Anti mouse cat no ab131368 hrp

SPEs were procured from Metrohm (DRP-250BT). 2-(N-morpholino) ethanesulfonic acid MES), N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide (EDC), Poly (ethylene glycol) 2-mercaptoethanol ether acetic acid (PEG 3500 Da), N-Hydroxysuccinimide (NHS), bovine serum albumin, Halt Protease, and phosphatase inhibitor were procured from Sigma Aldrich(St. Louis, MO, USA). Amersham ECL reagent was obtained from GE Health Care(Chicago, IL, USA). Clot activator tubes and powdered Non-fat milk were procured from local vendors. All other consumables and reagents were reagent grade.
Primary Antibodies: Anti-CD63 (Cat No. PAB25155) was obtained from Abnova for Western blotting. Anti-CD63 (Cat No. BS-1523R) (for functionalizing the SPEs), Anti-HSP70 (Cat No. Mab 33-3800), and Anti-TSG101 (Cat No. PA5-31260) were procured from Thermo Fisher(Waltham, MA, USA). Anti-HER2 (Cat No. SAB4500785) and HER2 Antigen (Cat No. H3040) were procured from Sigma. Anti-CD63 (Cat No. AB134045) and Anti-HER2 (Cat No. Ab16901) (for Western blotting) were procured from Abcam.
Secondary antibodies: Anti-rabbit (Cat No. ab131366) and anti-mouse (Cat No. ab131368) HRP were obtained from Abcam(Cambridge, UK).

Gold (III) chloride hydrate (HAuCl4—Cat No. 254169), trisodium citrate dihydrate (Cat No. S1804), poly(ethylene glycol) 2-mercaptoethanol ether acetic acid (PEG 3500 Da—Cat No. 757837), 2-(N-morpholino) ethanesulfonic acid (MES—Cat No. M5287), N-hydroxysuccinimide (NHS—Cat No. 56480), N-(3-dimethylaminopropyl)-N-ethyl carbodiimide (EDC—Cat No. 39391), and bovine serum albumin (Cat No. 05470) were procured from Sigma-Aldrich (St. Louis, MO, USA). Nonfat milk powder and clot activator tubes (4 mL) were procured from local vendors. Amersham ECL reagent (Cat No. RPN2235) was obtained from GE HealthCare (Chicago, IL, USA). All other reagents and consumables were of reagent grade. Primary antibodies anti-HSP70 (Cat No. MAb 33-3800), anti-CD63 (Cat No. BS-1523R) and anti-calnexin (Cat No. MA5-15389) were procured from Thermo Fisher (Waltham, MA, USA). Secondary antibodies anti-mouse (Cat No. ab131368) HRP and anti-rabbit (Cat No. ab131366) HRP were obtained from Abcam (Cambridge, UK).