Hs-CRP and IL-6 were determined using high-sensitivity enzyme-linked immunosorbent assay kits (R&D Systems, Oxon, UK).

Corley J., Shivappa N., Hébert J.R., Starr J.M, & Deary I.J. (2019). Associations between Dietary Inflammatory Index Scores and Inflammatory Biomarkers among Older Adults in the Lothian Birth Cohort 1936 Study. The Journal of Nutrition, Health & Aging, 23(7), 628-636.

+ Open protocol

+ Expand

Quantification of CXCL8, IL-1β, and MPO

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levels of CXC-chemokine ligand 8 (CXCL8), interleukin (IL)-1β, and myeloperoxidase (MPO) in the sputum supernatants were measured using high sensitivity enzyme-linked immunosorbent assay kits (R&D Systems, Abingdon, UK). The lower limit of detection were 3.5 pg.ml^-1, <1.0 pg.ml^-1 and 0.014 ng.ml^-1 for CXCL8, IL-1β, and MPO respectively.

Singh R., Mackay A.J., Patel A.R., Garcha D.S., Kowlessar B.S., Brill S.E., Donnelly L.E., Barnes P.J., Donaldson G.C, & Wedzicha J.A. (2014). Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. Respiratory Research, 15(1), 114.

+ Open protocol

+ Expand

Angiogenic Growth Factors in ECM/SVF Gel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum samples were collected from each mouse at all time points. Expressions of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and monocyte chemotactic protein-1 (MCP-1) in ECM/SVF-gel extracts or serum were determined by high sensitivity enzyme-linked immunosorbent assay kits (R&D Systems). For tube formation assay, human umbilical vein endothelial cells (HUVECs) were obtained from the Research Laboratory Collaboration Alliance of Nanfang Hospital (Guangzhou, China). Culture plate and the experimental equipment were precooled at −20°C before operation; Matrigel (Becton, Dickinson & Co., 356230, Franklin Lakes, NJ) was added to the culture plate and solidified by incubation at 37°C for 2 h before seeding cells. HUVECs (2 × 10⁴ per well) mixed with 1 mL of ECM/SVF-gel extract from 7-day samples were then seeded in the culture plate. As a positive control, the HUVECs in the culture plate were treated with angiogenic growth factors (10 ng/mL vascular endothelial growth factor (VEGF; R&D Systems, MN, USA) and 1 ng/mL basic fibroblast growth factor (bFGF; Sigma)). As a control, HUVECs alone were cultured in normal culture medium. Angiogenesis (tube formation) was observed and photographed in high-powered fields (HPF) after 24 h.

Sun M., He Y., Zhou T., Zhang P., Gao J, & Lu F. (2017). Adipose Extracellular Matrix/Stromal Vascular Fraction Gel Secretes Angiogenic Factors and Enhances Skin Wound Healing in a Murine Model. BioMed Research International, 2017, 3105780.

+ Open protocol

+ Expand

Plasma IL-6 Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood was drawn into Vacutainer tubes containing EDTA as anticoagulant and was immediately centrifuged at 1246×g at room temperature for 10 min. The resulting supernatant was aliquoted in cryo vials and stored at −80 °C until assay. Samples were analyzed in duplicate using high-sensitivity enzyme-linked immunosorbent assay kits (R&D System, Oxford, UK) characterized by a minimum detectable dose range of 0.016–0.110 pg/mL and intra/inter-assay coefficients of variation of 7 and 7.2 %, respectively. IL-6 concentrations were determined with a microplate reader (Molecular Devices, UK) and SoftMax Pro 5 software using a four-parameter logistic curve-fit reduction of the raw absorbance data in accordance with the protocol.

Endrighi R., Hamer M, & Steptoe A. (2016). Post-menopausal Women Exhibit Greater Interleukin-6 Responses to Mental Stress Than Older Men. Annals of Behavioral Medicine, 50, 564-571.

+ Open protocol

+ Expand

Comprehensive Biomarker Analysis of Blood Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected and stored at – 80 °C. The concentration of 25(OH)D was analyzed using Roche Cobas E601 ECL analyzer (Roche, Geneva, Switzerland) and Roche Cobas Vitamin D total assay reagent (Roche Diagnostics GmbH, Mannheim, Germany). Calibration curves were constructed using calibrators provided in the kits.
Hemoglobin and red blood cell count (RBC) were measured through the UniCel DxH 600 Coulter Cellular Analysis System hematology analyzer (Beckman Coulter, Miami, FL). Total iron binding capacity (TIBC) was measured by AU5810 Chemistry Analyzers (Beckman Coulter).
Serum concentrations of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) were detected with high-sensitivity enzyme-linked immunosorbent assay kits (R&D Systems, Minneapolis, USA). Malondialdehyde (MDA) concentration was determined through thiobarbituric acid method and superoxide dismutase (SOD) concentration was determined by pyrogallol autoxidation method, and the kits were purchased from Sichuan Vichy Biotechnology Co. Serum glutathione peroxidase (GSH-Px) concentration was determined by fluorometric assay, and the kit was purchased from Shanghai Yuanye Biotechnology Co. All the internal controls were provided by the kits.

Zhang C., Wang J., Xie X, & Sun D. (2021). Low serum vitamin D concentration is correlated with anemia, microinflammation, and oxidative stress in patients with peritoneal dialysis. Journal of Translational Medicine, 19, 411.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!