DMEM (high glucose; Cat. No. 12100-046) and trypsin-EDTA were from Gibco (Carlsbad, CA, United States). FBS was from HyClone (Logan, UT, United States). Penicillin-streptomycin solution and penicillin-streptomycin-amphotericin B solution were from Biological Industries (Kibbutz Beit Haemek, Israel). Poly (I:C) was from InvivoGen (San Diego, CA, United States). TNF-α was from Biolegend (San Diego, CA, United States). PMA, PBS, mitomycin C, LPS, and H2O2 were from Sigma-Aldrich (St. Louis, MO, United States). Gefitinib was from Selleckchem (Houston, TX, United States). Recombinant human EGF was from PeproTech (Rocky Hill, NJ, United States). Blimp-1 (#9115), p-EGFR (Y1068, #2234) and Syk (#2712) antibodies were from Cell Signaling (Beverly, MA, United States). EGFR antibody (sc-03) was from Santa Cruz (Santa Cruz, CA, United States). p-Syk antibody (PK1010) was from Millipore (Burlington, Ma, United States).
Syk 2712
Manufactured by Cell Signaling Technology
Sourced in United States
Syk (#2712) is a laboratory tool used for the detection and quantification of the Syk protein. The Syk protein is a tyrosine kinase that plays a crucial role in various cellular signaling pathways. The product provided by Cell Signaling Technology allows for the specific identification and measurement of Syk expression levels in cellular samples.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Mitomycin c, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Penicillin streptomycin solution, by Sartorius (1 mentions)
Poly 1 c, by InvivoGen (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
Tnf α, by BioLegend (1 mentions)
Recombinant human egf, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin amphotericin b solution, by Sartorius (1 mentions)
Egfr antibody sc 03, by Santa Cruz Biotechnology (1 mentions)
Las 4000 luminescent image analyzer, by Fujifilm (1 mentions)
Horseradish peroxidase conjugated immunoglobulin g antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using syk 2712
1
EGFR Signaling Pathway Modulation
2
Western Blot Analysis of Syk Phosphorylation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole‐liver proteins were boiled in Laemmli’s buffer. Samples were resolved in 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel under reducing conditions, using a Tris‐glycine buffer system; resolved proteins were transferred onto a nitrocellulose membrane. SYK proteins were detected by specific primary antibodies (SYK, 2712 [Cell Signaling]; phospho‐SYKY525/526, ab58575 [Abcam]) followed by an appropriate secondary horseradish peroxidase‐conjugated immunoglobulin G antibody from Santa Cruz Biotechnology. β‐actin, detected by an ab49900 antibody (Abcam), was used as a loading control. The specific immunoreactive bands of interest were visualized by chemiluminescence (Bio‐Rad Laboratories) using the Fujifilm LAS‐4000 luminescent image analyzer.
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