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Anti ddit4

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Anti-DDIT4 is a primary antibody used for the detection and analysis of DDIT4 (DNA Damage Inducible Transcript 4) protein expression in various biological samples. DDIT4 is a stress-responsive protein that plays a role in regulating cellular processes. This antibody can be used in techniques such as Western Blotting to identify and quantify the DDIT4 protein.

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Lab products found in correlation

Anti phospho p70 s6 kinase thr389, by Cell Signaling Technology (2 mentions) Anti p70 s6 kinase, by Cell Signaling Technology (2 mentions) Anti 4e bp1, by Cell Signaling Technology (2 mentions) Anti phospho 4ebp1, by Cell Signaling Technology (2 mentions) Anti human p53 do 1, by Santa Cruz Biotechnology (1 mentions) β actin, by Merck Group (1 mentions) Anti phospho tsc2, by Cell Signaling Technology (1 mentions) Anti phospho gsk3β, by Cell Signaling Technology (1 mentions) Hrp conjugated anti β actin, by Merck Group (1 mentions) Fitc conjugated anti human cd45, by BioLegend (1 mentions) Hrp linked mouse igg, by GE Healthcare (1 mentions) Anti sesn2, by Proteintech (1 mentions) Rabbit igg, by GE Healthcare (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti phospho irs 1, by Cell Signaling Technology (1 mentions) Anti phospho s6 s240 244, by Cell Signaling Technology (1 mentions) Anti nf kb p65, by Cell Signaling Technology (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Apc conjugated anti mouse cd45, by BioLegend (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions)

3 protocols using anti ddit4

1

Investigating Protein Regulation via Western Blot

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Western blot analysis was done using the whole-cell extracts prepared in RIPA buffer. The blot was probed with antibodies of a polyclonal antibody raised against human p53 K139 acetylated peptide, anti-human p53 (DO-1, Santa Cruz), anti-Phospho-p70 S6 kinase (Thr389; Cell Signaling Technologies), anti-p70 S6 kinase (Cell Signaling Technologies 49D7), anti-phospho-4E-BP1 (Ser65; Cell Signaling Technologies 9451), anti-4E-BP1 (Cell Signaling Technologies 9644), anti-DDIT4 (Proteintech 10638-1-AP), and anti-mouse p53 (Novocastra CM5). The levels of β-actin (Sigma-Aldrich A3853) were used as the protein loading control.
Kon N., Ou Y., Wang S.J., Li H., Rustgi A.K, & Gu W. (2021). mTOR inhibition acts as an unexpected checkpoint in p53-mediated tumor suppression. Genes & Development, 35(1-2), 59-64.
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2

Molecular Mechanisms Underlying mTOR Signaling Regulation

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The following antibodies were purchased from Cell Signaling Technology: anti-phospho- IRS1 (S1101 Cat # 2385), anti-phospho-S6 (S240/244, Cat # 5364), anti-phospho-TSC2 (S939 Cat # 3615), anti-TSC2 (Cat # 3990), anti-PTEN (Cat # 138G6), anti-Phospho-NF-kB p65 (S536 Cat # 93H1), anti-NF-kB p65 (Cat # D14E12), anti-phospho-4EBP1 (S65 Cat # 13443), anti-4EBP1(Cat # 9644), and anti-phospho-GSK3β (S9, Cat # 5558). Anti-IRS1 (Cat # 06–248) antibody was purchased from Merck Millipore. Anti-DDIT4 (Cat#10638–1-AP) and anti-SESN2 (Cat # 10795–1-AP) antibodies were purchased from ProteinTech Group. HRP conjugated anti-β-actin (Cat # A3854) was purchased from Sigma. HRP-linked mouse IgG (Cat # NA931V) and rabbit IgG (Cat # NAV934V) were purchased from GE Healthcare Life Sciences. IgG (Isotype control Cat # 400107), FITC conjugated anti-human CD45 (Cat # 304006), and APC conjugated anti-mouse CD45 (Cat # 103112) were purchased from BioLegend. Anti-S6 (Cat # SC74459) antibody was purchased from Santa Cruz laboratories. LGB-321 (Cat # A14420), AZD1208 (Cat # A13203), and IKK16 (# A12836) were purchased from Adooq bioscience. TNF-α (Cat # PHC3015) was purchased from Fisher scientific.
Supplementary Methods include a description of the Western Blot, RNA-Seq data analysis, GSEA analysis, RPPA, Active module analysis, Lentiviral production, the NF-κB reporter assay, and Statistics.
Lim J.T., Singh N., Leuvano L.A., Calvert V.S., Petricoin E.F., Teachey D.T., Lock R.B., Padi M., Kraft A.S, & Padi S.K. (2020). PIM kinase inhibitors block the growth of primary T-cell acute lymphoblastic leukemia: Resistance pathways identified by network modeling analysis. Molecular cancer therapeutics, 19(9), 1809-1821.
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3

Western Blotting Analysis of Signaling Pathways

Cited 1 time
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Cell lysates were prepared and western blotting was performed as described previously (42 (link)). Proteins were separated using 6%, 10%, or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins were detected using the following primary antibodies: anti-phospho-AMPKα (Cell Signaling Technology, clone 40H9), anti-AMPKα (Cell Signaling Technology), anti-phospho-mTOR (Cell Signaling Technology, clone D9C2), anti-mTOR (Cell Signaling Technology, clone 7C10), anti-β-actin (Cell Signaling Technology, clone 13E5), anti-DDIT4 (Proteintech), anti-phospho-Rictor (Cell Signaling Technology, clone D30A3), anti-Rictor (Cell Signaling Technology, clone 53A2), anti-phospho-FoxO3a (Cell Signaling Technology, clone D18H8), anti-FoxO3a (Cell Signaling Technology, clone D19A7), anti-phospho-AKT (Cell Signaling Technology, clone Ser473), anti-AKT (Cell Signaling Technology, clone Ser473), anti-phospho-p70 S6 Kinase (Thr389) (Cell Signaling Technology, 9205S), anti p70 S6 Kinase (Cell Signaling Technology.9202S), anti-phospho-Raptor(Ser792) (Cell Signaling Technology, 2083S), and Raptor (24C12) (Cell Signaling Technology.2280S) antibodies. After the incubating with the corresponding secondary antibody, the ECL substrate (Thermo Fisher) signals were detected using an Amersham Imager 600 (Cytiva Life Sciences, GE HealthCare).
Kim H.E., Lee J.Y., Yoo D.H., Park H.H., Choi E.J., Nam K.H., Park J, & Choi J.K. (2024). Imidazole propionate ameliorates atopic dermatitis-like skin lesions by inhibiting mitochondrial ROS and mTORC2. Frontiers in Immunology, 15, 1324026.
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