Epithelial colorectal adenocarcinoma COLO205 CCL-222 tumor cells (ATCC, Manassas VA) in 2D cultures were seeded and maintained in RPMI 1640 cell culture medium supplemented with 10% fetal bovine serum (ATCC) and incubated at 37°C with humidified atmosphere of 95% air and 5% CO2. Cell viability prior and during the NBQ48 treatments was determined with an automated Countess Cell counter from Invitrogen using the presto blue (PB) exclusion method. Prior to treatment, cells were sub cultured and kept at a density of 5.0×105 cells per 3.5mL of culture media plus additives in 25cm2 flasks to assure stable metabolic state and exponential growth.
Colo205 ccl 222 tumor cells
Manufactured by American Type Culture Collection
COLO205 CCL-222 are colorectal adenocarcinoma tumor cells derived from a male Caucasian patient. The cells are adherent and have an epithelial-like morphology.
Automatically generated - may contain errors
2 protocols using colo205 ccl 222 tumor cells
1
Colorectal Adenocarcinoma COLO205 2D Culture
2
Colorectal Adenocarcinoma COLO205 2D Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Epithelial colorectal adenocarcinoma COLO205 CCL-222 tumor cells (ATCC, Manassas VA) in 2D cultures were seeded and maintained in RPMI 1640 cell culture medium supplemented with 10% fetal bovine serum (ATCC) and incubated at 37°C with humidified atmosphere of 95% air and 5% CO2. Cell viability prior and during the NBQ48 treatments was determined with an automated Countess Cell counter from Invitrogen using the presto blue (PB) exclusion method. Prior to treatment, cells were sub cultured and kept at a density of 5.0×105 cells per 3.5mL of culture media plus additives in 25cm2 flasks to assure stable metabolic state and exponential growth.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!