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96 well opaque culture plate

Manufactured by BD
Sourced in United States

The 96-well opaque culture plate is a laboratory equipment designed for cell culture applications. It features a 96-well format and an opaque material construction, which helps to minimize light exposure and potential interference with experimental results.

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2 protocols using 96 well opaque culture plate

1

Measuring 3D Cell Viability of Panc-1 Spheroids

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Cell viability of Panc-1 spheroids was measured using a 3D cell viability assay (Promega, Milan, Italy). Briefly, homogeneous spheroids were removed from the 96-well low-attachment culture plate and placed separately in single wells of a 96-well opaque culture plate (BD Falcon). CellTiter-Glo® 3D reagent was added to each well and the luminescence signal was read after 30 min with the GloMax® bioluminescent reader (Promega).
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2

Cell Viability Assay for Silenced Cells

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Cell viability of silenced and naïve cells was measured using a luminescent cell viability assay (Promega, Milan, Italy). Briefly, cells were plated in a 96-well opaque culture plate (BD Falcon, Corning, Somerville, MA, USA), CellTiter-Glo® reagent was added to each well and the luminescence signal was read after 30 min with the GloMax® 96 microplate luminometer (Promega, Milan, Italy).
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